Analysis of LECT2 receptor and its signal transduction

• Project/Area Number: 23590275
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: SATOSHI Yamagoe 国立感染症研究所, 真菌部, 主任研究官 (00212283)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 生理活性 / シグナル伝達 / LECT2

Research Abstract

Accumulating evidences indicate that LECT2 is associated with several physiological phenomena, but its function is not fully understood. To make clear the function of LECT2, it is important to identify the LECT2 receptor. We performed expression cloning with a retroviral cDNA library constructed from a cell line that highly expressed the LECT2 receptor. Retrovirus infected cells binding LECT2-Fc fusion protein were enriched by cell sorting. The seven cell lines cloned from the enriched cells all had cDNAs derived from the same gene in integrated retrovirus vector. We checked the possibility that this protein was the LECT2 receptor by some experiments. Moreover, we analyzed the phosphoprotein involved in LECT2 signal transduction by western blotting with thirty different kinds of anti-phosphoprotein antibodies, identifying some possible phosphoproteins.

Research Products

• [Journal Article] LECT2 functions as a hepatokine that links obesity to skeletal muscle insulin resistance (2014)
  • Author(s): Fei Lan, H Misu, K Chikamoto, H Takayam, A Kikuchi, K Mohri, N Takata, H Hayashi, N Matsuzawa-Nagata, Y Takeshita, H Noda, Y Matsumoto, T Ota, T Nagano, M Nakagen, K Miyamoto, K Takatsuki, T Seo, K Iwayama, K Tokuyama, S Matsugo, H Tang, Y Saito, S Yamagoe, S Kaneko, T Takamura
  • Journal Title: Diabete Volume: 63 Issue: 5 Pages: 1649-1664
  • DOI: 10.2337/db13-0728
  • Peer Reviewed / Open Access
• [Journal Article] Increased serum leukocyte cell-derived chemotaxin 2 (LECT2) levels in obesity and fatty liver (2013)
  • Author(s): Akinori Okumura, Hiroyuki Unoki-Kubota, Yumi Matsushita, Tomoko Shiga, Yuriko Moriyoshi, Satoshi Yamagoe, Yasushi Kaburagi
  • Journal Title: BioScience Trends Volume: 7 Pages: 276-283
  • NAID: 130003397274
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of human leukocyte cell-derived chemotaxin 2 (LECT2) (2013)
  • Author(s): Zheng H, Miyakawa T, Sawano Y, Yamagoe S, Tanokura M
  • Journal Title: Acta Crystallogr Sect F Struct Biol Cryst Commun Volume: 69 Issue: 3 Pages: 316-319
  • DOI: 10.1107/s1744309113003758
  • Peer Reviewed
• [Journal Article] Leukocyte cell-derived chemotaxin 2 is a zinc-binding protein (2013)
  • Author(s): Okumura A, Suzuki T, Miyatake H, Okabe T, Hashimoto Y, Miyakawa T, Zheng H, Unoki-Kubota H, Ohno H, Dohmae N, Kaburagi Y, Miyazaki Y, Tanokura M, Yamagoe S
  • Journal Title: FEBS Lett Volume: 587 Issue: 5 Pages: 404-409
  • DOI: 10.1016/j.febslet.2013.01.025
  • Peer Reviewed
• [Journal Article] Expression, high-pressure refolding and purification of human leukocyte cell-derived chemotaxin 2 (LECT2). (2013)
  • Author(s): Hai Zheng, Takuya Miyakawa, Yoriko Sawano, Satoshi Yamagoe, Masaru Tanokura
  • Journal Title: Protein Expr. Purif. Volume: 88 Issue: 2 Pages: 221-229
  • DOI: 10.1016/j.pep.2013.01.008
  • Peer Reviewed
• [Journal Article] Increased serum leukocyte cell-derived chemotaxin 2 (LECT2) levels in obesity and fatty liver. (2013)
  • Author(s): Okumura A, Unoki-Kubota H, Matsushita Y, Shiga T, Moriyoshi Y, Yamagoe S, Kaburagi Y.
  • Journal Title: Biosci Trends. Volume: 7 Pages: 276-283
  • NAID: 130003397274
  • Peer Reviewed
• [Journal Article] Oncogenicβ-catenin triggers an inflammatory response that determines the aggressiveness of hepatocellular carcinoma in mice (2012)
  • Author(s): Marie Anson, Anne-Marie Crain-Denoyelle, Véronique Baud, Fanny Chereau, AngéliqueGougelet, Benoit Terris, Satoshi Yamagoe, Sabine Colnot, Mireille Viguier, Christine Perret, Jean-Pierre Couty
  • Journal Title: Journal Clinical Investigation Volume: 122 Issue: 2 Pages: 586-599
  • DOI: 10.1172/jci43937
  • Peer Reviewed
• [Presentation] Leukocyte cell-derived chemotaxin 2 is a zinc-binding protein : the monomeric forms are stabilized by the zinc ion (2013)
  • Author(s): Akinori Okumura, Takehiro Suzuki, Hideyuki Miyatake, Takuya Miyakawa, HaiZheng, Hiroyuki Unoki-Kubota, NaoshiDohmae, Yasushi Kaburagi, Masaru Tanokura, Satoshi Yamagoe
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] Hepatokine LECT2 causes insulin resistance in skeletal muscle throught increased JNK phosphorylation (2013)
  • Author(s): Fei Lan, Hirofumi Misu, Keita Chikamoto, Hiroaki Takayama, Akihiro Kikuchi, Satoshi Yamagoe, Shuichi Kaneko, Toshinari Takamura
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] Leukocyte cell-derived chemotaxin 2 is a zinc-binding protein: the monomeric forms are stabilized by the zinc ion. (2013)
  • Author(s): Akinori Okumura, Takehiro Suzuki, Hideyuki Miyatake, Takuya Miyagawa, Hai Zheng, Hiroyuki Unoki- Kubota, Naoshi Dohmae, Yasushi Kaburagi, Masaru Tanokura, Satoshi Yamagoe.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] Hepatokine LECT2 causes insulin resistance in skeletal muscle through increased JNK phosphorylation. (2013)
  • Author(s): Fei Lan, Hirohumi Misu, Keita Chikamoto, Hiroaki Takayam1, Akihiro Kikuchi, Satoshi Yamagoe, Shuichi Kaneko, Toshinari Takamura.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
• [Remarks] ホームページ
  • URL: http://www.geocities.jp/lect2_yamagoe/
• [Remarks] LECT2に関する研究
  • URL: http://www.geocities.jp/lect2_yamagoe/
• [Remarks] LECT2に関する研究
  • URL: http://www.geocities.jp/lect2_yamagoe/