Analysis of LECT2 receptor and its signal transduction
Project/Area Number |
23590275
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General physiology
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Research Institution | National Institute of Infectious Diseases |
Principal Investigator |
SATOSHI Yamagoe 国立感染症研究所, 真菌部, 主任研究官 (00212283)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 生理活性 / シグナル伝達 / LECT2 |
Research Abstract |
Accumulating evidences indicate that LECT2 is associated with several physiological phenomena, but its function is not fully understood. To make clear the function of LECT2, it is important to identify the LECT2 receptor. We performed expression cloning with a retroviral cDNA library constructed from a cell line that highly expressed the LECT2 receptor. Retrovirus infected cells binding LECT2-Fc fusion protein were enriched by cell sorting. The seven cell lines cloned from the enriched cells all had cDNAs derived from the same gene in integrated retrovirus vector. We checked the possibility that this protein was the LECT2 receptor by some experiments. Moreover, we analyzed the phosphoprotein involved in LECT2 signal transduction by western blotting with thirty different kinds of anti-phosphoprotein antibodies, identifying some possible phosphoproteins.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] LECT2 functions as a hepatokine that links obesity to skeletal muscle insulin resistance2014
Author(s)
Fei Lan, H Misu, K Chikamoto, H Takayam, A Kikuchi, K Mohri, N Takata, H Hayashi, N Matsuzawa-Nagata, Y Takeshita, H Noda, Y Matsumoto, T Ota, T Nagano, M Nakagen, K Miyamoto, K Takatsuki, T Seo, K Iwayama, K Tokuyama, S Matsugo, H Tang, Y Saito, S Yamagoe, S Kaneko, T Takamura
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Journal Title
Diabete
Volume: 63
Issue: 5
Pages: 1649-1664
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Leukocyte cell-derived chemotaxin 2 is a zinc-binding protein2013
Author(s)
Okumura A, Suzuki T, Miyatake H, Okabe T, Hashimoto Y, Miyakawa T, Zheng H, Unoki-Kubota H, Ohno H, Dohmae N, Kaburagi Y, Miyazaki Y, Tanokura M, Yamagoe S
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Journal Title
FEBS Lett
Volume: 587
Issue: 5
Pages: 404-409
DOI
Related Report
Peer Reviewed
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[Journal Article] Oncogenicβ-catenin triggers an inflammatory response that determines the aggressiveness of hepatocellular carcinoma in mice2012
Author(s)
Marie Anson, Anne-Marie Crain-Denoyelle, Véronique Baud, Fanny Chereau, AngéliqueGougelet, Benoit Terris, Satoshi Yamagoe, Sabine Colnot, Mireille Viguier, Christine Perret, Jean-Pierre Couty
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Journal Title
Journal Clinical Investigation
Volume: 122
Issue: 2
Pages: 586-599
DOI
Related Report
Peer Reviewed
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