| Project/Area Number |
23590995
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MOGUSHI Kaoru 順天堂大学, 医学(系)研究科, 助教 (60569292)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 肝発癌 / 酸化ストレス / 肝腫瘍原性遺伝子 / 網羅的遺伝子発現解析 / mRNA / microRNA / siRNA / 網羅的解析 |
|---|
| Outline of Final Research Achievements |
Persistent liver injury caused by various etiology is involved in hepatocarcinogenesis, which develops through different pathways. Since the most common pathogenesis is persistent exposure to oxidative stress, oxidative stress-induced gene could be one of the most basic hepatocarcinogenic genes. IQGAP1 knockdown resulted in reduced activities of migration and invasion in some hepatoma cell lines, but not in others. Cells with high IQGAP1 expression levels were poorly-differentiated carcinomas with high migration/invasion activities. These cell properties were closely related to IQGAP1. Vimentin also played a critical role in the properties of some cell lines, indicating that knockdown of genes specific to each cancer cell is required to reduce the migration and invasion activities. Furthermore, IQGAP1 with a tumor promotion activity could have an inhibitory activity against tumor growth, suggesting that IQGAP1 has conflicting properties in a cell- and/or microRNA-dependent manner.
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