Role of miRNA on tumor-stromal interaction in the pancreas

• Project/Area Number: 23590469
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Nagoya City University
• Principal Investigator: FUKAMACHI Katsumi 名古屋市立大学, 医学(系)研究科(研究院), 講師 (90381798)
• Co-Investigator(Renkei-kenkyūsha): TSUDA Hiroyuki 名古屋市立大学, その他部局, 教授 (10163809)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 膵癌 / 膵がん

Outline of Final Research Achievements

Pancreatic ductal adenocarcinoma is an extreamly stroma-rich and highly aggressive solid tumor. We established an animal model for human pancreatic ductal adenocarcinoma using transgenic rats in which expression of a human KRAS oncogene is regulated by the Cre/loxP system. Using this model, we searched for candidate extracellular miRNAs for participating in the stroma formation in pancreatic cancer. Many miRNAs showed different expression patterns between tumor tissue and serum of rats bearing pancreatic ductal adenocrcinoma. It is suggest that the some kind of sorted miRNAs were secreted to extracellular. These miRNA might be involved in the stroma formation.

Research Products

• [Journal Article] Rat N-ERC/mesothelin as a marker for in vivo screening of drugs against pancreas cancer (2014)
  • Author(s): Fukamachi K, Iigo M, Hagiwara Y, Shibata K, Futakuchi M, Alexander DB, Hino O, Suzui M, Tsuda H.
  • Journal Title: PLoS One Volume: 9 Issue: 10 Pages: 268-274
  • DOI: 10.1371/journal.pone.0111481
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A novel reporter rat strain that expresses LacZ upon Cre-mediated recombination (2013)
  • Author(s): Fukamachi K., Tanaka H., Sakai Y., Alexander D.B., Futakuchi M., Tsuda H., Suzui M.
  • Journal Title: Genesis Volume: 51 Issue: 4 Pages: 268-274
  • DOI: 10.1002/dvg.22371
  • Peer Reviewed
• [Journal Article] Twenty-one proteins up-regulated in human H-ras oncogene transgenic rat pancreas cancers are up-regulated in human pancreas cancer (2013)
  • Author(s): Yabushita S., Fukamachi K., Kikuchi F., Ozaki M., Miyata K., Sukata T., Deguchi Y., Tanaka H., Kakehashi A., Kawamura S., Uwagawa S., Wanibuchi H., Suzui M., Alexander D.B., Tsuda H.
  • Journal Title: Pancreas Volume: 42 Issue: 6 Pages: 1034-1039
  • DOI: 10.1097/mpa.0b013e3182883624
  • Peer Reviewed
• [Journal Article] Metabolomic and transcriptomic profiling of human K-ras oncogene transgenic rats with pancreatic ductal adenocarcinomas (2013)
  • Author(s): Yabushita S., Fukamachi K., Tanaka H., Fukuda T., Sumida K., Deguchi Y., Mikata K., Nishioka K., Kawamura S., Uwagawa S., Suzui M., Alexander D.B., Tsuda H.
  • Journal Title: Carcinogenesis Volume: 34 Issue: 6 Pages: 1251-1259
  • DOI: 10.1093/carcin/bgt053
  • Peer Reviewed
• [Journal Article] Circulating microRNAs in serum of human K-ras oncogene transgenic rats with pancreatic ductal adenocarcinomas (2012)
  • Author(s): Yabushita S, Fukamachi K, Tanaka H, Sumida K, Deguchi Y, Sukata T, Kawamura S, Uwagawa S, Suzui M, Tsuda H.
  • Journal Title: Pancreas Volume: 41 Issue: 7 Pages: 1013-1018
  • DOI: 10.1097/mpa.0b013e31824ac3a5
  • Peer Reviewed
• [Presentation] Identification of serum tumor marker of pancreas cancer by omics analysis (2014)
  • Author(s): Katsumi Fukamachi, Setsuko Yabushita, Mitsuru Futakuchi, Hiroyuki Tsuda, Masumi Suzui
  • Organizer: 発癌病理研究会
  • Place of Presentation: いわき市
  • Year and Date: 2014-09-03
• [Presentation] 活性型rasコンディショナルトランスジェニックラットを用いた膵がんモデル
  • Author(s): 深町勝巳、二口充、津田洋幸、酒々井眞澄
  • Organizer: 第26回発がん病理研究会
  • Place of Presentation: ルネッサンスサッポロホテル（札幌市）
• [Presentation] Rasトランスジェニックラットを用いた発がん研究
  • Author(s): 深町勝巳、二口充、津田洋幸、酒々井眞澄
  • Organizer: 平成23年度「個体レベルでのがん研究支援活動」ワークショップ(招待講演)
  • Place of Presentation: 琵琶湖ホテル（滋賀県）