2013 Fiscal Year Final Research Report
Molecular mechanism for targeting of NCX1 to basolateral membrane in renal epithelial cells
| Project/Area Number |
23590319
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KITA Satomi 福岡大学, 医学部, 講師 (10461500)
ARAI Yuji 独立行政法人国立循環器病センター, バイオサイエンス部, 部長 (30202724)
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| Project Period (FY) |
2011 – 2013
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| Keywords | イオン輸送体 / Ca2+シグナル / 腎上皮細胞 / 腎機能 |
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| Research Abstract |
Na+/Ca2+ exchanger type 1 (NCX1) bidirectionally exchanges 3 Na+ with 1 Ca2+, depending on the electrochemical gradients across the plasma membrane. In renal tubule, NCX1 is localized to the basolateral membranes of distal convoluted tubule cells, and is considered to be involved in Ca2+ reabsorption. However, the molecular mechanisms for its membrane sorting remain to be elucidated. In the present study, we found that the intracellular domain of NCX1 interacts with the adaptor protein. Using GST pull-down assays, we further identified the binding motif in NCX1 molecule. We also found that the mutations in the binding motif of NCX1 affect its membrane sorting in epithelial MDCK cells, suggesting the interaction between NCX1 and the adaptor protein plays a key role in NCX1 basolateral sorting.
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[Journal Article] Dietary salt intake regulates WNK3-SPAK-NKCC1 phosphorylation cascade in mouse aorta through angiotensin II2013
Author(s)
Zeniya M, Sohara E, Kita S, Iwamoto T, Susa K, Mori T, Oi K, Chiga M, Yang SS, Lin SH, Rai T, Sasaki S, Uchida S
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Journal Title
Hypertension
Volume: 62(5)
Pages: 872-878
DOI
Peer Reviewed
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[Journal Article] Pretreatment of donor islets with the Na^+/Ca^<2+> exchanger inhibitor improves the efficiency of islet transplantation2013
Author(s)
Mera T†, Itoh T†, Kita S†, (†co-first authors) Kodama S, Kojima D, Nishinakamura H, Okamoto K, Ohkura M, Nakai J, Iyoda T, Iwamoto T, Matsuda T, Baba A, Omori K, Ono J, Watarai H, Taniguchi M, Yasunami Y
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Journal Title
Am J Transplant
Volume: 13(8)
Pages: 2154-2160
DOI
Peer Reviewed
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[Journal Article] Structural basis for the counter-transport mechanism of a H^+/Ca^<2+> exchanger2013
Author(s)
Nishizawa T, Kita S, Maturana AD, Furuya N, Hirata K, Kasuya G, Ogasawara S, Dohmae N, Iwamoto T, Ishitani R, Nureki O
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Journal Title
Science
Volume: 341(6142)
Pages: 168-172
DOI
Peer Reviewed
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[Journal Article] Preferential involvement of Na⁺/Ca²⁺ exchanger type-1 in the brain damage caused by transient focal cerebral ischemia in mice2012
Author(s)
Morimoto N, Kita S, Shimazawa M, Namimatsu H, Tsuruma K, Hayakawa K, Mishima K, Egashira N, Iyoda T, Horie I, Gotoh Y, Iwasaki K, Fujiwara M, Matsuda T, Baba A, Komuro I, Horie K, Takeda J, Iwamoto T, Hara H.
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Journal Title
Biochem Biophys Res Commun
Volume: 429(3-4)
Pages: 186-190
DOI
Peer Reviewed
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[Journal Article] Effect of heterozygous deletion of WNK1 on the WNK-OSR1/SPAK-NCC/NKCC1/NKCC2 signal cascade in the kidney and blood vessels2012
Author(s)
Susa K, Kita S, Iwamoto T, Yang SS, Lin SH, Ohta A, Sohara E, Rai T, Sasaki S, Alessi DR, Uchida S
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Journal Title
Clin Exp Nephrol
Volume: 16(4)
Pages: 530-538
Peer Reviewed
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[Journal Article] TRIC-A channels in vascular smooth muscle contribute to blood pressure maintenance2011
Author(s)
Yamazaki D, Tabara Y, Kita S, Hanada H, Komazaki S, Naitou D, Mishima A, Nishi M, Yamamura H, Yamamoto S, Kakizawa S, Miyachi H, Yamamoto S, Miyata T, Kawano Y, Kamide K, Ogihara T, Hata A, Umemura S, Soma M, Takahashi N, Imaizumi Y, Miki T, Iwamoto T, Takeshima H
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Journal Title
Cell Metab
Volume: 14(2)
Pages: 231-241
DOI
Peer Reviewed
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[Journal Article] Cilostazol suppresses angiotensin II-induced vasoconstriction via protein kinase A-mediated phosphorylation of the transient receptor potential canonical 6 channel2011
Author(s)
Nishioka K, Nishida M, Ariyoshi M, Jian Z, Saiki S, Hirano M, Nakaya M, Sato Y, Kita S, Iwamoto T, Hirano K, Inoue R, Kurose H
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Journal Title
Arterioscler Thromb Vasc Biol
Volume: 31(10)
Pages: 2278-2286
DOI
Peer Reviewed
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