| Project/Area Number |
23590284
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | University of Tokyo Health Sciences (2012-2013)
Nippon Medical School (2011) |
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Principal Investigator |
KATO Masakatsu 東京医療学院大学, 保健医療学部, 教授 (90143239)
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| Co-Investigator(Renkei-kenkyūsha) |
ISHII Hirotaka 日本医科大学, 医学部, 講師 (20445810)
SAKUMA Yasuo 東京医療学院大学, 保健医療学部, 学長・教授 (70094307)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | GnRH neuron / SK channel / Kv7 channel / Nalcn / ucn80 / HCN channel / Kv7 / KCNQ / Unc-80 / HCN / KCNQ channel / rat / GnRH-EGFP / transgenic / patch clamp / GnRHニューロン |
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| Research Abstract |
This study aimed to determine ion channels which closely related to the excitability of rat GnRH neurons. First, we analyzed K channels and found an involvement of somatostatin receptors (Sst), SK channels,and Kv7 channels. SK and Kv7 are involved in the generation of afterhyperpolarization. Second, cationic channels were analyzed in oligo-cell RT-PCR. GnRH neurons express Nalcn (Na leak channel), Unc-80 (associate protein to Nalc), and HCN (Hyperpolarization-activated cNucleotide gated Cationic channels).
These ion channels may determine the cell excitability of GnRH neurons.
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