| Project/Area Number |
23590859
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Koji 日本医科大学, 医学部, 助教 (90465344)
UEKUSA Kyoko 日本医科大学, 医学部, その他 (50409215)
KUDO Keiko 九州大学, 医学(系)研究科(研究院), 講師 (10186405)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 法医中毒学 / 質量分析 / 薬物分析 / ベンゾジアゼピン系 / QTOF / 薬物中毒 / ベンゾジアゼピン系薬物 / LC-MS-MS / 生体試料 |
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| Research Abstract |
The comprehensive screening techniques using the flight time type mass spectrometer (QTOF) are required for analysis of multiple-drug poisoning samples in postmortem examination. Application of the trigger MRM method of a benzodiazepines and other forensic toxicological drugs was tried. The simple ACN-deproteinization was enough as the pretreatent in this study. By conducting addition experiments of a target drug, precision of the transition for quantitation, ion suppression, etc. were considered. Reference library match score was high also with the postmortem cases as a result of the MS/MS spectrum library search. In this study, after performing noise rejection using the MFE method after QTOF measurement, the procedure of both a large scale database and a precision mass database library search was performed. As a result, some metabolites were detected and the concurrent use of QTOF/MFE and a precision mass database was effective as a comprehensive screening method.
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