Visualization and cleavage of DNA using fluorescence and ultrasonication

• Project/Area Number: 23590043
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Kyushu University
• Principal Investigator: NAKAZONO Manabu 九州大学, 薬学研究科(研究院), 助教 (90336074)
• Co-Investigator(Renkei-kenkyūsha): NANBU Shinkoh 上智大学, 理工学部, 教授 (00249955)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 蛍光 / インドリルマレイミド / DNA / ＤＮＡ / 分子科学計算

Research Abstract

Various unsymmetric indolylmaleimides were synthesized and the optical property
was clarified. Some indolylmaleimides have large Stokes shifts and fluorescence emission maxima
wavelengths of more than 550 nm. The fluorescence of indolylmaleimides was measured in the presence of double strand DNA. The fluorescence intensities of indolylmaleimides having N-methyl quinoline increased in the presence of double strand DNA.

Research Products

• [Journal Article] Unsynmetric indolylmaleimides; Synthesis, photophysical properties and amyloid detection (2014)
  • Author(s): M. Nakazono, K. Obayashi, Y. Oshikawa, K. Tani, Y. Ando, S. Nanbu, R. Kuwano
  • Journal Title: Journal of Photochemistry and Photobiology A. Chemistry Volume: 289 Pages: 39-46
  • Peer Reviewed
• [Journal Article] Unsynmetric indolylmaleimides; Synthesis, photophysical properties and amyloid detection (2014)
  • Author(s): M. Nakazono, K. Obayashi, Y. Oshikawa, K. Tani, Y. Ando, S. Nanbu, R. Kuwano
  • Journal Title: Journal of photochemistry and photobiology A. Chemistry Volume: in press
  • Peer Reviewed
• [Journal Article] Theoretical study of photo-physical properties of indolylmaleimide derivatives, Physical Chemistry (2012)
  • Author(s): Z. Zheng, Y. Zhao, M. Nakazono, S. Nanbu
  • Journal Title: Chemical Physics Volume: 14 Pages: 3017-3024
  • Peer Reviewed
• [Journal Article] Photochemical dynamics of indolylmaleimide derivatives, Physical Chemistry (2012)
  • Author(s): T. Murakami, M. Nakazono, A. Kondorskiy, T. Ishida , S. Nanbu
  • Journal Title: Chemical Physics Volume: 14 Pages: 11546-11555
  • Peer Reviewed
• [Journal Article] Photochemical dynamics of indolylmaleimide derivatives (2012)
  • Author(s): T. Murakami, M. Nakazono, A. Kondorskiy, T. Ishida, S. Nanbu
  • Journal Title: Physical Chemistry Chemical Physics Volume: 14 Issue: 32 Pages: 11546-11555
  • DOI: 10.1039/c2cp41269a
  • Peer Reviewed
• [Journal Article] Theoretical study of photo-physical properties of indolylmaleimide derivatives (2012)
  • Author(s): ZiLong Zheng, Yi Zhao, Manabu Nakazono, Shinkoh Nanbu
  • Journal Title: J.Phys.Chem.A Volume: 14 Issue: 9 Pages: 3017-3024
  • DOI: 10.1039/c2cp22946c
  • Peer Reviewed
• [Presentation] 様々なアミロイドを検出する化合物の開発 (2014)
  • Author(s): 中園 学、大林光念、佐々本一美、冨吉勝美、末永元輝、安東由喜雄
  • Organizer: 日本薬学会 134年会
  • Place of Presentation: 熊本
• [Presentation] 新規化学発光化合物の開発 (2013)
  • Author(s): 中園学
  • Organizer: 第26回バイオメディカル分析科学シンポジウム
  • Place of Presentation: 東京
• [Presentation] 非対称型インドリルマレイミド(合成、蛍光特性及びアミロイド検出) (2013)
  • Author(s): 中園学、大林光念、押川祐二、多仁一司、末永元輝、安東由喜雄、南部伸孝、桑野良一
  • Organizer: 第26回バイオメディカル分析科学シンポジウム
  • Place of Presentation: 東京
• [Presentation] Unsymmetric Indolylmaleimides (Synthesis, photophysical properties and amyloid detection) (2013)
  • Author(s): M. Nakazono, K. Obayashi, Y. Oshikawa, K. Tani, G. Suenaga, Y. Ando, S. Nanbu, R. Kuwano
  • Organizer: The Twelfth Asian Conference on Analytical Sciences
  • Place of Presentation: Fukuoka, Japan
• [Presentation] 非対称型インドリルマレイミド（合成、蛍光特性及びアミロイド検出） (2013)
  • Author(s): 中園 学、大林光念、押川祐二、多仁一司、末永元輝、安東由喜雄
  • Organizer: 第26回バイオメディカル分析科学シンポジウム
  • Place of Presentation: 東京
• [Presentation] Unsymmetric Indolylmaleimides (Synthesis, photophysical properties and amyloid detection) (2013)
  • Author(s): M. Nakazono, K. Obayashi, Y. Oshikawa, K. Tani, G. Suenaga, Y. Ando, S. Nanbu, R. Kuwano
  • Organizer: The Twelfth Asian Conference on Analytical Sciences
  • Place of Presentation: 福岡
• [Presentation] 発光及びフラーレン化合物の実験と理論 (2012)
  • Author(s): 中園学
  • Organizer: シンポジウム「新生命科学分野開拓とスーパーコンピュータ「京」」
  • Place of Presentation: 福岡
• [Presentation] Fluorescence property of unsymmetric indolylmaleimide derivatives (2012)
  • Author(s): M. Nakazono, S. Nanbu, R. Kuwano
  • Organizer: SCS-2012 Fall Meeting
  • Place of Presentation: Zürich, Switzerland
• [Presentation] Fluorescence property of unsymmetric indolylmaleimide derivatives (2012)
  • Author(s): M. Nakazono, S. Nanbu, R. Kuwano
  • Organizer: Swiss Chemical Soceity-2012 Fall Meeting
  • Place of Presentation: スイス（チューリヒ）
• [Presentation] 様々なアミロイドを検出する化合物の開発
  • Author(s): 中園学、大林光念、佐々本一美、冨吉勝美、末永元輝、安東由喜雄
  • Organizer: 日本薬学会134年会
  • Place of Presentation: 熊本
• [Presentation] 新たな特性を有した化学発光化合物の開発
  • Author(s): 中園 学
  • Organizer: 生物発光化学発光研究会(招待講演)
  • Place of Presentation: 長崎