Development of high sensitivity assay for oxytocin, peptide hormone, and its clinical application utilizing aptamer

• Project/Area Number: 23590690
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Showa University
• Principal Investigator: SANO Yoshihiro 昭和大学, 薬学部, 講師 (40338538)
• Co-Investigator(Kenkyū-buntansha): ARAKAWA Hidetoshi 昭和大学, 薬学部, 教授 (70129807)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: アプタマー / オキシトシン / アスペルガー / 臨床分析化学 / ホルモン / 臨床薬学 / 臨床検査 / アスペルガー症候群 / ペプチドホルモン / 血中濃度測定 / 精神疾患

Research Abstract

Aptamers are single strand DNA and RNA oligonucleotides binding to their target with high affinity by three-dimensional conformation of aptamers. In this study, we report development of the high sensitively assay utilizing the aptamer for oxytocin, is associated to autism spectrum. As a result, various aptamers for oxytocin were generated by using RNA and DNA on SELEX. Those aptamers for oxytocin have checked to evaluate the affinity with oxytocin.

Research Products

• [Journal Article] Development of novel telomerase assay using PPDK-luciferin-luciferase detection system (2013)
  • Author(s): K. Karasawa, Y. Sano, H. Arakawa
  • Journal Title: Luminescence Volume: － Issue: 1 Pages: 52-57
  • DOI: 10.1002/bio.2501
  • Peer Reviewed
• [Journal Article] Development of RNA aptamer and its ligand binding assay on microchip electrophoresis (2012)
  • Author(s): K. Ohno, C. Nakata, Y. Sano, F. Nishikawa, S. Nishikawa and H. Arakawa
  • Journal Title: Current Chemical Genomics Volume: 6 Pages: 1-5
  • Peer Reviewed
• [Journal Article] Development of RNA Aptamer and its Ligand Binding Assay on Microchip Electrophoresis (2012)
  • Author(s): K. Ohno, C. Nakata, Y. Sano, F. Nishikawa, S. Nishikawa and H. Arakawa
  • Journal Title: Current Chemical Genomics Volume: 6 Pages: 1-5
  • Peer Reviewed
• [Journal Article] Bioluminescent assay for nitric oxide utilizing the biological enzyme activity of soluble guanylate cyclase (2011)
  • Author(s): Y. Sano, M. Seki, S. Abe, S. Suzuki and H. Arakawa
  • Journal Title: Analytical Letters Volume: 44(17) Pages: 2834-40
  • Peer Reviewed
• [Presentation] 血中Oxytocin測定を目的としたEIAの確立 (2013)
  • Author(s): 原谷汐美, 佐野佳弘, 大熊博, 荒川秀俊
  • Organizer: 第26回バイオメディカル分析科学シンポジウム
  • Place of Presentation: 東京
• [Presentation] Development of novel telomerase assay by bioluminescent detection method (2012)
  • Author(s): K. Karasawa, Y. Sano, H. Arakawa
  • Organizer: 17th International Symposium on Bioluminescence and Chemiluminescence (ISBC2012)
  • Place of Presentation: Guelph, Canada
• [Presentation] Oxytocin測定法の開発を目的としたアプタマーの創製 (2012)
  • Author(s): 佐野佳弘, 塚本啓貴, 荒川秀俊
  • Organizer: 日本薬学会132年会
  • Place of Presentation: 札幌
• [Presentation] 人工核酸抗体を用いたアプタマーアッセイ (2012)
  • Author(s): 荒川秀俊
  • Organizer: 生物化学的測定研究会第17回学術集会
  • Place of Presentation: 東京
• [Presentation] SELEX法によるオキシトシン反応性アプタマーの創製 (2012)
  • Author(s): 佐野佳弘, 伊與田佳介, 塚本啓貴, 荒川秀俊
  • Organizer: 第25回バイオメディカル分析科学シンポジウム
  • Place of Presentation: 東京
• [Presentation] SELEX法によるオキシトシン反応性アプタマーの創製 (2012)
  • Author(s): 佐野佳弘、伊與田佳介、塚本啓貴、荒川秀俊
  • Organizer: 第25回バイオメディカル分析科学シンポジウム 2012年8月 東京
  • Place of Presentation: 東京
• [Presentation] Oxytocin測定法の開発を目的としたアプタマーの創製 (2012)
  • Author(s): 佐野佳弘、塚本啓貴、荒川秀俊
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道大学
• [Presentation] 血中Oxytocin測定を目的とした生物発光Enzyme Immunoassayの確立 (2011)
  • Author(s): 佐野佳弘, 太田汐美, 荒川秀俊
  • Organizer: 生物発光化学発光研究会第28回学術講演会
  • Place of Presentation: 長崎
  • Year and Date: 2011-10-08
• [Presentation] 生物発光法を用いた新規テロメラーゼ分析法の開発 (2011)
  • Author(s): 唐沢浩二、佐野佳弘、荒川秀俊
  • Organizer: 生物発光化学発光研究会第28回学術講演会
  • Place of Presentation: 長崎
  • Year and Date: 2011-10-08
• [Presentation] Development of high sensitivity bioluminescent enzyme immunoassay for oxytocin as biomarkers for psychiatric disorder (2011)
  • Author(s): Yoshihiro Sano, Shiomi Ohta, Hiroshi Ohkuma, Haruhumi Tsuge and Hidetoshi Arakawa
  • Organizer: 2nd World Congress on Biomarkers & Clinical Research
  • Place of Presentation: Baltimore, USA
• [Remarks]
  • URL: http://www10.showa-u.ac.jp/~bachem/blankpage.html