| Project/Area Number |
23590935
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KURIYAMA Shigeru 浜松医科大学, 医学部, 助教 (90596446)
SUGIMOTO Ken 浜松医科大学, 医学部, 准教授 (20529507)
IWAIZUMI Moriya 浜松医科大学, 医学部, 助教 (60444361)
濱屋 寧 浜松医科大学, 医学部, 助教 (20436968)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 潰瘍性大腸炎 / 糞便 / 糞便RNA発現 / 糞便RNA検査 / 疾患活動性 / RNA |
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| Research Abstract |
Fecal CD45 or proinflammatory cytokines mRNA were evaluated as a noninvasive biomarker of disease activity or mucosal inflammation for ulcerative colitis (UC). Total 86 colonoscopies were performed in 73 UC patients. Samples from feces and blood were analyzed for fecal mRNA assay and laboratory findings (CRP, ESR, Hb, Plt, Alb), and the results were compared with colonoscopic findings. Mucosal status was assessed using the Mayo endoscopic score (0-3). There was significant differences in all non-invasive markers and CAI among the Mayo endoscopic score (p < 0.01). Endoscopic disease activity correlated closest with CAI, followed by fecal mRNA assay, Alb, CRP, ESR, Hb, and Plt. In addition, nineteen of 42 patients with Mayo 2 or 3 had CAI less than 5, however, more than half of 19 patients had positive result of fecal mRNA assay when proper cutoff was set. These results suggested that fecal mRNA assay is useful as a noninvasive biomarker of mucosal inflammation for UC.
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