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The molecular basis of morphological atypism (proteome analysis of KRAS downstream factors)

Research Project

Project/Area Number 23590428
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Human pathology
Research InstitutionYokohama City University

Principal Investigator

OKUDELA KOJI  横浜市立大学, 医学部, 講師 (10326027)

Co-Investigator(Kenkyū-buntansha) KATAYA Akira  日本医科大学, 医学部, 助教 (10333113)
NAGAHARA Noriyuki  日本医科大学, 医学部, 准教授 (10208043)
WOO Tetsukan  横浜市立大学, 医学研究科, 共同研究員 (90537177)
YAZAWA Takuya  杏林大学, 医学部, 准教授 (50251054)
SHIMOYAMADA Hiroaki  杏林大学, 医学部, 助教 (60381472)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsatypism / lung cancer / 肺癌 / 異型性 / CLIC4
Research Abstract

A purpose of present study is to elucidate a molecular basis of morphological atypism. This study investigated the proteome modulated by oncogenic KRAS in immortalized airway epithelial cells. We here focused on CLIC4 to investigate its potential involvement in an induction of the morphologic atypism in lung cancers. CILC4 protein levels were reduced in some lung cancer cell lines. The knockdown of CLIC4 in an immortalized airway cell line modulated a cell shape as marked enlargement of cells and nuclei. CLIC4 protein levels tended to be lower in high-grade tumors. These results suggest that the alteration in CLIC4 could induce some morphological atypism of neoplastic cells and could be involved in carcinogenesis of the lung.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (34 results)

All 2014 2013 2012 2011 Other

All Journal Article (26 results) (of which Peer Reviewed: 26 results,  Open Access: 2 results) Presentation (6 results) (of which Invited: 1 results) Book (2 results)

  • [Journal Article] Two cases of pulmonary sarcoidosis presenting with subpleural reticular shadows in the lower lobes2014

    • Author(s)
      Sekine A, Takemura T, Baba T, Oda T, Iwasawa T, Okudela K, Ogura T
    • Journal Title

      Intern Med

      Volume: 53 Pages: 455-460

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of successively transplantable rabbit VX2 cancer cells that express enhanced green fluorescent protein2014

    • Author(s)
      Oshiro H, Fukumura H, Nagahama K, Sato I, Sugiura K, Iobe H, Okiyama E, Nagao T, Nagashima Y, Aoki I, Yamanaka S, Murakami A, Maegawa J, Chishima T, Ichikawa Y, Ishikawa Y, Nagai T, Nomura M, Ohashi K, Okudela K
    • Journal Title

      Med Mol Morphol

      Volume: 47 Issue: 3 Pages: 130-136

    • DOI

      10.1007/s00795-013-0052-x

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proteome Analysis for Downstream Targets of Oncogenic KRAS - the Potential Participation of CLIC4 in Carcinogenesis in the Lung2014

    • Author(s)
      Okudela K, Katayama A, Woo T, Mitsui H, Suzuki T, Tateishi Y, Umeda S, Tajiri M, Masuda M, Nagahara N, Kitamura H, Ohashi K
    • Journal Title

      PLoS One

      Volume: 9

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Diffuse alveolar hemorrhage as a fatal adverse effect of bevacizumab : an autopsy case2014

    • Author(s)
      Ikeda S, Sekine A, Kato T, Yoshida M, Ogata R, Baba T, Nagahama K, Okudela K, Ogura T
    • Journal Title

      Jpn J Clin Oncol

      Volume: 44 Pages: 497-500

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lung abscess combined with chronic osteomyelitis of the mandible successfully treated with video-assisted thoracoscopic surgery2014

    • Author(s)
      Arai H, Inui K, Watanabe K, Watanuki K, Okudela K, Tsuboi M, Masuda M
    • Journal Title

      Clin Respir J

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of successively transplantable rabbit VX2 cancer cells that express enhanced green fluorescent protein2014

    • Author(s)
      Oshiro H, Fukumura H, Nagahama K, Sato I, Sugiura K, Iobe H, Okiyama E, Nagao T, Nagashima Y, Aoki I, Yamanaka S, Murakami A, Maegawa J, Chishima T, Ichikawa Y, Ishikawa Y, Nagai T, Nomura M, Ohashi K and Okudela K
    • Journal Title

      Med Mol Morphol

      Volume: 印刷中 Issue: 1 Pages: 13-23

    • DOI

      10.1007/s00795-014-0071-2

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expression of HDAC9 in lung cancer-potential role in lung carcinogenesis. Int J Clin Exp Pathol 7: 213-220.2014

    • Author(s)
      Okudela K, Mitsui H, Suzuki T, Woo T, Tateishi Y, Umeda S, Saito Y, Tajiri M, Masuda M and Ohashi K
    • Journal Title

      Int J Clin Exp Pathol

      Volume: 7 Pages: 213-220

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diffuse alveolar hemorrhage as a fatal adverse effect of bevacizumab2014

    • Author(s)
      Ikeda S, Sekine A, Kato T, Yoshida M, Ogata R, Baba T, Nagahama K, Okudela K and Ogura T
    • Journal Title

      Jpn J Clin Oncol

      Volume: 44 Issue: 5 Pages: 497-500

    • DOI

      10.1093/jjco/hyu023

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteome analysis for downstream targets of oncogenic KRAS--the potential participation of CLIC4 in carcinogenesis in the lung.2014

    • Author(s)
      Okudela K, Katayama A, Woo T, Mitsui H, Suzuki T, Tateishi Y, Umeda S, Tajiri M, Masuda M, Nagahara N, Kitamura H and Ohashi K
    • Journal Title

      PLoS One

      Volume: 9 Issue: 2 Pages: 1-10

    • DOI

      10.1371/journal.pone.0087193

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lung abscess combined with chronic osteomyelitis of the mandible successfully treated with video-assisted thoracoscopic surgery2014

    • Author(s)
      Arai H, Inui K, Watanabe K, Watanuki K, Okudela K, Tsuboi M and Masuda M
    • Journal Title

      Clin Respir J

      Volume: 印刷中 Issue: 2 Pages: 253-256

    • DOI

      10.1111/crj.12115

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hepatocyte nuclear factor-1 alpha inactivated hepatocellular adenomas in patient with congenital absence of the portal vein : a case report2013

    • Author(s)
      Tateishi Y, Furuya M, Kondo F, Torii I, Nojiri K, Tanaka Y, Umeda S, Okudela K, Inayama Y, Endo I, Ohashi K
    • Journal Title

      Pathol Int

      Volume: 63 Pages: 358-363

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Downregulation of ALDH1A1 expression in non-small cell lung carcinomas--its clinicopathologic and biological significance2013

    • Author(s)
      Okudela K, Woo T, Mitsui H, Suzuki T, Tajiri M, Sakuma Y, Miyagi Y, Tateishi Y, Umeda S, Masuda M, Ohashi K
    • Journal Title

      Int J Clin Exp Pathol

      Volume: 6 Pages: 1-12

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of HDAC9 in lung cancer - potential role in lung carcinogenesis2013

    • Author(s)
      Okudela K, Mitsui H, Suzuki T, Woo T, Tateishi Y, Umeda S, Saito Y, Tajiri M, Masuda M, Ohashi K
    • Journal Title

      Int J Clin Exp Pathol

      Volume: 7 Pages: 213-220

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] An association between nuclear morphology and immunohistochemical expression of p53 and p16INK4A in lung cancer cells2013

    • Author(s)
      Okudela K
    • Journal Title

      Med Mol Morphol

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Unique mutation, accelerated mTOR signaling and angiogenesis in the pulmonary cysts of Birt-Hogg-Dube syndrome2013

    • Author(s)
      Nishii T, Tanabe M, Tanaka R, Matsuzawa T, Okudela K, Nozawa A, Nakatani Y and Furuya M
    • Journal Title

      Pathol Int

      Volume: 63 Pages: 45-55

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Elevated microsatellite alterations at selected tetra-nucleotide (EMAST) in non-small cell lung cancers--a potential determinant of susceptibility to multiple malignancies2013

    • Author(s)
      Arai H, Okudela K, Oshiro H, Komitsu N, Mitsui H, Nishii T, Tsuboi M, Nozawa A, Noishiki Y, Ohashi K, Inui K, Masuda M
    • Journal Title

      Int J Clin Exp Pathol

      Volume: 6 Pages: 395-410

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hepatocyte Nuclear Factor-1ΑInactivated Hepato- cellular Adenomas in Patient With Congenital Absence of The Portal Vein2013

    • Author(s)
      Tateishi Y, Furuya M, Kondo F, Okudela K, Umeda S, Ohashi K
    • Journal Title

      Pathol Int

      Volume: vol 63 Issue: 7 Pages: 358-363

    • DOI

      10.1111/pin.12072

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Downregulation of ALDH1A1 expression in non-small cell lung carcinomas - its clinicopathologic and biological significance2013

    • Author(s)
      Okudela K, Woo T, Mitsui H, Suzuki T, Tajiri M, et al.
    • Journal Title

      Int J Clin Exp Pathol

      Volume: 6 Pages: 1-12

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Prognostic value of the IASLC/ATS/ERS classification of lung adenocarcinoma in stage I disease of Japanese cases2013

    • Author(s)
      Woo T, Okudela K, Mitsui H, Tajiri M, Yamamoto T, et al.
    • Journal Title

      Pathol Int

      Volume: 62 Issue: 12 Pages: 785-791

    • DOI

      10.1111/pin.12016

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Prognostic value of the IASLC/ATS/ERS classification of lung adenocarcinoma in stage I disease of Japanese cases2012

    • Author(s)
      Woo T, Okudela K, Mitsui H, Tajiri M, Yamamoto T, Rino Y, Ohashi K, Masuda M
    • Journal Title

      Pathol Int

      Volume: 62 Pages: 785-791

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of the potential cancer stem cell markers, CD133, CD44, ALDH1, and beta-catenin, in primary lung adenocarcinoma--their prognostic significance2012

    • Author(s)
      Okudela K, Woo T, Mitsui H, Tajiri M, Masuda M, Ohashi K
    • Journal Title

      Pathol Int

      Volume: 62 Pages: 792-801

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of miR-30d as a novel prognostic maker of prostate2012

    • Author(s)
      Kobayashi N, Uemura H, Nagahama K, Okudela K, Furuya M, Ino Y, Ito Y, Hirano H, Inayama Y, Aoki I, Nagashima Y, Kubota Y, Ishiguro H
    • Journal Title

      Oncotarget

      Volume: 3 Pages: 1455-71

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pulmonary adenocarcinoma, Lambert--Eaton myasthenic syndrome, and voltage-gated calcium channels : a case report2012

    • Author(s)
      Arai H, Inui K, Hashimoto K, Kan OK, Nishii T, Kishida H, Okudela K, Nozawa A, Kaneko T, Masuda M
    • Journal Title

      J Med Case Rep

      Volume: 6 Pages: 281-281

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of the potential cancer stem cell markers, CD133, CD44, ALDH1, and beta-catenin, in primary lung adenocarcinoma-their prognostic significance2012

    • Author(s)
      Okudela K, Woo T, Mitsui H, Tajiri M, Masuda M, et al.
    • Journal Title

      Pathol Int

      Volume: 62 Issue: 12 Pages: 792-801

    • DOI

      10.1111/pin.12019

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Severe obliterative bronchitis associated with Stevens-Johnson syndrome2011

    • Author(s)
      Woo T, Saito H, Yamakawa Y, Komatsu S, Onuma S, Okudela K, Nozawa A, Aihara M, Ikezawa Z, Ishigatsubo Y
    • Journal Title

      Intern Med

      Volume: 50 Pages: 2823-2827

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The morphologic atypism of epithelial neoplasms; its molecular basis and pathologic significance2011

    • Author(s)
      Okudela-K
    • Journal Title

      横浜医学

      Volume: 62 Pages: 51-68

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Significant association between EGFR-mutated lung adenocarcinoma and past illness from gastric cancer or uterine myoma2014

    • Author(s)
      奥寺康司, 他
    • Organizer
      第103回日本病理学会総会
    • Place of Presentation
      広島
    • Year and Date
      2014-04-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Proteome analysis for downstream targets of KRAS - CLIC4 in lung carcinogenesis -2014

    • Author(s)
      奥寺康司、立石陽子、梅田茂明、三井秀昭、鈴木健久、禹哲漢、田尻道彦、大橋健一
    • Organizer
      第103回 日本病理学会総会
    • Place of Presentation
      広島国際会議場(広島)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 肺癌の発生・進展におけるKRAS-miR-31経路の意義2013

    • Author(s)
      奥寺康司、立石陽子、梅田茂明、三井秀昭、鈴木健久、禹哲漢、田尻道彦、大橋健一
    • Organizer
      第102回 日本病理学会総会
    • Place of Presentation
      札幌文芸館(札幌)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Proteome Analysis for Downstream Targets of Oncogenic KRAS - the Potential Participation of CLIC4 in Carcinogenesis in the Lung2012

    • Author(s)
      奥寺康司, 他
    • Organizer
      第101回日本病理学会総会
    • Place of Presentation
      東京
    • Year and Date
      2012-04-26
    • Related Report
      2013 Final Research Report
  • [Presentation] 気管支の上皮筋肉上皮腫の一例2011

    • Author(s)
      奥寺康司、矢澤卓也、田尻道彦、下山田博明、北村均
    • Organizer
      日本病理学会総会(第100回)
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Significant association between EGFR-mutated lung adenocarcinoma and past illness from gastric cancer or uterine myoma

    • Author(s)
      奥寺康司他
    • Organizer
      第101回 日本病理学会総会
    • Place of Presentation
      京王プラザホテル(東京)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Book] 呼吸と循環 「肺癌個別化治療におけるバイオマーカー」2012

    • Author(s)
      奥寺 康司、瀧川 奈義夫、岡本 邦男、間野 博行、清水 哲男
    • Total Pages
      5
    • Publisher
      医学書院
    • Related Report
      2012 Research-status Report
  • [Book] Cancer Stem Cells Theories and Practice2011

    • Author(s)
      Koji Okudela, Noriyuki Nagahara, Akira Katayama, Hitoshi Kitamura
    • Publisher
      InTech. Publication
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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