| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
We previously established a mouse model of AIP using chronic exposure to a commensal bacteria, Escherichia coli, leading to pancreatic inflammation, and elevation in IgG and IgG1 in serum and detection of anti-lactoferrin and anticarbonic anhydrace-II. The outer membrane fractions of E. coli were subjected to two-dimensional gel electrophoresis followed by immunoblotting against sera from the AIP model. One representative immunoreactive spot was determined using TOF/MS and Mascot search as FliC. Sera from patients with AIP had a signiticcantly higher antibody titer. Therefore, we are underway to clarify the possibility of a new diagnostic value of anti FliC-Ab titer.
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