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Synthesis of inner-core oligosaccharides of LPS and its protein conjugates for a definition of the epitope recognized by human antibodies

Research Project

Project/Area Number 23580474
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionTottori University

Principal Investigator

ICHIYANAGI Tsuyoshi  鳥取大学, 農学部, 准教授 (00302240)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordslipopolysaccharide / lipooligosaccharide / Kdo / conjugate / synthesis / 糖鎖ライブラリ合成 / グラム陰性菌 / 複合糖脂質 / リポ多糖 / 糖鎖ワクチン / 内部コア糖鎖 / Conjugate / LPS / LOS
Research Abstract

In this research, we successively achieved the synthesis of 9 type oligosaccharides, which are partial inner-core oligosaccarides of lipopolysaccharide/lipooligosaccharide produced by pathogenic gram-negative bacteria. And synthesized oligosaccharides were labeled with biotin and also conjugated to human serum albumin. These conjugates will be utilized for the definition of the epitope recognized by human antibody and the development of carbohydrate vaccine against pathogenic gram-negative bacterial infection.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (36 results)

All 2014 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 6 results) Presentation (27 results) Remarks (1 results)

  • [Journal Article] Synthesis of 4,5-disubstituted-3-deoxy-D-manno-oct ulosonic acid (Kdo) derivatives2014

    • Author(s)
      Ruiqin Yi, Atsushi Ogaki, Mayumi Fukunaga, Hiromitsu Nakajima, Tsuyoshi Ichiyanagi
    • Journal Title

      Tetrahedron

      Volume: 70 Issue: 23 Pages: 3675-3682

    • DOI

      10.1016/j.tet.2014.04.024

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Recent Synthetic Study of Inner-Core Saccharide of Lipopolysaccharides and Lipooligosaccharides2014

    • Author(s)
      Tsuyoshi Ichiyanagi
    • Journal Title

      Trends in Glycoscience and Glycotechnology

      Volume: 26 Issue: 149 Pages: 77-78

    • DOI

      10.4052/tigg.26.77

    • NAID

      130004841386

    • ISSN
      0915-7352, 1883-2113
    • Related Report
      2013 Final Research Report
  • [Journal Article] Recent synthetic study of inner-core saccharide of lipopolysaccharides and lipooligosaccharides2013

    • Author(s)
      Tsuyoshi Ichiyanagi
    • Journal Title

      Trends in Glycoscience and Glycotechnology

      Volume: 25

    • NAID

      130004841386

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Glycosylation Using an Organogold Complex as Promoter2012

    • Author(s)
      Tsuyoshi Ichiyanagi
    • Journal Title

      Trends in Glycoscience and Glycotechnology

      Volume: 24 Issue: 137 Pages: 132-133

    • DOI

      10.4052/tigg.24.132

    • NAID

      10030976076

    • ISSN
      0915-7352, 1883-2113
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Journal Article] Western Blot Analysis of Neisserial Lipooligosaccharide at the Lower Femtomole Level with Normal Human Sera2012

    • Author(s)
      Ryohei Yamasaki, Alice Ikezaki, Masaaki Seo, Tsuyoshi Ichiyanagi, Ichie Aoto
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 76 Issue: 2 Pages: 294-298

    • DOI

      10.1271/bbb.110664

    • NAID

      10030399750

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Western blot analysis of lipooligosaccharide at the lower femtomole level with normal human sera2012

    • Author(s)
      Ryohei Yamasaki, Alice Ikezaki, Masaaki Seo, Tsuyoshi Ichiyanagi, Ichie Aoto
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 76 Pages: 294-298

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] A new Kdo derivative for the synthesis of an inner-core disaccharide of lipopolysaccharides and lopooligosaccharides2011

    • Author(s)
      Tsuyoshi Ichiyanagi, Mayumi Fukunaga, Ryoya Tagashira, Shie Hayashi, Masato Nanjo, Ryohei Yamasaki
    • Journal Title

      Tetrahedron

      Volume: 67 Issue: 33 Pages: 5964-5971

    • DOI

      10.1016/j.tet.2011.06.039

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of D-Galacturonic Acid on Hydration of Cementitious Materials2011

    • Author(s)
      Cleopatra Panganayi, Hidehiko Ogata, Kunio Hattori, Tsuyoshi Ichiyanagi
    • Journal Title

      Journal of Materials in Civil Engineering.

      Volume: 23 Pages: 1596-1601

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 分子内グリコシド結合形成反応を利用するKdo2糖の合成研究2014

    • Author(s)
      田坂瑞葵,門脇拓哉,蟻瑞欽,一柳剛
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田キャンパス
    • Year and Date
      2014-03-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Synthesis of 4,5-di-O-substituted-3- deoxy-D-manno-octulosonic acid (Kdo) derivatives2013

    • Author(s)
      Ruiqin Yi, Mai Shimada, Tsuyoshi Ichiyanagi
    • Organizer
      第32回日本糖質学会年会
    • Place of Presentation
      大阪国際センター
    • Year and Date
      2013-08-07
    • Related Report
      2013 Final Research Report
  • [Presentation] 共通の中間体を用いた2-8結合を持つKdo2糖の合成研究2013

    • Author(s)
      庄野結大,大柿敦嗣,門脇拓哉,一柳剛
    • Organizer
      第32回日本糖質学会年会
    • Place of Presentation
      大阪国際センター
    • Year and Date
      2013-08-06
    • Related Report
      2013 Final Research Report
  • [Presentation] Synthetic study of Kdo derivatives for branched inner-core oligosaccharides of LPS/LOS2013

    • Author(s)
      Tsuyoshi Ichiyanagi
    • Organizer
      KoRS-CB Kolloquium
    • Place of Presentation
      Universtät Konstanz, Germany
    • Year and Date
      2013-07-15
    • Related Report
      2013 Final Research Report
  • [Presentation] LPS/LOSの酸性内部コア糖鎖の合成研究2013

    • Author(s)
      一柳剛,庄野結大,蟻瑞欽,島田麻衣,大柿敦嗣,門脇拓也
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学
    • Year and Date
      2013-03-26
    • Related Report
      2013 Final Research Report
  • [Presentation] SYNTHESIS OF GLYCOCONJUGATE CONTAINING IN THE INNER-CORE OLIGOSACCHARIDES EXPRESSED IN LPS/LOS2013

    • Author(s)
      Tsuyoshi Ichiyanagi, Mai Shimada, Ruiqin Yi
    • Organizer
      17^<th> European Carbohydrate Symposium
    • Place of Presentation
      Tel-Aviv, Israel
    • Related Report
      2013 Final Research Report
  • [Presentation] Synthesis of 4,5-di-O-substituted-3-deoxy-D-manno-octulosonic acid (Kdo) derivatives2013

    • Author(s)
      Ruiqin Yi, Mai Shimada, Hiromitsu Nakajima, Tsuyoshi Ichiyanagi
    • Organizer
      the International conference of 5th Asian communication of Glycoscience and Glycotechnology
    • Place of Presentation
      Khon Kean University, Thai
    • Related Report
      2013 Annual Research Report
  • [Presentation] Syntheis of glycoconjugate containing in the inner-cor oligosaccharides expressed in LPS/LOS2013

    • Author(s)
      Tsuyoshi Ichiyanagi, Mai Shimada, Ruiqin Yi
    • Organizer
      17th European Carbohydrate Symposium
    • Place of Presentation
      Tel-Aviv, Israel
    • Related Report
      2013 Annual Research Report
  • [Presentation] SYNTHESIS OF GLYCOCONJUGATE CONTAINING IN THE INNER-CORE OLIGOSACCHARIDES EXPRESSED IN LPS/LOS2013

    • Author(s)
      Tsuyoshi Ichiyanagi, Mai Shimada, Ruiqin Yi
    • Organizer
      17th European Carbohydrate Symposium
    • Place of Presentation
      Tel-Aviv, Israel
    • Related Report
      2012 Research-status Report
  • [Presentation] Synthesis of dimeric Kdo by using a common Kdo intermediate2012

    • Author(s)
      Keita Shono, Atsushi Ogaki, Mai Shimada, Tsuyoshi Ichiyanagi
    • Organizer
      26^<th> International Carbohydrate Symposium (ICS2012)
    • Place of Presentation
      Madrid, Spain
    • Year and Date
      2012-07-28
    • Related Report
      2013 Final Research Report
  • [Presentation] Synthesis of glycoconjugate precursors containing in acidic core oligosaccharide expressed in LPS/LOS2012

    • Author(s)
      Mai Shimada, Mayumi Fukunaga, Tsuyoshi Ichiyanagi
    • Organizer
      26^<th> International Carbohydrate Symposium (ICS2012)
    • Place of Presentation
      Madrid, Spain
    • Year and Date
      2012-07-28
    • Related Report
      2013 Final Research Report
  • [Presentation] コンドロイチン硫酸の糖鎖構造が決定する細胞分化の方向性2012

    • Author(s)
      保坂善真,田村純一,一柳剛,上原正人
    • Organizer
      第153回日本獣医学学術集会
    • Place of Presentation
      大宮ソニックシティ
    • Year and Date
      2012-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] LPS/LOSに存在するKdo2糖の合成:Kdo供与体の反応性2012

    • Author(s)
      一柳剛,大柿敦嗣,Yi Ruiqin,庄野結大,島田麻衣
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-24
    • Related Report
      2013 Final Research Report
  • [Presentation] 共通の中間体を用いたLPSに含まれるKdo2糖[Kdo(2-8)Kdo]の合成2012

    • Author(s)
      一柳剛,庄野結大,大柿敦嗣,島田麻衣
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-24
    • Related Report
      2013 Final Research Report
  • [Presentation] LPS/LOSの分岐酸性コア3糖コンジュゲート前駆体の合成2012

    • Author(s)
      一柳剛,島田麻衣,福永真弓,山崎良平
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-24
    • Related Report
      2013 Final Research Report
  • [Presentation] LPS/LOSに存在するKdo2糖の合成:Kdo供与体の反応性2012

    • Author(s)
      一柳剛, 大柿敦嗣, Yi Ruiqin, 庄野結大, 島田麻衣
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学
    • Related Report
      2011 Research-status Report
  • [Presentation] 共通の中間体を用いたLPSに含まれるKdo2糖[Kdo(2-8)Kdo]の合成2012

    • Author(s)
      一柳剛, 庄野結大, 大柿敦嗣, 島田麻衣
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学
    • Related Report
      2011 Research-status Report
  • [Presentation] LPS/LOS の分岐酸性コア3 糖コンジュゲート前駆体の合成2012

    • Author(s)
      一柳剛, 島田麻衣, 福永真弓, 山崎良平
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学
    • Related Report
      2011 Research-status Report
  • [Presentation] LOS/LPSの内部コア糖鎖合成に適したKdo誘導体合成2011

    • Author(s)
      一柳剛,福永真弓,田頭亮哉,山崎良平
    • Organizer
      日本糖質学会第30回年会
    • Place of Presentation
      新潟県長岡市(ハイブ長岡)
    • Year and Date
      2011-07-13
    • Related Report
      2013 Final Research Report
  • [Presentation] LOS/LPSの内部コア糖鎖合成に適したKdo誘導体合成2011

    • Author(s)
      一柳剛, 福永真弓, 田頭亮哉, 山崎良平
    • Organizer
      日本糖質学会第30回年会
    • Place of Presentation
      新潟県長岡市(ハイブ長岡)
    • Related Report
      2011 Research-status Report
  • [Presentation] 分子内グリコシド結合形成反応を利用するKdo2糖の合成研究

    • Author(s)
      田坂 瑞葵、門脇 拓哉、蟻 瑞欽、一柳 剛
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学、神奈川県川崎市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 共通の中間体を用いた2-8結合を持つKdo2糖の合成研究

    • Author(s)
      庄野 結大、大柿 敦嗣、門脇 拓哉、一柳 剛
    • Organizer
      第32回日本糖質学会年会
    • Place of Presentation
      大阪国際センター
    • Related Report
      2013 Annual Research Report
  • [Presentation] Synthesis of 4,5-di-O-substituted-3-deoxy-D-manno-octulosonic acid (Kdo) derivatives

    • Author(s)
      Ruiqin Yi, Mai Shimada, Tsuyoshi Ichiyanagi
    • Organizer
      第32回日本糖質学会年会
    • Place of Presentation
      大阪国際センター
    • Related Report
      2013 Annual Research Report
  • [Presentation] Synthetic study of Kdo derivatives for branched inner-core oligosaccharides of LPS/LOS

    • Author(s)
      Tsuyoshi Ichiyanagi
    • Organizer
      KoRS-CB Kolloquium
    • Place of Presentation
      Universtate Konstanz, Germany
    • Related Report
      2013 Annual Research Report
  • [Presentation] LPS/LOS の酸性内部コア糖鎖の合成研究

    • Author(s)
      一柳剛、庄野結大、蟻瑞欽、島田麻衣、大柿敦嗣、門脇拓也
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学
    • Related Report
      2012 Research-status Report
  • [Presentation] Synthesis of dimeric Kdo by using a common Kdo intermediate

    • Author(s)
      Keita Shono, Atsushi Ogaki, Mai Shimada, Tsuyoshi Ichiyanagi
    • Organizer
      26th International Carbohydrate Symposium (ICS2012)
    • Place of Presentation
      Madrid, Spain
    • Related Report
      2012 Research-status Report
  • [Presentation] Synthesis of glycoconjugate precursors containing in acidic core oligosaccharide expressed in LPS/LOS

    • Author(s)
      Mai Shimada, Mayumi Fukunaga, Tsuyoshi Ichiyanagi
    • Organizer
      26th International Carbohydrate Symposium (ICS2012),
    • Place of Presentation
      Madrid, Spain
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://staff.muses.tottori-u.ac.jp/yanagi/

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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