Nobel quantification system of non-transferrin-bound iron utilizing automated analyzer
Project/Area Number |
23591363
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Hematology
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Research Institution | Asahikawa Medical College |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
IKUTA Katsuya 旭川医科大学, 医学邪, 講師 (00396376)
OHTAKE Takaaki 旭川医科大学, 医学邪, 特任教授 (10359490)
KOHGO Yutaka 旭川医科大学, 医学邪, 教授 (10133183)
TANAKA Hiroki 旭川医科大学, 医学邪, 特任助教 (70596155)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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Keywords | 鉄 / 不安定鉄 / NTBI / 鉄過剰 / トランスフェリン / HPLC / 自動分析装置 / バイオマーカー / NTA / キレート剤 / クロマトグラフ用樹脂剤 / 鉄代謝 |
Research Abstract |
Iron overload causes various organ damages. When transferrin is fully saturated, non-transferrin-bound iron (NTBI) appears in the serum. Since NTBI relates to oxidant stress, monitoring serum NTBI levels is crucial for iron-overloaded patients. In the present study, we aimed to develop the novel NTBI measuring reagent corresponding to the automatic analyzer by modifying the high-performance liquid chromatography (HPLC)-based NTBI assay (the gold standard method). The correlation coefficient for NTBI values between our system and HPLC method was 0.923, indicating that the accuracy of our system is comparable to the gold standard method. Our novel NTBI assay system should be a powerful tool for studying physiological and clinical importance of NTBI.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Hoerferritinemia after adult allogeneic hematopoietic cell transplantation : quantification of iron burden by determining non-transferrin-bound iron2013
Author(s)
Tatsunori Goto, Katsuya Ikuta, Yoshihiro Inamoto, Sonoko Kamoshita, Emi Yokohata, Daisuke Koyama, Koichi Onodera, Aika Seto, Keisuke Watanabe, Nobuhiko Imahashi, Shokichi Tsukamoto, Yukiyasu Ozawa, Katsunori Sasaki, Masafumi Ito, Yutaka Kohgo and Koichi Miyamura
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Journal Title
Int J Hematol
Volume: 97
Pages: 125-134
Related Report
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[Journal Article] Hyperferritinemia after adult allogeneic hematopoietic cell transplantation: quantification of iron burden by determining non-transferrin-bound iron.2013
Author(s)
Tatsunori Goto, Katsuya Ikuta, Yoshihiro Inamoto, Sonoko Kamoshita, Emi Yokohata, Daisuke Koyama, Koichi Onodera, Aika Seto, Keisuke Watanabe, Nobuhiko Imahashi, Shokichi Tsukamoto, Yukiyasu Ozaw, Katsunori Sasaki, Masafumi Ito, Yutaka Kohgo, Koichi Miyamura
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Journal Title
Int J Hematol.
Volume: 97
Issue: 1
Pages: 125-134
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Iron overload signature in chrysotile-induced malignant mesothelioma2012
Author(s)
Li Jiang, Shinya Akatsuka, Yoriko Yamashita, Hirotak Nagai, Hiroki Ohara, Shan-Hwu Chew, Yasumasa Okazaki, Yutak Yoshikawa, Hiroyuki Yasui, Katsuya Ikuta, Katsunori Sasaki, Yutaka Kohgo, Seishiro Hirano, Yasushi Shinohara, Norihiko Kohyama, Takashi Takahashi, Shinya Toyokuni
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Journal Title
J Pathol
Volume: 228
Pages: 366-377
Related Report
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[Journal Article] Iron overload signature in chrysotile-induced malignant mesothelioma.2012
Author(s)
Jiang L, Akatsuka S, Nagai H, Chew SH, Ohara H, Okazaki Y, Yamashita Y, Yoshikawa Y, Yasui H, Ikuta K, Sasaki K, Kohgo Y, Hirano S, Shinohara Y, Kohyama N, Takahashi T, Toyokuni S.
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Journal Title
J Pathol
Volume: 228
Issue: 3
Pages: 366-77
DOI
Related Report
Peer Reviewed
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[Presentation] Nobel quantification system of non-transferrin-bound iron (NTBI) utilizing automatic analyzer2013
Author(s)
Katsuya Ikuta, Satoshi Ito, Lynda Addo, Mayumi Hatayama, Yasumichi Toki, Junki Inamura, Motohiro Shindo, Hiroki Tanaka, Katsunori Sasaki, Yoshihiro Torimoto, Yutaka Kohgo
Organizer
2013 IBIS Meeting BioIron
Place of Presentation
London, UK
Related Report
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[Presentation] Nobel quantification system for non-transferrin-bound iron utilizing automated analyzer2013
Author(s)
Junki Inamura, Katsuya Ikuta, Daisuke Kato, Naomi Iizuka, Yasumichi Toki, Satoshi Ito, Hiroki Tanaka, Katsunori Sasaki, Motohiro Shindo, Yoshihiro Torimoto, Yutaka Kohgo
Organizer
第75回日本血液学会学術集会(JSH2013)
Place of Presentation
札幌
Related Report
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[Presentation] Nobel quantification system for non-transferrin-bound iron utilizing automated analyzer2013
Author(s)
Junki Inamura, Katsuya Ikuta, Daisuke Kato, Naomi Iizuka, Yasumichi Toki, Satoshi Ito, Hiroki Tanaka, Katsunori Sasaki, Motohiro Shindo, Yoshihiro Torimoto, Yutaka Kohgo
Organizer
第75回日本血液学会学術集会(JSH2013)
Place of Presentation
札幌
Related Report
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