Ovarian follicular cell dysfunction by lipopolysaccharide
Project/Area Number |
23580383
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied animal science
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Research Institution | Obihiro University of Agriculture and Veterinary Medicine |
Principal Investigator |
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Project Period (FY) |
2011-04-28 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 卵巣機能 / ステロイドホルモン / 内毒素 / 顆粒層細胞 / 卵胞膜細胞 / 卵胞 / ステロイド産生 / 黄体化 |
Outline of Final Research Achievements |
In postpartum dairy cows, various inflammatory diseases depress reproductive performance. Lipopolysaccharide (LPS) derived from infections of the uterus or mammary gland with Gram-negative bacteria was shown to affect reproduction of dairy cow. LPS concentration detected in follicular fluid ranged from 0.2 to 2.0 EU/mL. In follicles with a high level of LPS (>0.5 EU/mL, n = 15), the concentration of E2 was lower and that of P4 was higher when compared to those in follicles with a low level of LPS (<0.5 EU/mL, n = 23). These findings suggest a possible mechanism of ovarian dysfunction and subsequent infertility in cows with endometritis.
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Report
(5 results)
Research Products
(25 results)
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[Journal Article] The effect of single nucleotide polymorphisms in the tumor necrosis factor-a gene on reproductive performance and immune function in dairy cattle.2014
Author(s)
Kawasaki, Y., Aoki, Y., Magata, F., Miyamoto, A., Kawashima, C., Hojo, T., Okuda, K., Shirasuna, K., Shimizu, T.
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Journal Title
Journal of Reproduction and Development
Volume: 60
Pages: 280-287
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A naturally occurred αs1-casein-derived peptide in bovine milk inhibits apoptosis of granulosa cells induced by serum-free condition.2014
Author(s)
Shimizu, T., Ganzorig, K., Miyamoto, A., Ishii, T., Urashima, T., Fukuda, K.
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Journal Title
J. Pept. Sci.
Volume: 20
Issue: 3
Pages: 229-234
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Evidence that the dominant follicle of the first wave is more active than that of the second wave in terms of its growth rate, blood flow supply and steroidogenic capacity in cows.2014
Author(s)
Miura, R., Haneda, S., Lee, H.H., Miyamoto, A., Shimizu, T., Miyahara, K., Miyake, Y.I., Matsui, M.
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Journal Title
Anim. Reprod. Sci.
Volume: 145
Issue: 3-4
Pages: 114-122
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Effects of lipopolysaccharide (LPS) and peptidoglycan (PGN) on estradiol production in bovine granulosa cells from small and large follicles.2012
Author(s)
Shimizu, T., Miyauchi, K., Shirasuna, K., Bollwein, H., Murayama, C., Miyamoto, A.
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Journal Title
Toxicology In Vitro
Volume: 26
Issue: 7
Pages: 1134-1142
DOI
Related Report
Peer Reviewed
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[Journal Article] Effects of interleukin-8 on estradiol and progesterone production by bovine granulosa cells from large follicles and progesterone production by luteinizing granulosa cells in culture.2012
Author(s)
Shimizu, T., Kaji, A., Imamura, E., Murayama, C., Magata, F., Shirasuna, K., Wakamiya, K., Okuda, K., Miyamoto, A.
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Journal Title
Cytokine
Volume: 57
Pages: 175-181
Related Report
Peer Reviewed
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[Journal Article] Circadian clock genes Per2 and Clock regulate steroid production, cell proliferation, and luteinizing hormone receptor transcription in ovarian granulosa cells.2011
Author(s)
Shimizu, T., Hirai, Y., Murayama, C., Miyamoto, A., Miyazaki, H., Miyazaki, K.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 412
Pages: 132-135
Related Report
Peer Reviewed
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[Presentation] Effect of endotoxin on ovarian follicle function in domestic animals.2015
Author(s)
Shimizu, T., Horiuchi, M., Miyauchi K., Echizenya, R., Magata, F., Miyamoto, A.
Organizer
International Conference on Biology and Pathology of Reproduction in Domestic Animals
Place of Presentation
Gdansk, Poland.
Year and Date
2015-09-28 – 2015-09-30
Related Report
Invited
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