| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
We identified side population (SP fraction), which is thought to represent cancer stem cell like population and non-SP fraction, which is thought to represent differentiated cells in Hodgkin lymphoma cell lines, KMH2 and L428. SP consists of small cells, whereas non-SP contains relatively large mono nucleated cells and Hodgkin, Reed-Sternberg (H-RS) cells. CD30, a marker for HL is strongly expressed on both SP and non-SP. Both fractions also revealed strong activation of NF-kB. We sorted SP and non-SP and examined expression of mRNA using microarray analyses. The results indicated that molecules related to anti-oxidative stress are more frequently expressed in SP than non-SP. We also found that multi-nucleated cells are generated by abortive mitosis.
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