Identification of microRNAs involved in the development of diabetic nephropathy

• Project/Area Number: 23591177
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Akita University
• Principal Investigator: FUJITA HIROKI 秋田大学, 医学部, 講師 (30333933)
• Co-Investigator(Kenkyū-buntansha): YAMADA Yuichiro 秋田大学, 大学院医学系研究科, 教授 (60283610)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 糖尿病性腎症 / マイクロRNA

Research Abstract

MicroRNAs (miRs) are a family of non-coding RNAs and inhibit target gene expression by causing messenger RNA degradation or by blocking protein translation. We analysed the specific expression of miRs by microarray and real-time RT-PCR using RNA extracted from whole kidneys and isolated glomeruli in two Akita diabetic mouse models which have different susceptibility to diabetic nephropathy, nephropathy-resistant C57BL/6-Akita and nephropathy-prone KK/Ta-Akita. Several miRs whose expression levels are different between C57BL/6-Akita and KK/Ta-Akita were identified in both whole kidney and glomerulus. These results indicate that the identified miRs may play an important role in the development and progression of diabetic nephropathy.

Research Products

• [Presentation] マイクロアレイ解析による糖尿病性腎症感受性マイクロRNAの同定 (2011)
  • Author(s): 藤田浩樹、森井宰、藤田麻貴、高嶋悟、小川和孝、三ケ田敦史、菅沼由美、清水尚子、清水辰徳、佐藤雄大、細葉美穂子、月山克史、成田琢磨、山田祐一郎
  • Organizer: 第54回日本糖尿病学会年次学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2011-05-20
• [Presentation] マイクロアレイ解析による糖尿病性腎症感受性マイクロRNAの同定 (2011)
  • Author(s): 藤田浩樹、森井宰、藤田麻貴、高嶋悟、小川和孝、三ケ田敦史、菅沼由美、清水尚子、清水辰徳、佐藤雄大、細葉美穂子、月山克史、成田琢磨、山田祐一郎
  • Organizer: 第54回日本糖尿病学会年次学術集会
  • Place of Presentation: 札幌プリンスホテル国際館パミール
• [Remarks]
  • URL: http://www.med.akita-u.ac.jp