Investigation of the function of non-coding RNA in the morphogenesis

• Project/Area Number: 23590326
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: KURIHARA YUKIKO 東京大学, 医学(系)研究科(研究院), 講師 (80345040)
• Co-Investigator(Renkei-kenkyūsha): KURIHARA Hiroki 東京大学, 大学院医学研究科, 教授 (20221947) ; UCHIJIMA Yasunobu 東京大学, 大学院医学研究科, 助手 (90272426)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: エンドセリン / 下顎形成 / 非コードRNA / 発生医学 / 転写調節 / 形態形成 / 遺伝子発現制御 / 発生

Research Abstract

Endothelin-1/Ednra signal determines mandibular identity. Ednra homozygotes exhibited homeotic transformation of the lower jaw into an upper jaw. When we expressed the Evf2 of long noncoding in the Ednra homozygous by RMCE (recombination mediated cassette exchange), the distal part of mandible was aggravated. Evf2 controls the expressions of principal genes under Endothelin-1/Ednra signaling directly and indirectly. DNA methylation and long non-coding RNA were possibly suggested as the underlying mechanism.

Research Products

• [Journal Article] Calpain-6 deficiency promotes skeletal muscle development and regeneration (2013)
  • Author(s): Tonami K, Hata S, Ojima K, Ono Y, Kurihara Y, Amano T, Sato T, Kawamura Y, Kurihara H, Sorimachi H
  • Journal Title: PLoS Genet Volume: 9(8)
• [Journal Article] Endothelin regulates neural crest deployment and fate to form great vessels through Dlx5/Dlx6-independent mechanisms (2013)
  • Author(s): Kim KS, Arima Y, Kitazawa T, Nishiyama K, Asai R, Uchijima Y, Sato T, Levi G, Kitanaka S, Igarashi T, Kurihara Y, Kurihara H
  • Journal Title: Mech Dev Volume: 130(11-12) Pages: 553-566
• [Journal Article] Endothelin regulates neural crest deployment and fate to form great vessels through D1x5/D1x6-independent mechanisms. (2013)
  • Author(s): Kim KS, Arima Y, Kitazawa T, Nishiyama K, Asai R, Uchijima Y, Sato T, Levi G, Kitanaka S, Igarashi T, Kurihara Y, Kurihara H.
  • Journal Title: Mechanism of Development Volume: 130 Issue: 11-12 Pages: 553-566
  • DOI: 10.1016/j.mod.2013.07.005
  • Peer Reviewed / Open Access
• [Journal Article] Calpain-6 deficiency promotes skeletal muscle development and regeneration. (2013)
  • Author(s): Tonami, K., Hata, S., Ojima, K., Ono, Y., Kurihara, Y., Amano, T., Sato, T., Kawamura, Y., Kurihara, H., Sorimachi, H.
  • Journal Title: PLOS Genetics Volume: 9 Issue: 8 Pages: 1003668-1003668
  • DOI: 10.1371/journal.pgen.1003668
  • Peer Reviewed / Open Access
• [Journal Article] Preotic neural crest cells contribute to coronary artery smooth muscle involving endothelin signalling (2012)
  • Author(s): Arima Y, Miyagawa-Tomita S, Maeda K, Asai R, Seya D, Minoux M, Rijli FM, Nishiyama K, Kim KS, Uchijima Y, Ogawa H, Kurihara Y, Kurihara H
  • Journal Title: Nat Commun Volume: 3 Pages: 1267-1267
• [Journal Article] Preotic neural crest cells contribute to coronary smooth muscle involving endothelin signalling. (2012)
  • Author(s): Yuichiro Arima
  • Journal Title: Nature Communications Volume: 3 Issue: 1 Pages: 1267-1267
  • DOI: 10.1038/ncomms2258
  • Peer Reviewed
• [Journal Article] Angiogenic morphogenesis driven by dynamic and heterogeneous collective endothelial cell movement (2011)
  • Author(s): Arima S, Nishiyama K, Ko T, Arima Y, Hakozaki Y, Sugihara K, Koseki H, Uchijima Y, Kurihara Y, Kurihara H
  • Journal Title: Development Volume: 138(21) Pages: 4763-4776
• [Journal Article] Identification and developmental analysis of endothelin receptor type-A expressing cells in the mouse kidney (2011)
  • Author(s): Kitazawa T, Sato T, Nishiyama K, Asai R, Arima Y, Uchijima Y, Kurihara Y, Kurihara H
  • Journal Title: Gene Expr Patterns Volume: 11(7) Pages: 371-377
• [Journal Article] Calpain-6, a microtubule-stabilizing protein, regulates Rac1 activity and cell motility through interaction with GEF-H1 (2011)
  • Author(s): Tonami K, Kurihara Y, Arima S, Nishiyama K, Uchijima Y, Asano T, Sorimachi H, Kurihara H
  • Journal Title: J Cell Sci Volume: 124(Pt 8) Pages: 1214-1223
• [Journal Article] Calcitonin gene-related peptide facilitates revascularization during hindlimb ischemia in mice (2011)
  • Author(s): Mishima T, Ito Y, Hosono K, Tamura Y, Uchida Y, Hirata M, Suzsuki T, Amano H, Kato S, Kurihara Y, Kurihara H, Hayashi I, Watanabe M, Majima M
  • Journal Title: Am J Physiol Heart Circ Physiol Volume: 300(2)
• [Journal Article] Angiogenic morphogenesis driven by dynamic and heterogeneous collective endothelial movement (2011)
  • Author(s): Satoshi Arima
  • Journal Title: Development Volume: 138 Pages: 4763-4776
  • Peer Reviewed
• [Journal Article] Identification and developmental analysis of endothelin receptor type-A expressing cells in the mouse kidney (2011)
  • Author(s): Kitazawa T, Sato T, Nishiyama K, Asai R, Arima Y, Uchijima Y, Kurihara Y, Kurihara H.
  • Journal Title: Gene Expr. Patterns Volume: 11 Issue: 7 Pages: 371-377
  • DOI: 10.1016/j.gep.2011.04.001
  • Peer Reviewed
• [Presentation] Hoxa2 in neural crest cells re-patterns craniofacial structures: (2013)
  • Author(s): Taro Kitazawa
  • Organizer: 第46回日本発生生物学会
  • Place of Presentation: くにびきメッセ（松江）
• [Presentation] Development of the tympanic membrane and endothelin-Dlx5/6 pathway: Comparative embryology between mammals and reptiles (2013)
  • Author(s): Taro Kitazawa
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（神戸）
• [Presentation] 下顎形成におけるエンドセリンシグナルにおけるnon-coding RNAとパラスペックル蛋白の作用 (2012)
  • Author(s): 栗原由紀子,内島泰信,櫛山櫻,濱崎真夏,西山功一,栗原裕基
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場(福岡県福岡市)
  • Year and Date: 2012-12-12
• [Presentation] Involvement of non-coding RNA Evf2 in the endothelin signaling in branchial arch development (2012)
  • Author(s): Yukiko Kurihara, Yasunobu Uchijima, Sakura Kushiyama, Rieko Asai, Hiroki Kurihara
  • Organizer: Cell Symposia"Functional RNA"
  • Place of Presentation: Sitges (Spain)
  • Year and Date: 2012-12-03
• [Presentation] Involvement of non-coding RNA : Evf2 in the Endothelin signaling in branchial arch development (2012)
  • Author(s): Yukiko Kurihara, Yasunobu Uchijima, Sakura Kushiyama, Hiroki Kurihara
  • Organizer: Keystone Symposia Canference"Non-Coding RNAs"
  • Place of Presentation: Snowbird Cliff lodge (USA)
  • Year and Date: 2012-04-02
• [Presentation] Involvement of non-coding RNA : Evf2 in the Endothelin signaling in branchial arch development (2012)
  • Author(s): Yukiko Kurihara
  • Organizer: 第35回 日本分子細胞生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡(福岡県）
• [Presentation] Involvement of non-coding RNA Evf2 in the endothelin signaling in branchial arch development (2012)
  • Author(s): Yukiko Kurihara
  • Organizer: Cell Symposia; Functional RNAs
  • Place of Presentation: Hotel Melia, Sitges, Spain
• [Presentation] MicroRNA 199/214 cluster is required for normal growth and skeletal development in mice (2012)
  • Author(s): Yumiko Kawamura
  • Organizer: Cell Symposia; Functional RNAs
  • Place of Presentation: Hotel Melia, Sitges, Spain
• [Presentation] Involvement of non-coding RNA: Evf2 in the Endothelin signaling in branchial arch development (2012)
  • Author(s): Yukiko Kurihara
  • Organizer: Keystone Symposia Canference : Non-Coding RNAs
  • Place of Presentation: スノウ･バード・クリフ・ロッジ（米国・ユタ州）
• [Presentation] Involvement of non-coding RNA : Evf2 in the Endothelin signaling in branchial arch development (2011)
  • Author(s): Yukiko Kurihara, Yasunobu Uchijima, Sakura Kushiyama, Taro Kitazawa, Rieko Asai, Hiroki Kurihara
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
  • Year and Date: 2011-12-13
• [Presentation] 下顎形成におけるエンドセリンシグナルにおけるnon-coding RNAとパラスペックル蛋白の作用 (2011)
  • Author(s): 栗原由紀子
  • Organizer: 第34回 日本分子生物学会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
• [Presentation] Endothelin receptor type A expressing cell population in the inflow tract contributes to chamber formation
  • Author(s): Rieko Asai
  • Organizer: Weinstein Cardiovascular Conference 2013
  • Place of Presentation: USA, Tucson, Arizona
• [Presentation] Endothelin receptor type A expressing cell population in the inflow tract contributes to chamber formation
  • Author(s): Rieko Asai
  • Organizer: The 7th TAKAO International Symposium
  • Place of Presentation: 代々木（東京）
• [Presentation] 病態生理におけるSirt3とp53シグナルの関連
  • Author(s): 河村悠美子
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（神戸）
• [Remarks] 東京大学大学院医学系研究科 代謝生理化学教室
  • URL: http://bio.m.u-tokyo.ac.jp/index.html
• [Remarks] 東京大学 大学院 医学系研究科 代謝生理化学教室
  • URL: http://bio.m.u-tokyo.ac.jp/home-j.html
• [Remarks]
  • URL: http://bio.m.u-tokyo.ac.jp/home-j.html