New approach of benzodiazepine screening using QTOF method and techniques
| Project/Area Number |
23590859
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Koji 日本医科大学, 医学部, 助教 (90465344)
UEKUSA Kyoko 日本医科大学, 医学部, その他 (50409215)
KUDO Keiko 九州大学, 医学(系)研究科(研究院), 講師 (10186405)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 法医中毒学 / 質量分析 / 薬物分析 / ベンゾジアゼピン系 / QTOF / 薬物中毒 / ベンゾジアゼピン系薬物 / LC-MS-MS / 生体試料 |
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| Research Abstract |
The comprehensive screening techniques using the flight time type mass spectrometer (QTOF) are required for analysis of multiple-drug poisoning samples in postmortem examination. Application of the trigger MRM method of a benzodiazepines and other forensic toxicological drugs was tried. The simple ACN-deproteinization was enough as the pretreatent in this study. By conducting addition experiments of a target drug, precision of the transition for quantitation, ion suppression, etc. were considered. Reference library match score was high also with the postmortem cases as a result of the MS/MS spectrum library search. In this study, after performing noise rejection using the MFE method after QTOF measurement, the procedure of both a large scale database and a precision mass database library search was performed. As a result, some metabolites were detected and the concurrent use of QTOF/MFE and a precision mass database was effective as a comprehensive screening method.
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Report
(4 results)
Research Products
(80 results)
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[Presentation] 汎用型LCによるトリアゾラム,エチゾラム及びそれらの代謝物の迅速一斉分析2013
Author(s)
寺田賢,荒井裕子,佐々木千寿子,長谷川智華,林田眞喜子,田中榮之介,篠塚達雄,栗原克由,大野曜吉,黒崎久仁彦
Organizer
第97次日本法医学会学術全国集会
Place of Presentation
札幌
Related Report
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[Presentation] 汎用型LCによるトリアゾラム,エチゾラム及びそれらの代謝物の迅速一斉分析2013
Author(s)
寺田賢, 荒井裕子, 佐々木千寿子, 長谷川智華, 林田眞喜子, 田中榮之介, 篠塚達雄,栗原克由, 大野曜吉, 黒崎久仁彦.
Organizer
第97次日本法医学会学術全国集会
Place of Presentation
札幌市
Related Report
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[Presentation] Rapid screening and semi-quantitative analysis for forensic drugs in blood using liquid chromatography triple quadrupole mass spectrometry.2013
Author(s)
Thomas H, Kudo K, Minohata T, Usui K, Shima N, Katagi M, Tsuchihashi H, Suzuki K, Hirano I, Ikeda N.
Organizer
61th ASMS Conference on Mass Spectrometry; Abstracts.
Place of Presentation
Minneapolis, Minnesota, USA
Related Report
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[Presentation] Screening analysis for drugs of abuse by LC-MS/MS enables fast polarity switching MRM2012
Author(s)
Minohata T, Hirano I, Iida J, Kudo K, Ikeda N, Zaitsu K, Shima N, Katagi M, Tsuchihashi K, Suzuki K.
Organizer
19th International Mass Spectrometry Conference
Place of Presentation
Kyoto, Japan
Related Report
Invited
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