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Evaluation system development for sake koji activity by fluorescence analysis

Research Project

Project/Area Number 23580356
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Agricultural information engineering
Research InstitutionUtsunomiya University

Principal Investigator

SAITO Takahiro  宇都宮大学, 農学部, 教授 (50221990)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords麹菌 / 蛍光 / 活性
Research Abstract

Appropriate excitation and detection wavelengths were chosen for measuring various parameters describing the activity of Aspergillus oryzae. As a result, we found that the best excitation and detection wavelengths for investigating Aspergillus oryzae were 410 and 630 nm, respectively. The fluorescence may be induced by the activity of Aspergillus oryzae because no fluorescence peaks were detected from sterilized rice koji. In addition, we showed that this system was not influenced by the moisture content or glucose concentration, which both changed during the rice koji cultivation.The fluorescence emission strength was closely correlated with both the mycelial weight in rice koji and enzyme activity. Thus, fluorescence spectroscopy should prove suitable for evaluating the mycelia weight in rice koji, amylase activity, and acid carboxypeptidase activity.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (6 results)

All 2013 2012 2011

All Journal Article (1 results) Presentation (5 results)

  • [Journal Article] 蛍光分光法を利用した製麹工程モニタリング技術の開発2013

    • Author(s)
      佐々木隆浩、齋藤高弘
    • Journal Title

      日本醸造協会誌

      Volume: 108(6) Pages: 389-393

    • NAID

      10031177036

    • Related Report
      2013 Final Research Report
  • [Presentation] 機能性の高い味噌の製造方法の開発2013

    • Author(s)
      千葉陽介
    • Organizer
      生態工学会
    • Place of Presentation
      玉川大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 発光法を用いた清酒の品質評価法の開発2012

    • Author(s)
      千葉陽介、片岡皆人、齋藤高弘、志賀徹、星佳宏、佐々木隆浩、岡本竹己
    • Organizer
      2012生態工学会年次大会
    • Related Report
      2013 Final Research Report
  • [Presentation] 化学発光と蛍光を用いた清酒の熟度評価について2012

    • Author(s)
      千葉陽介、齋藤高弘、志賀徹、星佳宏、佐々木隆浩、岡本竹己
    • Organizer
      農業環境工学関連学会2012年合同大会
    • Related Report
      2013 Final Research Report
  • [Presentation] 化学発光と蛍光を用いた清酒の熟度評価について2012

    • Author(s)
      千葉陽介・齋藤高弘・志賀 徹・星 佳宏・佐々木隆浩・岡本竹己
    • Organizer
      農業環境工学関連学会2012年合同大会
    • Place of Presentation
      宇都宮大学
    • Related Report
      2012 Research-status Report
  • [Presentation] ORAC法を指標とした機能性の高い清酒造り2011

    • Author(s)
      阿部泰幸
    • Organizer
      日本醸造学会
    • Place of Presentation
      北とぴあ
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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