Synthesis of inner-core oligosaccharides of LPS and its protein conjugates for a definition of the epitope recognized by human antibodies
Project/Area Number |
23580474
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied molecular and cellular biology
|
Research Institution | Tottori University |
Principal Investigator |
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | lipopolysaccharide / lipooligosaccharide / Kdo / conjugate / synthesis / 糖鎖ライブラリ合成 / グラム陰性菌 / 複合糖脂質 / リポ多糖 / 糖鎖ワクチン / 内部コア糖鎖 / Conjugate / LPS / LOS |
Research Abstract |
In this research, we successively achieved the synthesis of 9 type oligosaccharides, which are partial inner-core oligosaccarides of lipopolysaccharide/lipooligosaccharide produced by pathogenic gram-negative bacteria. And synthesized oligosaccharides were labeled with biotin and also conjugated to human serum albumin. These conjugates will be utilized for the definition of the epitope recognized by human antibody and the development of carbohydrate vaccine against pathogenic gram-negative bacterial infection.
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Report
(4 results)
Research Products
(36 results)