| Project/Area Number |
23590319
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KITA Satomi 福岡大学, 医学部, 講師 (10461500)
ARAI Yuji 独立行政法人国立循環器病センター, バイオサイエンス部, 部長 (30202724)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | イオン輸送体 / Ca2+シグナル / 腎上皮細胞 / 腎機能 / イオン輸送 / 酵母two-hybrid / 蛋白質相互作用 |
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| Research Abstract |
Na+/Ca2+ exchanger type 1 (NCX1) bidirectionally exchanges 3 Na+ with 1 Ca2+, depending on the electrochemical gradients across the plasma membrane. In renal tubule, NCX1 is localized to the basolateral membranes of distal convoluted tubule cells, and is considered to be involved in Ca2+ reabsorption. However, the molecular mechanisms for its membrane sorting remain to be elucidated. In the present study, we found that the intracellular domain of NCX1 interacts with the adaptor protein. Using GST pull-down assays, we further identified the binding motif in NCX1 molecule. We also found that the mutations in the binding motif of NCX1 affect its membrane sorting in epithelial MDCK cells, suggesting the interaction between NCX1 and the adaptor protein plays a key role in NCX1 basolateral sorting.
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