| Project/Area Number |
23591302
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Nagoya University |
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Principal Investigator |
BANNO Ryoichi 名古屋大学, 医学部附属病院, 助教 (80597865)
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| Co-Investigator(Kenkyū-buntansha) |
OISO Yutaka 名古屋大学, 大学院医学系研究科, 教授 (40203707)
ARIMA Hiroshi 名古屋大学, 大学院医学系研究科, 准教授 (50422770)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 肥満 / 視床下部炎症 / PTP1B / エネルギーバランス |
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| Research Abstract |
Hypothalamus is well known to play an important role in the regulation of energy balance. High-fat diet (HFD) increases hypothalamic Protein tyrosine phosphatase 1B (PTP1B) expression levels, and hypothalamic PTP1B deficient models protect against diet induced obesity. While PTP1B has therefore been implicated as a key regulator of energy homeostasis, the factors regulating PTP1B expression in the hypothalamus, particularly under obese conditions, remain to be fully elucidated. Since several lines of evidence have suggested that HFD induces hypothalamic inflammation accompanied by increasing levels of inflammatory cytokines such as TNF-alpha and IL6, in addition to ER stress, we examined whether these factors could regulate PTP1B expression and activity by using hypothalamic organotypic cultures. Our data show that TNF-alpha significantly increases PTP1B expression and activity in the hypothalamus, and that these effects are mediated via TNF-alpha induced NF-kappa-B activation.
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