Function of novel BRCA2-associating proteins in centrosomal regulation and DNA repair
| Project/Area Number |
23590355
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
TAKENAKA Katsuya 東京医科歯科大学, 難治疾患研究所, 助教 (20378706)
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| Co-Investigator(Kenkyū-buntansha) |
OGI Tomoo 長崎大学, 7原爆後障害医療研究所, 准教授 (80508317)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 乳癌 / BRCA / 中心体 / 画像認識 / BRCA2 / DNA損傷修復 / 中心体複製 / タンパク質間相互作用 / DR-GFP / U2OS |
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| Research Abstract |
BRCA2 germline mutations account for the majority of heredity breast and ovarian cancer. A major function of BRCA2 is known as a regulator of homologous recombination in DNA damage repair. In addition, BRCA2 also plays an important role in centrosomal regulation, whose dysfunction might be involved in
the tumorigenesis of breast cancer. Even so, detail molecular mechanism is
not yet uncovered. In the present study, we tried to locate a molecular region of BRCA2 responsible for this function. Series of BRCA2 fragments were exogenously expressed in human cultured cells. Numbers of centrosome in a cell are counted to see if an overexpression of a specific fragment could impair a numerical integrity of centrosomes. For this purpose we developed an automated centrosome-counting system based on image recognition, which allows us to judge a large number of transfected cells without human bias.
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Report
(4 results)
Research Products
(6 results)
