| Project/Area Number |
23590436
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
HONDA Kazuho 東京女子医科大学, 医学部, 准教授 (10256505)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 癌 / 腹膜播種 / 腹水 / 腹膜透析 / 中皮 / マクロファージ / フローサイトメトリー / 癌性腹膜炎 / 腹膜硬化症 / 007 / 008 / 029 / 007 細胞組織 / 008 生体分子 / 029 癌 |
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| Outline of Final Research Achievements |
The phenotypic alterations of mesothelial cells and macrophages in cancer ascites and peritoneal dialysis fluid (PDF) were investigated by flowcytometry. In cancer ascites, the expressions of podoplanin and CD44 in mesothelial cells tended to be increased but not significant, whereas the expressions of podoplanin, CD44 and CD14 were increased significantly. In PDF, the expression of CD44 in mesothelial cells was increased in some of long-term PD patients. The CD44 expression in macrophages was positively correlated with PD duration. CD44 is a hyaluronic acid receptor and works with podoplanin. They interact with ERMs and Rho GTPases affecting intracellular actin rearrangement. These interactions are associated with cell motility and migration in various processes of development, inflammation and cancer. These results indicated that the expression of surface molecules associated with cellular responses increased in mesothelial cells and macrophages in various peritoneal disorders.
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