| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
The basic protocols of FACS-mQ(mRNA quantification after FACS) were established. In FACS-mQ, intracellular RNAs are preserved without degradation after immunolabeling with a florophore, then we can analyze gene expression profiles of sorted cells after FACS. We established the following three protocols: 1)preparation of single cells from blood or tissue samples without RNA degradation, 2)high sensitive immunolabeling of the targeted cells, and 3) recovery and analysis of a samll amount of RNAs from sorted cells.
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