Adipose-derived mesenchymal stem cells transplantation facilitate experimental peritoneal fibrosis repair by suppressing epithelial–mesenchymal transition
Project/Area Number |
23591226
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Kidney internal medicine
|
Research Institution | Juntendo University |
Principal Investigator |
HAMADA Chieko 順天堂大学, 医学部, 准教授 (50291662)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | peritoneal fibrosis / mesenchymal stem cell / EMT / angiogenesis / 腹膜線維化 / 脂肪幹細胞 / 上皮間葉系移行 / 血管新生 / 肪幹細胞 / 被嚢性腹膜硬化症 / VEGF / TNF-α / IL-1β / 再生医療 / HGF / basic FGF |
Research Abstract |
Peritoneal fibrosis (PF) remains a serious complication of PD that involves in the morphological and functional deteriorations of peritoneum. It is still unclear the effect of adipose-derived mesenchymal stem cell (ASC) to the PF. The effect of ASC transplantation was compared with peritoneal mesothelial cell(PMC) during repair in chlorhexidine gluconate (CG)-induced PF rats. To induce the PF rat, continuous-infusion pumps containing 8 % CG were placed into the abdominal cavity for 21 days. PMCs and ASCs were injected at day 22 or 29, and sacrificed at day 35. ASC transplantation significantly facilitated peritoneal repair in the dose dependent manner regardless of transplant timing. The mRNA expression of fibrosis-related factors were significantly suppressed. ASC transplantation induced the over-expression of angiogenesis-related factors. It appears that the ASC might be an effective strategy for morphological retrieval in the PF through the anti-inflammatory and anti-EMT effect.
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Report
(4 results)
Research Products
(5 results)