The action of IL-24 involving the exacerbation of pneumonia in RSV-infected mice exposed perinatally to a brominated flame retardant.

• Project/Area Number: 23590161
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental pharmacy
• Research Institution: Kyushu University of Health and Welfare
• Principal Investigator: WATANABE Wataru 九州保健福祉大学, 薬学部, 教授 (50399218)
• Co-Investigator(Kenkyū-buntansha): KUROKAWA Masahiko 九州保健福祉大学, 大学院医療薬学研究科, 教授 (80186527) ; YOSHIDA Hiroki 九州保健福祉大学, 大学院医療薬学研究科, 講師 (90469411)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: RSV / IL-24 / 臭素化難燃剤 / TBBPA / ウイルス / 免疫学 / 有害化学物質 / RSウイルス

Research Abstract

To reveal effects of perinatal exposure to a brominated flame retardant TBBPA on the exacerbation of RSV-induced pneumonia, mechanism of action of IL-24 on RSV infection was investigated.
By immune histopathological analysis, increase of the expression of IL-24 was confirmed in the lung tissues of RSV-infected mice exposed perinatally to TBBPA. The expression of IL-24 was also found in RA264.7 cells by real-time RT-PCR and western blotting in vitro. Although correlation between increase of IL-24 and decrease of a proinflammatory cytokine TNF-alpha was observed in the RSV-infected cells, direct interaction of them could not be revealed due to the failure of RNAi against IL-24 gene. Increase of IL-24 induced due to TBBPA-exposure might affect the production of proinflammatory cytokine from macrophages, should result in disorder of immune system responding to RSV infection.

Research Products

• [Journal Article] Perinatal exposure to insecticide methamidophos suppressed production of proinflammatory cytokines responding to virus infection in lung tissues in mice. (2013)
  • Author(s): Watanabe,W., Yoshida, H., Hirose, A., Akashi, T., Takeshita, T., Kuroki, N., Shibata, A., Hongo, S., Hashiguchi, S., Konno, K., Kurokawa, M.
  • Journal Title: BioMed. Res. Int. Volume: 0 Pages: 151807-151814
  • DOI: 10.1155/2013/151807
  • Peer Reviewed
• [Journal Article] Effect of Brazilian propolis on exacerbation of respiratory syncytial virus infection in mice exposed to tetrabromobisphenol A, a brominated flame retardant. (2013)
  • Author(s): Takeshita, T., Watanabe, W., Toyama, S., Hayashi, Y., Honda, S., Sakamoto, S., Matsuoka, S., Yoshida, H., Takeda, S., Hidaka, M., Tsutsumi, S., Yasukawa, K., Park, Y.K., Kurokawa, M.
  • Journal Title: Evid.-Based Compl. Alt. Med. Volume: 0 Pages: 698206-698212
  • DOI: 10.1155/2013/698206
  • Peer Reviewed
• [Journal Article] Effect of oral administration of probiotics from Mongolian dairy products on Th1 immune response in mice (2013)
  • Author(s): Takeda, S., Kawahara, S., Hidaka, M., Yoshida, H., Watanabe, W., Takeshita, M., Kikuchi, Y., Bumbein, D., Muguruma, M., Kurokawa, M
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 77 Pages: 1372-1378
  • Peer Reviewed
• [Journal Article] Citrus flavonoid naringenin inhibits TLR2 expression in adipocytes (2013)
  • Author(s): Yoshida, H., Watanabe, W., Oomagari, H., Tsuruta, E., Shida, M., Kurokawa, M
  • Journal Title: J. Nutr. Biochem Volume: 24 Pages: 1276-1284
  • Peer Reviewed
• [Journal Article] Corticosteroids plus long-acting beta2-agonists prevent double-stranded RNA-induced upregulation of B7-H1 on airway epithelium (2013)
  • Author(s): Kan-o, K., Matsumoto, K., Inoue, H., Fukuyama, S., Asai, Y., Watanabe, W., Kurokawa, M., Araya, J., Kuwano, K., Natanishi, Y
  • Journal Title: Int. Arch. Allergy Immunol Volume: 160 Pages: 27-36
  • Peer Reviewed
• [Journal Article] Effect of oral administration of probiotics from Mongolian dairy products on Th1 immune response in mice. (2013)
  • Author(s): Takeda, S., Kawahara, S., Hidaka, M., Yoshida, H., Watanabe, W., Takeshita, M., Kikuchi, Y., Bumbein, D., Muguruma, M., Kurokawa, M.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 77 Pages: 1372-1378
  • Peer Reviewed
• [Journal Article] Antiviral activity of diarylheptanoid stereoisomers against respiratory syncytial virus in vitro and in vivo. (2013)
  • Author(s): Konno, K., Miura, M., Toriyama, M., Motohashi, S., Sawamura, R., Watanabe, W., Yoshida, H., Kato, M., Yamamoto, R., Yasukawa, K., Kurokawa, M.
  • Journal Title: J. Nat. Med. Volume: -
  • Peer Reviewed
• [Journal Article] Citrus flavonoid naringenin inhibits TLR2 expression in adipocytes. (2013)
  • Author(s): Yoshida, H., Watanabe, W., Oomagari, H., Tsuruta, E., Shida, M., Kurokawa, M.
  • Journal Title: J. Nutr. Biochem. Volume: -
  • Peer Reviewed
• [Journal Article] Corticosteroids plus long-acting beta2-agonists prevent double-stranded RNA-induced upregulation of B7-H1 on airway epithelium. (2013)
  • Author(s): Kan-o, K., Matsumoto, K., Inoue, H., Fukuyama, S., Asai, Y., Watanabe, W., Kurokawa, M., Araya, J., Kuwano, K., Natanishi, Y.
  • Journal Title: Int. Arch. Allergy Immunol. Volume: 160 Pages: 27-36
  • Peer Reviewed
• [Journal Article] Antiviral activities of diarylheptanoids isolated from Alpinia officinarum against respiratory syncytial virus, poliovirus, measles virus and herpes simplex virus type 1 in vitro (2012)
  • Author(s): Konno, K., Sawamura, R., Sun, Y., Yasukawa, K., Shimizu, T., Watanabe, W., Kato, M., Yamamoto, R., Kurokawa, M
  • Journal Title: Nat. Prod. Commun Volume: 6 Pages: 1881-1884
  • Peer Reviewed
• [Journal Article] Efficacy of oral administration of heat-killed probiotics from Mongolian dairy products against influenza infection in mice : Alleviation of influenza infection by its immunomodulatory activity through intestinal immunity (2011)
  • Author(s): Takeda, S. Takeshita, M., Kikuchi, Y., Dashnyam, B., Kawahara, S., Yoshida, H., Watanabe, W., Muguruma, M., Kurokawa, M
  • Journal Title: Int. Immunopharmacol Volume: 11 Pages: 1976-1983
  • Peer Reviewed
• [Journal Article] Antiviral activities of diarylheptanoids isolated from Alpinia officinarum against respiratory syncytial virus, poliovirus, measles virus and herpes simplex virus type 1 in vitro. (2011)
  • Author(s): Konno, K., Sawamura, R., Sun, Y., Yasukawa, K., Shimizu, T., Watanabe, W., Kato, M., Yamamoto, R., Kurokawa, M.
  • Journal Title: Nat. Prod. Commun. Volume: 6 Pages: 1881-1884
  • Peer Reviewed
• [Journal Article] Efficacy of Korean traditional medicines against influenza virus infection in mice and their modes of anti-influenza virus action (2011)
  • Author(s): Shimizu, T., Sawamura, R., Kim, K-H., Kim, K-H., Lee, S-H., Lee, Y-S., Kim, T-G., Kai, H., Yoshida, H., Watanabe, W., Matsuno, K., Shiraki, K., Kurokawa, M.
  • Journal Title: J. Trad. Med. Volume: 28 Pages: 115-127
  • NAID: 130004495573
  • Peer Reviewed
• [Journal Article] Efficacy of oral administration of heat-killed probiotics from Mongolian dairy products against influenza infection in mice: Alleviation of influenza infection by its immunomodulatory activity through intestinal immunity. (2011)
  • Author(s): Takeda, S. Takeshita, M., Kikuchi, Y., Dashnyam, B., Kawahara, S., Yoshida, H., Watanabe, W., Muguruma, M., Kurokawa,
  • Journal Title: Int. Immunopharmacol. Volume: 11 Pages: 1976-1983
  • Peer Reviewed
• [Presentation] 多層型カーボンナノチューブ曝露によるRSウイルス感染免疫への影響 (2014)
  • Author(s): 橋口誠子、吉田裕樹、徳田健治、紺野克彦、明石敏、広瀬明彦、黒川昌彦、渡辺渡
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本、ポスター28pmS-064
  • Year and Date: 2014-02-28
• [Presentation] Perinatal exposure to an insecticide methamidophos affected the immune redponse to virus infection in the next generation of mice (2013)
  • Author(s): Wataru Watanabe, Hiroki Yoshida, Akihiko Hirose, Masahiko Kurokawa
  • Organizer: EUROTOX2013
  • Place of Presentation: Interlaken, Switzerland, poster P11-15
  • Year and Date: 2013-09-02
• [Presentation] 有機リン系殺虫剤メタミドホスの周産期曝露によるRSウイルス感染免疫への影響 (2013)
  • Author(s): 渡辺渡、吉田裕樹、広瀬明彦、黒木奈緒、本郷聡子、紺野克彦、黒川昌彦
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜、ポスター30pmE-205
  • Year and Date: 2013-03-30
• [Presentation] Effects of nanoparticles of titanium dioxide on the immune redponse to virus infection (2012)
  • Author(s): Wataru Watanabe, Hiroki Yoshida, Akihiko Hirose, Katsuhiko Konno, Masahiko Kurokawa
  • Organizer: EUROTOX2012
  • Place of Presentation: Stockholm, Sweden, poster P20-02
  • Year and Date: 2012-06-19
• [Presentation] 酸化チタンナノ粒子のマクロファージおよびマウスモデルにおけるRSウイルス感染免疫応答における影響 (2012)
  • Author(s): 渡辺渡,吉田裕樹,広瀬明彦, 紺野克彦, 山中沙代子, 黒木奈緒, 黒川昌彦
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
• [Presentation] Effects of the nanoparticles of titanium dioxide on the immune response to respiratory syncytial virus (RSV) infection in macrophages in vitro and mouse model in vivo (2011)
  • Author(s): Watanabe, W., Yoshida, H., Hirose, A., Konno, K., Kurokawa, M
  • Organizer: International Congress of Virology
  • Place of Presentation: Sapporo
• [Presentation] Efficacy of Brazilian propolis against herpes simplex virus type 1 infection in mice and their modes of anti-herpetic efficacies (2011)
  • Author(s): Yoshida1, H., Kai, H., Tsutsumi, S, Yasukawa, K., Matsuno, K., Watanabe, W., Shiraki, K., Kurokawa, M
  • Organizer: International Congress of Virology
  • Place of Presentation: Sapporo
• [Presentation] RSウイルス感染マウスモデルを用いた臭素化難燃物質の発達期免疫毒性の評価「発達期免疫毒性(developmental immunotoxicity)の評価方法」 (2011)
  • Author(s): 渡辺渡
  • Organizer: 第18回日本免疫毒性学会学術大会
  • Place of Presentation: 千葉
  • Invited
• [Presentation] Effects of the nanoparticles of titanium dioxide on the immune response to respiratory syncytial virus (RSV) infection in macrophages in vitro and mouse model in vivo. (2011)
  • Author(s): Watanabe, W., Yoshida, H., Hirose, A., Konno, K., Kurokawa, M
  • Organizer: International Congress of Virology 2011
  • Place of Presentation: Sapporo
• [Presentation] ウイルス感染マウスモデルを用いた臭素化難燃物質の発達期免疫毒性の評価，試験法ワークショップ「発達期免疫毒性（developmental immunotoxicity）の評価方法」 (2011)
  • Author(s): 渡辺 渡
  • Organizer: 第18回日本免疫毒性学会学術大会(招待講演)
  • Place of Presentation: 千葉
• [Presentation] 多層型カーボンナノチューブ曝露によるRSウイルス感染免疫への影響
  • Author(s): 橋口誠子、吉田裕樹、徳田健治、紺野克彦、明石 敏、広瀬明彦、黒川昌彦、渡辺 渡
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本（市民体育館）
• [Presentation] Perinatal exposure to an insecticide methamidophos affected the immune redponse to virus infection in the next generation of mice.
  • Author(s): Wataru Watanabe, Hiroki Yoshida, Akihiko Hirose, Masahiko Kurokawa
  • Organizer: EUROTOX2013
  • Place of Presentation: スイス（インターラーケン国際会議場）
• [Presentation] Effects of nanoparticles of titinium dioxide on the immune response to virus infection.
  • Author(s): Watanabe, W., Yoshida, H., Hirose, A., Konno, K., Kurokawa, M.
  • Organizer: EuroTox2012
  • Place of Presentation: Stockholm, Sweden
• [Presentation] 有機リン系殺虫剤メタミドホスの周産期暴露によるRSウイルス感染免疫への影響
  • Author(s): 渡辺 渡, 吉田裕樹，広瀬明彦，黒木奈緒，本郷聡子，柴田麻美, 紺野克彦, 黒川昌彦
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜
• [Book] 薬学領域の食品衛生化学 (2013)
  • Author(s): 渡辺渡(分担), 編集長澤一樹、川崎直人
  • Publisher: 廣川書店
• [Book] 薬学領域の生化学"第12章遺伝子工学の基礎" (2013)
  • Author(s): 黒川昌彦(分担), 編集伊藤晃
  • Publisher: 廣川書店
• [Book] 薬学領域の食品衛生化学 (2013)
  • Author(s): 川崎直人、田村悦臣、長岡寛明、長澤一樹、渡辺 渡
  • Total Pages: 248
  • Publisher: 廣川書店
• [Remarks] 研究室HP
  • URL: http://biochem-kuhw.org/
• [Remarks] 九州保健福祉大学薬学部生化学講座・微生物学研究室
  • URL: http://biochem-kuhw.org/
• [Remarks] 九州保健福祉大学薬学部薬学科 微生物学研究室ホームページ
  • URL: http://biochem-kuhw.org/