• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mechanisms of neuronal-glial-vascular interactions in the retina and the application for development of anti-glaucoma drugs

Research Project

Project/Area Number 23590112
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biological pharmacy
Research InstitutionKitasato University

Principal Investigator

NAKAHARA Tsutomu  北里大学, 薬学部, 准教授 (10296519)

Co-Investigator(Kenkyū-buntansha) MORI Asami  北里大学, 薬学部, 助教 (80453504)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords薬理学 / 血管生物学 / 網膜神経 / グリア細胞 / 網膜血管 / 神経節細胞
Research Abstract

This study aimed to clarify the mechanisms of neuronal-glial-vascular interactions in the retina using a rat model of retinal degeneration induced by an intravitreal injection of NMDA. In this model, loss of neuronal cells, accumulation of leukocytes, activation of microglia, and vascular injury were observed after NMDA treatment. Neuronal cell loss and microglia activation preceded vascular injury following NMDA treatment. Inhibitors of macrophage migration inhibitory factor (MIF) and mammalian target of rapamycin (mTOR) attenuated these NMDA-induced responses. Therefore, compounds acting on these molecules may have the potential to correct the neuronal-glial-vascular interaction and ultimately be used for the treatment of glaucoma.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (23 results)

All 2014 2013 2012 2011 Other

All Journal Article (12 results) (of which Peer Reviewed: 11 results,  Open Access: 1 results) Presentation (10 results) Remarks (1 results)

  • [Journal Article] Rapamycin prevents N-methyl-D-aspartate-induced retinal damage through an ERK-dependent mechanism in rats2014

    • Author(s)
      Ichikawa A, Nakahara T, Kurauchi Y, Mori A, Sakamoto K, Ishii K
    • Journal Title

      J Neurosci Res

      Volume: 92 Issue: 6 Pages: 692

    • DOI

      10.1002/jnr.23358

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Differential effects of LY294002 and wortmannin on neurons and vascular endothelial cells in the rat retina2013

    • Author(s)
      Ueda K, Nakahara T, Akanuma K, Mori A, Sakamoto K, Ishii K
    • Journal Title

      Pharmacol Rep

      Volume: 65(4) Pages: 854-862

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Journal Article] ISO-1, a macrophage migration inhibitory factor antagonist, prevents N-methyl-d-aspartate-induced retinal damage2013

    • Author(s)
      Naruoka T, Nakahara T, Tsuda Y, Kurauchi Y, Mori A, Sakamoto K, Nishihira J, Ishii K
    • Journal Title

      Eur J Pharmacol

      Volume: 718 Issue: 1-3 Pages: 138

    • DOI

      10.1016/j.ejphar.2013.08.041

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Neurovascular Interactions in the Retina: Physiological and Pathological Roles2013

    • Author(s)
      Nakahara T, Mori A, Kurauchi Y, Sakamoto K, Ishii K
    • Journal Title

      Journal of Pharmacological Sciences

      Volume: 123 Issue: 2 Pages: 79-84

    • DOI

      10.1254/jphs.13R03CP

    • NAID

      130003382600

    • ISSN
      1347-8613, 1347-8648
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protective effects of TGF-β inhibitors in a rat model of NMDA-induced retinal degeneration2013

    • Author(s)
      Ueda K, Nakahara T, Mori A, Sakamoto K, Ishii K
    • Journal Title

      Eur J Pharmacol

      Volume: 699 Issue: 1-3 Pages: 188

    • DOI

      10.1016/j.ejphar.2012.11.054

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of Nafamostat on <i>N</i>-Methyl-<small>D</small>-aspartate-Induced Retinal Neuronal and Capillary Degeneration in Rats2012

    • Author(s)
      Tsuda Y, Nakahara T, Ueda K, Mori A, Sakamoto K, Ishii K
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 35 Issue: 12 Pages: 2209-2213

    • DOI

      10.1248/bpb.b12-00644

    • NAID

      130002480514

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Protective effects of the β<sub>3</sub>-adrenoceptor agonist CL316243 against N-methyl-D-aspartate-induced retinal neurotoxicity2012

    • Author(s)
      Oikawa F, Nakahara T, Akanuma K, Ueda K, Mori A, Sakamoto K, Ishii K
    • Journal Title

      Naunyn Schmiedebergs Arch Pharmacol

      Volume: 385 Issue: 11 Pages: 1077

    • DOI

      10.1007/s00210-012-0796-1

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Role of β <sub>3</sub>-adrenoceptors in regulation of retinal vascular tone in rats2011

    • Author(s)
      Mori A, Nakahara T, Sakamoto K, Ishii K
    • Journal Title

      Naunyn Schmiedebergs Arch Pharmacol

      Volume: 384 Issue: 6 Pages: 603

    • DOI

      10.1007/s00210-011-0682-2

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Noradrenaline contracts rat retinal arterioles via stimulation of α1A- and α1D-adrenoceptors2011

    • Author(s)
      Mori A, Hanada M, Sakamoto K, Nakahara T, Ishii K
    • Journal Title

      Eur J Pharmacol

      Volume: 673(1-3) Pages: 65-69

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Role of β3-adrenoceptors in regulation of retinal vascular tone in rats2011

    • Author(s)
      Mori A, Nakahara T, Sakamoto K, Ishii K
    • Journal Title

      Naunyn-Schmiedeberg's Arch Pharmacol

      Volume: 384(6) Pages: 603-608

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Vasodilation of retinal arterioles induced by activation of BKCa channels is attenuated in diabetic rats2011

    • Author(s)
      Mori A, Suzuki S, Sakamoto K, Nakahara T, Ishii K
    • Journal Title

      Eur J Pharmacol

      Volume: 669(1-3) Pages: 94-99

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Small molecule cyclin-dependent kinase inhibitors protect against neuronal cell death in the ischemic-reperfused rat retina2011

    • Author(s)
      Sakamoto K, Ohki K, Saito M, Nakahara T, Ishii K
    • Journal Title

      J Ocul Pharmacol Ther

      Volume: 27(5) Pages: 419-425

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] ラット網膜においてNMDAで誘発される神経傷害および血管傷害に対する成熟度の影響2013

    • Author(s)
      浅野大樹、浅見康之、森麻美、坂本謙司、中原努、石井邦雄
    • Organizer
      第15回応用薬理シンポジウム年会
    • Place of Presentation
      東京:コクヨホール
    • Year and Date
      2013-09-28
    • Related Report
      2013 Final Research Report
  • [Presentation] ラットにおけるTGF-β阻害薬のNMDA誘発網膜神経/血管傷害に対する抑制効果2012

    • Author(s)
      中原努、植田香、森麻美、坂本謙司、石井邦雄
    • Organizer
      第32回日本眼薬理学会学術集会
    • Place of Presentation
      滋賀:ピアザ淡海
    • Year and Date
      2012-09-16
    • Related Report
      2013 Final Research Report
  • [Presentation] ラットNMDA誘発網膜神経傷害に対するマクロファージ遊走阻止因子抑制薬ISO-1の効果2012

    • Author(s)
      成岡妙子、中原努、倉内祐樹、森麻美、坂本謙司、石井邦雄
    • Organizer
      第126回日本薬理学会関東部会
    • Place of Presentation
      東京:北里大学薬学部
    • Year and Date
      2012-07-14
    • Related Report
      2013 Final Research Report
  • [Presentation] 幼若期および成熟期ラットにおいて観察される視神経傷害に伴う網膜血管傷害2012

    • Author(s)
      浅見康之、中原努、倉内祐樹、森麻美、坂本謙司、石井邦雄
    • Organizer
      第126回日本薬理学会関東部会
    • Place of Presentation
      東京:北里大学薬学部
    • Year and Date
      2012-07-14
    • Related Report
      2013 Final Research Report
  • [Presentation] アドレナリン受容体刺激薬CL316243の網膜神経保護効果2012

    • Author(s)
      中原努、赤沼かおり、及川風花、森麻美、坂本謙司、石井邦雄
    • Organizer
      第85回日本薬理学会年会
    • Place of Presentation
      京都:国立京都国際会館
    • Year and Date
      2012-03-14
    • Related Report
      2013 Final Research Report
  • [Presentation] ノルアドレナリンはα1A- および α1D-アドレナリン受容体を介してラット網膜細動脈を収縮させる2012

    • Author(s)
      森麻美、花田真幸、坂本謙司、中原努、石井邦雄
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      北海道大学(札幌)
    • Related Report
      2011 Research-status Report
  • [Presentation] カプサイシンはオピオイド受容体の刺激を介してラット網膜におけるNMDA誘発神経細胞死を抑制する2012

    • Author(s)
      坂本謙司、黒木大陽、関谷春菜、渡辺彰宏、森麻美、中原努、石井邦雄
    • Organizer
      第85回日本薬理学会年会
    • Place of Presentation
      国立京都国際会館(京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] β3アドレナリン受容体刺激薬CL316243の網膜神経保護効果2012

    • Author(s)
      中原努、赤沼かおり、及川風花、森麻美、坂本謙司、石井邦雄
    • Organizer
      第85回日本薬理学会年会
    • Place of Presentation
      国立京都国際会館(京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] ラット網膜において NMDA で誘発される神経傷害および血管傷害に対する成熟度の影響

    • Author(s)
      浅野 大樹,浅見 康之,森 麻美,坂本 謙司,中原 努,石井 邦雄
    • Organizer
      第 15 回応用薬理シンポジウム年会
    • Place of Presentation
      東京 (会場:コクヨホール)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ラット NMDA 誘発網膜神経傷害に対するマクロファージ遊走阻止因子抑制薬 ISO-1 の効果

    • Author(s)
      成岡 妙子,中原 努,倉内 祐樹,森 麻美,坂本 謙司,石井 邦雄
    • Organizer
      第126回日本薬理学会関東部会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.pharm.kitasato-u.ac.jp/molpharm/molpharm/TOP.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi