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Interaction of WNT family and TGF-beta family during myogenic differentiation of mesenchymal progenitor cells

Research Project

Project/Area Number 23590227
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKawasaki Medical School

Principal Investigator

NOHNO Tsutomu  川崎医科大学, 医学部, 教授 (20098619)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordsWNT / TGFβ / 骨格筋分化 / マイオスタチン / BMP4 / βカテニン / C2C12 / 骨格筋 / 筋分化 / miRNA / Smad1/5 / 筋芽細胞 / Wnt3a / Wnt4 / noggin / ウイルスベクター
Research Abstract

Several Wnt signaling components including Wnt4, Sfrp2, and Porcupine were induced after serum starvation during myogenic differentiation in C2C12 myoblast cell line, and Wnt4 was most effective to induce myogenic differentiation through suppression of beta-catenin signaling with Wnt3a, when overexpressed in C2C12 cells. Stable Wnt4-expressing sub-cell lines (W4-08) of C2C12 indicate decreased proliferation accompanying spontaneous differentiation into myotube cells with myosin heavy chain expression. BMP4 expression was up-regulated in W4-08 cells, as observed in C2C12 cells after serum starvation. BMP4 is acting to down-regulate myogenic differentiation, and noggin is effective to promote myogenic differentiation in the presence of Wnt3a and Wnt4, suggesting possible interaction between Wnt/beta-catenin and BMP/Smad pathways.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (13 results)

All 2013 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 3 results) Presentation (6 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Interaction of Wnt signaling with BMP/Smad signaling during the transition from cell proliferation to myogenic differentiation in mouse myoblast-derived cells2013

    • Author(s)
      Terada K,Misao S,Katase N,Nishimatsu S,Nohno T
    • Journal Title

      Int J Cell Biol 2013

      Volume: (Article ID 616294) Pages: 1-11

    • DOI

      10.1155/2013/616294

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] DKK3 (dickkopf 3 homolog (Xenopus laevis))2013

    • Author(s)
      Katase N, Nohno T
    • Journal Title

      Atlas Genet Cytogenet Oncol Haematol

      Volume: -

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genistein affects osteoblastic MC3T3-E1 cells through estrogen receptor and BMP-Smad signaling pathways2013

    • Author(s)
      Hinenoya H, Katsuyama H, Nohno T
    • Journal Title

      Kawasaki Med J

      Volume: 39 Pages: 21-31

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Canonical Wnt signaling is involved in switching from cell proliferation to myogenic differentiation of mouse myoblast cells2011

    • Author(s)
      Tanaka S,Terada K,Nohno T
    • Journal Title

      J Mol Signal

      Volume: 6 Pages: 1-12

    • DOI

      10.1186/1750-2187-6-12

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Importance of Wnt8 lipid modification during formation of the Spemann's organizer in early Xenopus embryos.2012

    • Author(s)
      成田知弘, 本村恵理子, 西松伸一郎, 坂井雅夫, 濃野 勉
    • Organizer
      第45回日本発生生物学会・第64回日本細胞生物学会合同大会
    • Place of Presentation
      神戸国際会議場(神戸)
    • Related Report
      2012 Research-status Report
  • [Presentation] Wnt signalling acts as a switch controlling myoblast cell proliferation and differentiation2011

    • Author(s)
      Tanaka S,Nishimatsu S,Ohsawa Y,Sunada Y,Nohno T
    • Organizer
      International Conference on Muscle Wasting 2011
    • Place of Presentation
      Centro Stefano Franscini,Monte Verita,Ascona,Switzeland
    • Year and Date
      2011-09-18
    • Related Report
      2013 Final Research Report
  • [Presentation] Wnt signaling during transition from cell proliferation to differentiation in myoblast cells2011

    • Author(s)
      Tanaka S,Nishimatsu S,Yamamura M,Nohno T
    • Organizer
      Wnt 2011
    • Place of Presentation
      Sproul Hall,Covel Commons,UCLA,CA,USA
    • Year and Date
      2011-07-01
    • Related Report
      2013 Final Research Report
  • [Presentation] Wnt signaling during transition from cell proliferation to differentiation in myoblast cells.2011

    • Author(s)
      Tanaka S, Nishimatsu S, Yamamura M, Nohno T
    • Organizer
      Wnt 2011
    • Place of Presentation
      Sproul Hall, Covel Commons, UCLA, CA, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] Wnt signalling acts as a switch controlling myoblast cell proliferation and differentiation.2011

    • Author(s)
      Tanaka S, Nishimatsu S, Ohsawa Y, Sunada Y, Nohno T
    • Organizer
      International Conference on Muscle Wasting 2011
    • Place of Presentation
      Centro Stefano Franscini, Monte Verita, Ascona, Switzeland
    • Related Report
      2011 Research-status Report
  • [Presentation] Wnt signaling during transition from cell proliferation to differentiation in C2C12 cells.2011

    • Author(s)
      Tanaka S, Terada K, Nohno T
    • Organizer
      MBSJ 2011(第34回日本分子生物学会年会)
    • Place of Presentation
      Pacifico Yokohama, Yokohama, Japan
    • Related Report
      2011 Research-status Report
  • [Book] DKK3,a mysterious tumor suppressor gene that possesses multiple functions in tumor progression. In "Tumor Suppressor Genes : Functions,Regulation and Health Effects"2013

    • Author(s)
      Katase N,Nohno T,Gunduz M
    • Publisher
      NOVA Science Publishers,Hauppauge,New York,USA
    • Related Report
      2013 Final Research Report
  • [Book] Tumor Suppressor Genes: Functions, Regulation and Health Effects2013

    • Author(s)
      Katase N, Nohno T, Gunduz M
    • Publisher
      NOVA Science Publishers
    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://www.kawasaki-m.ac.jp/molbiol/

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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