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Research on the development of highly improved method for bioleaching from low-grade sulfide ores

Research Project

Project/Area Number 23580458
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Boundary agriculture
Research InstitutionOkayama University

Principal Investigator

KAMIMURA Kazuo  岡山大学, その他の研究科, 教授 (80294445)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords鉄酸化細菌 / 硫黄酸化 / チオ硫酸デヒドロゲナーゼ / バクテリアリーチング / 応用微生物学 / 遺伝子発現制御 / チオ硫酸酸化
Research Abstract

An iron-oxidizing bacterium, Acidithiobacillus ferrooxidans, is one of the key organisms used in industrial bioleaching applications. Both iron- and sulfur-oxidizing activities are necessary for bioleaching. However, expressions of genes involved in sulfur oxidation are strongly repressed in the presence of ferrous iron. In this research, a protein (ScpB) thought to be involved in the transcriptional regulation of suifide:quinone reductase gene was examined. The transcription of scpB gene was highly activated in sulfur-grown cells. ScpB could bind to putative promoter regions of genes involved in iron or RISC oxidation. Although thiosulfate metabolism in this bacterium has not been characterized, a new gene involved in thiosulfate metabolism has been identified. The properties of the enzyme (thiosulfate dehydrogenase) was characterized.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (37 results)

All 2014 2013 2012 2011 Other

All Journal Article (6 results) (of which Peer Reviewed: 4 results) Presentation (28 results) Remarks (3 results)

  • [Journal Article] 鉄酸化細菌:そのいい加減な生存戦略2014

    • Author(s)
      上村一雄,金尾忠芳
    • Journal Title

      生物工学会誌

      Volume: 92

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Analysis of the microbial community in moderately acidic drainage from the Yanahara pyrite mine in Japan2014

    • Author(s)
      Wang Y, Yasuda T, Sharmin S, Kanao T, Kamimura K.
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 76

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Crystallization and preliminary X-ray diffraction analysis of tetrathionate hydrolase from Acidithiobacillus ferrooxidans2013

    • Author(s)
      Kanao T, Kosaka M, Yoshida K,Nakayama H, Tamada T, Kuroki R, Yamada H, Takada J, Kamimura K.
    • Journal Title

      Acta Crystallographica Section F

      Volume: 69 Issue: 6 Pages: 692-694

    • DOI

      10.1107/s1744309113013419

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tetrathionate-forming thiosulfate dehydrogenase from the acidophilic, chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans.2013

    • Author(s)
      Kikumoto M.
    • Journal Title

      Appl. Environ. Microbiol.

      Volume: 79 Issue: 1 Pages: 113-120

    • DOI

      10.1128/aem.02251-12

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of an OmpA-like outer membrane protein of the acidophilic iron-oxidizing bacte- rium, Acidithiobacillus ferrooxidans2011

    • Author(s)
      Manshur MA, Kikumoto M, Kanao T, Takada J, Kamimura K.
    • Journal Title

      Extremophiles

      Volume: 15 Issue: 3 Pages: 403-410

    • DOI

      10.1007/s00792-011-0371-6

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of an OmpA-like outer membrane protein of the acidophilic iron-oxidizing bacterium, Acidithiobacillus ferrooxidans2011

    • Author(s)
      モハメッド A. マンシュル、菊本 愛生、金尾 忠芳、高田 潤、上村 一雄
    • Journal Title

      Extremophiles

      Volume: 15 Pages: 403-410

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 微生物の硫黄代謝酵素研究の新展開2013

    • Author(s)
      金尾忠芳,上村一雄
    • Organizer
      第65回日本生物工学会大会
    • Place of Presentation
      広島
    • Year and Date
      2013-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] 鉄酸化細菌Acidithiobacillus ferrooxidansのロダネース様タンパク質の機能解析2013

    • Author(s)
      上村一雄,濵崎良太,スルタナシャーミン,金尾忠芳
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      仙台
    • Year and Date
      2013-03-26
    • Related Report
      2013 Final Research Report
  • [Presentation] 鉄硫黄酸化細菌Acidithiobacillus ferrooxidans由来テトラチオン酸ハイドロラーゼの結晶化2013

    • Author(s)
      中山久之,小坂恵,上村一雄,金尾忠芳
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      仙台
    • Year and Date
      2013-03-26
    • Related Report
      2013 Final Research Report
  • [Presentation] 鉄硫黄酸化細菌Acidithiobacillus ferrooxidansにおけるテトラチオン酸ハイドロラーゼ遺伝子の転写と制御に関する研究2013

    • Author(s)
      金尾忠芳,伊藤恵,村上知佐,上村一雄
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      仙台
    • Year and Date
      2013-03-26
    • Related Report
      2013 Final Research Report
  • [Presentation] 中等度酸性環境の微生物層の解析2013

    • Author(s)
      安田貴志,金尾忠芳,上村一雄
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学川内北キャンパス
    • Related Report
      2012 Research-status Report
  • [Presentation] 鉄酸化細菌Acidithiobacillus ferrooxidansのロダネース様タンパク質の機能解析2013

    • Author(s)
      上村一雄,濵崎良太,スルタナシャーミン,金尾忠芳.
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学川内北キャンパス
    • Related Report
      2012 Research-status Report
  • [Presentation] 鉄硫黄酸化細菌Acidithiobacillus ferrooxidans由来テトラチオン酸ハイドロラーゼの結晶化2013

    • Author(s)
      中山久之,小坂 恵,上村一雄,金尾忠芳.
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学川内北キャンパス
    • Related Report
      2012 Research-status Report
  • [Presentation] 鉄硫黄酸化細菌Acidithiobacillus ferrooxidansにおけるテトラチオン酸ハイドロラーゼ遺伝子の転写と制御に関する研究2013

    • Author(s)
      金尾忠芳,伊藤 恵,村上知佐,上村一雄
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学川内北キャンパス
    • Related Report
      2012 Research-status Report
  • [Presentation] Acidithiobacillus ferrooxidansの新規なチオ硫酸デヒドロゲナーゼの性質2012

    • Author(s)
      上村一雄,野上翔平,菊本愛生,高田潤,金尾忠芳
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2012-10-23
    • Related Report
      2013 Final Research Report
  • [Presentation] Acidithio- bacillus ferrooxidansのチオ硫酸デヒドロゲナーゼの構造と機能2012

    • Author(s)
      菊本愛生,野上翔平,襟立佳那,金尾忠芳,高田潤,上村一雄
    • Organizer
      日本農芸学会2012年度大会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Acidithiobacillus ferrooxidansの新規なチオ硫酸デヒドロゲナーゼの性質2012

    • Author(s)
      上村一雄,野上翔平,菊本愛生,高田潤,金尾忠芳.
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] Tetrathionate hydrolase from the marine acidophilic sulfur-oxidizing bacterium Acidithiobacillus thiooxidans strain SH2012

    • Author(s)
      Kanao T., Hashimoto Y., Toge T., Kikukawa H., Kamimura K.
    • Organizer
      9th International Congress on Extremophiles
    • Place of Presentation
      Spain Sevilla
    • Related Report
      2012 Research-status Report
  • [Presentation] Isolation and properties of acidophilic bacteria and the cryptic plasmid from acidic environments2012

    • Author(s)
      Sato T., Kamimura K., Kanao T
    • Organizer
      9th International Congress on Extremophiles
    • Place of Presentation
      Spain Sevilla
    • Related Report
      2012 Research-status Report
  • [Presentation] Acidithiobacillus fetrrooxidansのチオ硫酸デヒドロゲナーゼの構造と機能2012

    • Author(s)
      菊本 愛生、野上 翔平、襟立 佳那、金尾 忠芳、高田 潤、上村一雄
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学(京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] Expression and characterization of a diheme cytochrome c encoded by gene cycA2 from the acidophilic Acidi- thiobacillus ferrooxidans as a recombinant protein in Escherichia coli2011

    • Author(s)
      田崎翔悟,菊本愛生,金尾忠芳,上村一雄
    • Organizer
      International Union of Micro- biological Societies 2011 Congress
    • Place of Presentation
      札幌
    • Year and Date
      2011-09-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Identification of a gene involved in thiosulfate oxidation in an acidophilic iron-oxidizing bacterium Acidithiobacillus ferrooxidans2011

    • Author(s)
      菊本愛生,金尾忠芳,高田潤,上村一雄
    • Organizer
      International Union of Microbiolo- gical Societies 2011 Congress
    • Place of Presentation
      札幌
    • Year and Date
      2011-09-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of a protein involved in the regulation of sulfur and iron oxidation by Acidithiobacillus ferro- oxidans2011

    • Author(s)
      上村一雄,菊本愛生,高田潤,金尾忠芳
    • Organizer
      19^<th> International Biohydro- metallurgy Symposium
    • Place of Presentation
      China
    • Year and Date
      2011-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of a protein involved in the regulation of sulfur and iron oxidation by Acidithiobacillus ferrooxidans2011

    • Author(s)
      上村 一雄、菊本 愛生、高田 潤、金尾 忠芳
    • Organizer
      19th International Biohydrometallurgy Symposium
    • Place of Presentation
      長沙 (中国)
    • Related Report
      2011 Research-status Report
  • [Presentation] Tetrathionate hydrolase is a key enzyme of the dissimilatory sulfur metabolisim in the iron- and sulfur-oxidizing bacterium Acidithiobacillus ferrooxidans2011

    • Author(s)
      金尾 忠芳、上村 一雄
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌コンベンションセンター(札幌)
    • Related Report
      2011 Research-status Report
  • [Presentation] Identification of a gene involved in thiosulfate oxidation in an acidophilic iron-oxidizing bacterium Acidithiobacillus ferrooxidans2011

    • Author(s)
      菊本 愛生、金尾 忠芳、高田 潤、上村 一雄
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌コンベンションセンター(札幌)
    • Related Report
      2011 Research-status Report
  • [Presentation] Expression and characterization of a diheme cytochrome c encoded by gene cycA2 from the acidophilic Acidithiobacillus ferrooxidans as a recombinant protein in Escherichia coli2011

    • Author(s)
      田崎 翔悟、菊本 愛生、金尾 忠芳、上村 一雄
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌コンベンションセンター(札幌)
    • Related Report
      2011 Research-status Report
  • [Presentation] Acidithiobacillus ferrooxidansのチオ硫酸酸化に関与する遺伝子の同定2011

    • Author(s)
      菊本 愛生、金尾 忠芳、高田 潤、上村 一雄
    • Organizer
      日本農芸化学会中四国支部第30回講演会
    • Place of Presentation
      岡山大学(岡山)
    • Related Report
      2011 Research-status Report
  • [Presentation] Acidithiobacillus ferrooxidansのチオ硫酸デヒドロゲナーゼの構造と機能2011

    • Author(s)
      野上 翔平、菊本 愛生、金尾 忠芳、高田 潤、上村 一雄
    • Organizer
      平成23年度日本農芸化学会西日本支部・中四国支部合同大会
    • Place of Presentation
      宮崎大学(宮崎)
    • Related Report
      2011 Research-status Report
  • [Presentation] 酸性環境からの硫黄酸化能に優れた微生物の分離と培養

    • Author(s)
      石故明衣子,上村一雄,金尾忠芳
    • Organizer
      日本農芸化学会関西・中四国・西日本支部
    • Place of Presentation
      県立広島大学 広島キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 微生物の硫黄代謝酵素研究の新展開

    • Author(s)
      金尾忠芳,上村一雄
    • Organizer
      第65回日本生物工学会大会
    • Place of Presentation
      広島国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] 中等度酸性鉱山廃水中の微生物叢の解析

    • Author(s)
      王 揚,安田貴志,金尾忠芳
    • Organizer
      日本農芸化学会中四国支部第38回講演会
    • Place of Presentation
      香川大学農学部
    • Related Report
      2013 Annual Research Report
  • [Presentation] 海洋性硫黄酸化細菌Acidithiobacillus thiooxidans SH株の硫黄代謝

    • Author(s)
      金尾忠芳,梶谷泰之,上村一雄
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] Bioleaching and treatment of acid mine drainmage -Sustainable metal recovery system-

    • Author(s)
      上村 一雄
    • Organizer
      Seminar on Sustainable waste Management(招待講演)
    • Place of Presentation
      チッタゴン大学(バングラデシュ)
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.okayama-u.ac.jp/user/agr/profile/nougaku01_7.html

    • Related Report
      2013 Final Research Report
  • [Remarks] 微生物機能学ユニットURL

    • URL

      http://www.okayama-u.ac.jp/user/agr/profile/nougaku01_7.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 微生物機能学ユニットURL

    • URL

      http://www.okayama-u.ac.jp/user/agr/profile/nougaku01_7.html

    • Related Report
      2012 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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