Epigenetic control of cardiac gene expressions by non-canonical Wnts

• Project/Area Number: 23591083
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Kyushu University
• Principal Investigator: KOYANAGI Masamichi 九州大学, 大学病院, 助教 (00325474)
• Co-Investigator(Kenkyū-buntansha): MAEDA Toyoki 九州大学, 大学病院, 准教授 (30264112)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: エピジェネテックス / 再生医療 / エピジェネティック / Wntシグナル / 国際情報交換（ドイツ） / 循環器内科学

Research Abstract

It has been shown that canonical Wnt as well as non-canonical Wnt signal is important to induce cardiac genes. However underlying signal is not well elucidated. We found that non-canonical Wnt5a increase histone lysine methylases and demethylases in endothelial progenitor cells. This increases changed the pattern of methylated histone. These epigenetic changes has been found in hematopoietic stem progenitor cells in human, mouse, and rats, but not on ES cells and MSCs so far.

Research Products

• [Journal Article] Alterations in the telomere length distribution and the subtelomeric methylation status in human vascular endothelial cells under elevated temperature in culture condition. (2013)
  • Author(s): Maeda T, Guan JZ, Koyanagi M, Makino N.
  • Journal Title: Aging Clin Exp Res. Volume: 25 Issue: 3 Pages: 231-238
  • DOI: 10.1007/s40520-013-0045-6
  • Peer Reviewed
• [Journal Article] Acute myocardial infarction activates progenitor cells and increases Wnt signaling in the bone marrow (2012)
  • Author(s): Assmus B, Iwasaki M, Schächinger V, Roexe T, Koyanagi M, Iekushi K, Xu Q, Tonn T, Seifried E, Liebner S, Kranert WT, Gruenwald F, Dimmeler S, Zeiher AM
  • Journal Title: Eur Heart J Volume: 33(15) Issue: 15 Pages: 1911-9
  • DOI: 10.1093/eurheartj/ehr388
  • Peer Reviewed
• [Journal Article] Aging-associated alteration of telomere length and subtelomeric status in female patients with Parkinson's disease. (2012)
  • Author(s): Maeda T, Guan JZ, Koyanagi M, Higuchi Y, Makino N.
  • Journal Title: Journal of Neurogenetics Volume: 26 Issue: 2 Pages: 245-251
  • DOI: 10.3109/01677063.2011.651665
  • Peer Reviewed
• [Journal Article] Upregulation of anticoagulant proteins, protein S and tissue factor pathway inhibitor, in the mouse myocardium with cardio-specific TNF-alpha overexpression (2012)
  • Author(s): Higuchi Y, Kubota T, Koyanagi M, Maeda T, Feldman AM, Makino N.
  • Journal Title: Am J Physiol Heart Circ Physiol Volume: 302(11) Issue: 11 Pages: H2352-H2362
  • DOI: 10.1152/ajpheart.01026.2011
  • Peer Reviewed
• [Journal Article] Epigenetic Regulation of Endothelial Lineage Committed Genes in Pro-Angiogenic Hematopoietic and Endothelial Progenitor Cells (2011)
  • Author(s): Ohtani, K; Vlachojannis, GJ; Koyanagi, M et al.
  • Journal Title: CIRCULATION RESEARCH Volume: 109巻11号 Issue: 11 Pages: 1219-1229
  • DOI: 10.1161/circresaha.111.247304
• [Journal Article] Hepatocyte Growth Factor Induces a Proangiogenic Phenotype and Mobilizes Endothelial Progenitor Cells by Activating Nox2 (2011)
  • Author(s): Schroder, K; Schutz, S; Schloffel, I; Batz, S; Takac, I; Weissmann, N; Michaelis, UR; Koyanagi, M; Brandes,RP
  • Journal Title: ANTIOXIDANTS & REDOX SIGNALING Volume: 15巻4号 Issue: 4 Pages: 915-923
  • DOI: 10.1089/ars.2010.3533
• [Journal Article] Epigenetic repression of endothelial lineage committed genes in pro- angiogenic hematopoietic progenitor cells (2011)
  • Author(s): Ohtani K, Vlachojannis GJ, Koyanagi M, Boeckel JN, Urbich C, Marquez VE, Zeiher AM, & Dimmeler S
  • Journal Title: Circ Res Volume: 109(11) Pages: 1219-29
  • Peer Reviewed
• [Presentation] Title;Epigenetic control of stem cell function (2012)
  • Author(s): Masamichi Koyanagi
  • Organizer: AHA Scientific Sessions
  • Place of Presentation: Los Angels, USA
  • Invited
• [Presentation] Epigenetic control of stem cell function (2012)
  • Author(s): Masamichi Koyanagi
  • Organizer: AHA Scientific Sessions
  • Place of Presentation: Los Angels, USA
  • Invited
• [Presentation] Title;Wnt5a increases histone lysine demethylases and induces epigenetic remodeling (2011)
  • Author(s): Masamichi Koyanagi
  • Organizer: European Epigenomics & Stem Cells-2011 Meetings
  • Place of Presentation: Paris
• [Presentation] Wnt5a increases histone lysine demethylases and induces epigenetic remodeling (2011)
  • Author(s): Masamichi Koyanagi
  • Organizer: European Epigenomics & Stem Cells-2011 Meetings(招待講演)
  • Place of Presentation: Paris
• [Book] Title; Circulating progenitor cells a part of"HEART REGENERATION Stem Cells and Beyond" (2012)
  • Author(s): Masamichi Koyanagi and Stefanie Dimmeler
  • Publisher: Pub.