| Project/Area Number |
23590311
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
General pharmacology
|
|---|
| Research Institution | Kanazawa Medical University |
|---|
Principal Investigator |
YOSHIDA Junko 金沢医科大学, 医学部, 講師 (20064628)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
IWABUCHI Kuniyoshi 金沢医科大学, 医学部, 教授 (10232696)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | ストア作動性カルシウム流入 / STIM1 / cell proliferation / cell migration / tumorigenicity / ヒト扁平上皮がん / 扁平上皮がん / migration / 細胞増殖 |
|---|
| Research Abstract |
Stromal interaction molecule 1 (STIM1) monitors the calcium levels in the endoplasmic reticulum and activates the plasma membrane calcium release-activated calcium (CRAC) channels to induce the store operated calcium entry (SOCE). To explore the posible roles of STIM1 in human epidermoid carcinoma A431 cell growth, we established the STIM1 knockdown A431 clones by RNA interference. The SOCE, cell proliferation, cell migration and the xenograft growth in nude mice were reduced in the knockdown clones compared to a negative control clone. Re-expression of a siRNA-resistant full-length STIM1, but not CRAC activation domain (CAD)-deleted STIM1 mutant, in the knockdown clones restored the amplitude of SOCE and cell migration. These results indicate that STIM1 plays an important role in SOCE, cell growth and tumorigenicity in human epidermoid A431 cells, suggesting the potential use of STIM1-targeting agents for treating epidermoid carcinoma.
|
