| Project/Area Number |
23591363
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IKUTA Katsuya 旭川医科大学, 医学邪, 講師 (00396376)
OHTAKE Takaaki 旭川医科大学, 医学邪, 特任教授 (10359490)
KOHGO Yutaka 旭川医科大学, 医学邪, 教授 (10133183)
TANAKA Hiroki 旭川医科大学, 医学邪, 特任助教 (70596155)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 鉄 / 不安定鉄 / NTBI / 鉄過剰 / トランスフェリン / HPLC / 自動分析装置 / バイオマーカー / NTA / キレート剤 / クロマトグラフ用樹脂剤 / 鉄代謝 |
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| Research Abstract |
Iron overload causes various organ damages. When transferrin is fully saturated, non-transferrin-bound iron (NTBI) appears in the serum. Since NTBI relates to oxidant stress, monitoring serum NTBI levels is crucial for iron-overloaded patients. In the present study, we aimed to develop the novel NTBI measuring reagent corresponding to the automatic analyzer by modifying the high-performance liquid chromatography (HPLC)-based NTBI assay (the gold standard method). The correlation coefficient for NTBI values between our system and HPLC method was 0.923, indicating that the accuracy of our system is comparable to the gold standard method. Our novel NTBI assay system should be a powerful tool for studying physiological and clinical importance of NTBI.
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