Analysis of intracellular parasitism of Anaplasma phagocytophilum

• Project/Area Number: 23590514
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: University of Shizuoka
• Principal Investigator: OHASHI norio 静岡県立大学, 食品栄養科学部, 教授 (10169039)
• Co-Investigator(Kenkyū-buntansha): YOSHIKAWA Yuko 静岡県立大学, 食品栄養科学部, 助教 (00580523)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: アナプラズマ症 / 新興感染症 / A. phagocytophilum / 宿主感染応答 / 細胞内寄生性細菌

Outline of Final Research Achievements

Anaplasma phagocytophilum is an obligatory intracellular bacterium and causes human granulocytic anaplasmosis, an emerging tick-borne infectious disease. A. phagocytophilum forms characteristic inclusions in the host cells, so called morulae, and alters the host environment for the benefit of the bacterial multiplication. In the intracellular parasitism, we found that A. phagocytophilum activates three of all ER stress sensors, inositol-requiring enzyme 1α (IRE1α), protein kinase RNA-like ER kinase (PERK) and activating transcription factor-6 (ATF6), that regulate the eukaryotic unfolded protein response. The study suggests that A. phagocytophilum manipulates host ER stress system for intracellular survival and its growth.

Research Products

• [Journal Article] Molecular and serological survey of Rickettsiales bacteria in wild sika deer (Cervus nippon nippon) in Shizuoka prefecture, Japan: High prevalence of Anaplasma species (2015)
  • Author(s): Wu, D., Wuritu, Yoshikawa, Y., Gaowa, Kawamori, F., Ikegaya, A., Ohtake, M., Ohashi, M., Shimada, M., Takada, A., Iwai, K., and Ohashi, N.
  • Journal Title: Jpn. J. Infect. Dis. Volume: Advance Publication Issue: 5 Pages: 434-437
  • DOI: 10.7883/yoken.jjid.2015.003
  • NAID: 130005098926
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Anaplasma phagocytophilum antibodies in humans, Japan, 2010-2011. (2014)
  • Author(s): Gaowa, Yoshikawa, Y., Ohashi, N., Wu, D., Kawamori, F., Ikegaya, A., Watanabe, T., Saitoh, K., Takechi, D., Murakami, Y., Shichi, D., Aso, K., and Ando, S.
  • Journal Title: Emerging Infectious Diseases Volume: 20 Issue: 3 Pages: 508-509
  • DOI: 10.3201/eid2003.131337
  • Peer Reviewed / Open Access
• [Journal Article] Human granulocytic anaplasmosis in Japan. (2013)
  • Author(s): Ohashi, N., Gaowa, Wuritu, Kawamori, F., Wu, D., Yoshikawa, Y., Chiya, S., Fukunaga, K., Funato, T., Shiojiri, M., Nakajima, H., Hamauzu, Y., Takano, A., Kawabata, H., Ando, S., and Kishimoto, T
  • Journal Title: Emerging Infectious Diseases Volume: 19 Issue: 2 Pages: 289-292
  • DOI: 10.3201/eid1902.120855
  • Peer Reviewed
• [Journal Article] Rickettsiae in ticks, 2007-2011 Japan (2013)
  • Author(s): Gaowa, Ohashi, N., Aochi, M., Wuritu, Wu, D., Yoshikawa, Y., Kawamori, F., Honda, T., Fujita, H., Takada, N., Oikawa, Y., Kawabata, H., Ando, S., and Kishimoto, T.
  • Journal Title: Emerging Infectious Diseases Volume: 19 Issue: 2 Pages: 338-340
  • DOI: 10.3201/eid1902.120856
  • Peer Reviewed
• [Journal Article] Survey on Tick-Borne Pathogens in Thoroughbred Horses in the Hidaka District, Hokkaido, Japan (2013)
  • Author(s): Ybanez, A.P., Sato. F., Nambo. Y., Fukui. T., Masuzawa. T., Ohashi. N., Matsumoto. K., Kishimoto. T., and Inokuma, H.
  • Journal Title: Journal of Veterinary Medical Science Volume: 75 Issue: 1 Pages: 11-15
  • DOI: 10.1292/jvms.12-0282
  • NAID: 130001879687
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Detection and characterization of p44/msp2 transcript variants of Anaplasma phagocytophilum from naturally infected ticks and wild deer in Japan (2012)
  • Author(s): Gaowa, Wuritu, Wu, D., Yoshikawa, Y., Ohashi, N., Kawamori1, F., Sugiyama, K., Ohtake, M., Ohashi, M., Yamamoto, S., Kitano, T., Takada, N., Kawabata., H.
  • Journal Title: Jpn. J. Infect. Dis. Volume: 65 Pages: 79-83
  • Peer Reviewed
• [Journal Article] Molecular detection of Anaplasma phagocytophilum and Borrelia burgdorferi in Ixodes ricinus ticks from Istanbul metropolitan area and rural Trakya (Thrace) region of Northwestern Turkey (2011)
  • Author(s): Sen, E., Uchijima, Y., Kadosaka, T., Ohashi, N., Okamoto, Y., Fukui, T., Masuzawa, T.
  • Journal Title: Ticks Tick Borne Dis. Volume: 2 Pages: 94-98
  • Peer Reviewed
• [Journal Article] Detection of the new Ehrlichia species closely related to Ehrlichia ewingii from Haemaphysalis longicornis in Yonaguni Island, Okinawa, Japan (2011)
  • Author(s): Matsumoto, K., Takeuchi, T., Yokoyama, N., Katagiri, Y., Ooshiro, M., Zakimi, S., Gaowa, Kawamori, F., Ohashi, N., Inokuma, H.
  • Journal Title: J. Vet. Med. Sci. Volume: 73 Pages: 1485-1488
  • Peer Reviewed
• [Presentation] アナプラズマ症の特異抗体検出による患者探索の現状報告 (2014)
  • Author(s): 呉東興，高娃，吉川悠子，川森文彦，池ヶ谷朝香，川上万里，岸本壽男，森田裕司，中堂園文子，御供田睦代，能勢裕久，池田賢一，増澤俊幸，安藤秀二，大橋典男
  • Organizer: 第21回リケッチア研究会 研究発表会
  • Place of Presentation: 東京
  • Year and Date: 2014-12-20
• [Presentation] Anaplasma phagocytophilum感染患者血清中に存在する抗体の検出法に関する検討 (2014)
  • Author(s): 呉東興，高娃，吉川悠子，川森文彦，川上万理，岸本壽男，森田裕司，増澤俊幸，安藤秀二，大橋典男
  • Organizer: 第97回日本細菌学会関東支部総会
  • Place of Presentation: 東京
  • Year and Date: 2014-10-30 – 2014-10-31
• [Presentation] 日本国内に潜在する新興感染症「アナプラズマ症」 (2014)
  • Author(s): 大橋典男
  • Organizer: 平成26年度中部地区獣医師大会・獣医学術中部地区学会
  • Place of Presentation: 静岡
  • Year and Date: 2014-08-31
  • Invited
• [Presentation] Tick-associated Anaplasmataceae pathogens in Japan (2014)
  • Author(s): Ohashi N., Yoshikawa Y., Gaowa, Wuritu, Wu D., Kawamori F.
  • Organizer: XIV International Congress of Acarology
  • Place of Presentation: Kyoto
  • Year and Date: 2014-07-14 – 2014-07-18
  • Invited
• [Presentation] わが国の新興アナプラズマ症 (2014)
  • Author(s): 川森文彦, 大橋典男
  • Organizer: 衛生微生物技術協議会第35回研究会
  • Place of Presentation: 東京
  • Year and Date: 2014-06-26 – 2014-06-27
  • Invited
• [Presentation] アナプラズマ症の血清診断について (2014)
  • Author(s): 大橋典男、高娃、吉川悠子、呉東興、川森文彦、池ヶ谷朝香、渡邊卓哉、齊藤一仁、武地大維、村上陽一、志智大介、麻生克己、安藤秀二
  • Organizer: 第6回日本リケッチア症臨床研究会・第20回リケッチア研究会合同研究発表会
  • Place of Presentation: 大津
• [Presentation] ヒト顆粒球アナプラズマ症の疑いが持たれた1例 (2012)
  • Author(s): 村上陽一、志智大介、川森文彦、大橋典男
  • Organizer: 第217回日本内科学会東海地方会
  • Place of Presentation: 浜松
• [Presentation] One-tube nested PCR法による紅斑熱群リケッチアの検出 (2012)
  • Author(s): 川森文彦、池ヶ谷朝香、小柳純子、山田俊博、長岡宏美、大橋典男
  • Organizer: 第20回ダニと疾患のインタフェースに関するセミナー (SADI) 第20回記念大会
  • Place of Presentation: 阿南
• [Presentation] Overview of tick-associated Rickettsiales bacteria in Japan (2012)
  • Author(s): Ohashi, N., Yoshikawa, Y., Masuzawa, T.
  • Organizer: Russian Scientific Conference with International Participation “BASIC AND APPLIED ASPECTS OF INFECTIOUS PATHOLOGY”
  • Place of Presentation: Irkutsk (Russia)
• [Presentation] Comparative study of Anaplasma in Japan and other countries (2012)
  • Author(s): Masuzawa T., Ohashi, N., Khasnatinov, M.A., Danchinova, G.A., Ivanov, L.I., Fukui, T., Okamoto, Y., Pan, M.J., Koizumi, N.
  • Organizer: Russian Scientific Conference with International Participation “BASIC AND APPLIED ASPECTS OF INFECTIOUS PATHOLOGY”
  • Place of Presentation: Irkutsk (Russia)
• [Presentation] ヒト顆粒球アナプラズマ症が疑われた2例 (2012)
  • Author(s): 渡邊卓哉、武地大維、齊藤一仁、西尾信一郎、川森文彦、大橋典男
  • Organizer: 第55回日本感染症学会中日本地方学術集会
  • Place of Presentation: 福岡
• [Presentation] 近年のアナプラズマを中心としたリケッチア関連細菌群の調査概要 (2012)
  • Author(s): 大橋典男、高娃、呉東興、烏日図、吉川悠子、川森文彦
  • Organizer: 第5回日本リケッチア症臨床研究会・第19回リケッチア研究会合同研究発表会
  • Place of Presentation: 大津
• [Presentation] 発掘されつつあるアナプラズマ症やエーリキア症とその媒介動物 (2012)
  • Author(s): 大橋典男
  • Organizer: 第64回日本衛生動物学会大会
  • Place of Presentation: 上田市
• [Presentation] Human infection with Anaplasma phagocytophilum in Japan (2011)
  • Author(s): 高娃、烏日図、呉東興、古川英嗣、吉川悠子、川森文彦、大橋典男
  • Organizer: 第94回日本細菌学会関東支部総会
  • Place of Presentation: 東京
• [Presentation] Molecular epidemiological study of Rickettsiales bacteria in wild deer and mice in Shizuoka prefecture, Japan (2011)
  • Author(s): Wuritu, Wu D., Yoshikawa Y., Furukawa E., Murakami T., Minato C., Kawamori F., Ohtake M., Ohashi M., Ohashi N.
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌
• [Presentation] Molecular detection of Rickettsiales bacteria from ticks in western Japan (2011)
  • Author(s): Gaowa, Wuritu, Aochi M., Kawamori F., Yoshikawa Y., Ohashi N.
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌
• [Presentation] Rickettsiales bacteria in Japan (2011)
  • Author(s): Ohashi N.
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌