Establishment of a patient-derived iPS cells for simulating myelodysplastic syndrome

• Project/Area Number: 23591373
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: The University of Tokyo
• Principal Investigator: TOJO Arinobu 東京大学, 医科学研究所, 教授 (00211681)
• Co-Investigator(Renkei-kenkyūsha): KOBAYASHI Seiichiro 東京大学医科学研究所, 分子療法分野, 助教 (70376622) ; IZAWA Kiyoko 東京大学医科学研究所, 分子療法分野, 特任研究員 (20534415)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: iPS細胞 / 骨髄異形成症候群（MDS） / 初期化因子 / レンチウイルスベクター / センダイウイルスベクター / CD34+細胞 / 骨髄異形成症候群 / CD34陽性細胞 / 細胞初期化 / 遺伝子変異 / MDS / レンチウイルス / Cre/loxP

Research Abstract

Lentiviral vectors expressing 4 reprogramming factors (Oct4, Sox3, Klf4 and c-Myc) driven by constitutive (EF1a) or inducible (Tet-on) promoter were newly prepared. We tried to reprogram CD34+ cells from 5 patients with MDS (RCMD 3, RARS 1, and MDS/MPD 1) as well as 1 patient with CML-CP by infection with either lentiviral vector. In each case, a number of cell mass resembling iPSC colony appeared over the MEF layer within 3 weeks of culture, but, unfortunately, none of these iPSC-like colonies developed stable cell lines. Then, we took alternative method using a Sendai virus that does not integrate into the host genome. As a result, a patient-derived iPSCs were successfully established from a male patient with RCMD at the very end of the research periods. Since the patient's bone marrow cells revealed normal karyotype, comparative genetic examination between the patient's granulocytes (MDS clone), T cells (normal clone) and iPSCs are required to identify the true origin of iPSCs.

Research Products

• [Journal Article] CADM1 expression and stepwise down-regulation of CD7 is closely associated with clonal selection of HTLV-1-infected T cells potentially evolving into adult T cell leukemia/lymphoma (2014)
  • Author(s): Kobayashi S, Nakano K, Watanabe E, Ishigaki T, Ohno N, Yuji K, Oyaizu N, Asanuma S, Yamagishi M, Yamochi T, Watanabe N, Tojo A, Watanabe T, Uchimaru K
  • Journal Title: Clin Cancer Res Volume: 20(11) Pages: 2851-2861
• [Journal Article] Stage-Specific Embryonic Antigen 4 is not a marker for proliferation and pluripotency in Wharton's Jelly-derived mesenchymal stem cells (2014)
  • Author(s): He H, Nagamura-Inoue T, Tsunoda H, Yuzawa M, Yamamoto Y, Yorozu P, Tojo A
  • Journal Title: Tissue Eng Part A Volume: 20(7-8) Pages: 1314-24
• [Journal Article] CADM1 expression and stepwise downregulation of CD7 are closely associated with clonal expansion of HTLV-1-infected cells in adult T-cell leukemia/lymphoma. (2014)
  • Author(s): Kobayashi S, Tojo A, et al.
  • Journal Title: Clin. Cancer Res Volume: 20
  • Peer Reviewed
• [Journal Article] The use of bone marrow stromal cells (bone marrow-derived multipotent mesenchymal stromal cells) for alveolar bone tissue engineering: basic science to clinical translation. (2014)
  • Author(s): Kagami H, Tojo A, et al.
  • Journal Title: Tissue Eng. Part B. Volume: 20
  • NAID: 120006986575
  • Peer Reviewed
• [Journal Article] Stage-Specific Embryonic Antigen 4 is not a marker for proliferation and pluripotency in Wharton’s Jelly-derived mesenchymal stem cells. (2014)
  • Author(s): He H, Tojo A, et al.
  • Journal Title: Tissue Eng Part A. Volume: 20 Issue: 7-8 Pages: 1314-1324
  • DOI: 10.1089/ten.tea.2013.0333
  • Peer Reviewed / Open Access
• [Journal Article] Impact of sex incompatibility on the outcome of single-unit cord blood transplantation for adult patients with hematological malignancies. (2014)
  • Author(s): Konuma T, Tojo A, et al.
  • Journal Title: Bone Marrow Transplant. Volume: 49 Issue: 5 Pages: 634-9
  • DOI: 10.1038/bmt.2014.10
  • Peer Reviewed / Open Access
• [Journal Article] Pretransplant hyperferritinemia has no effect on the outcome of myeloablative cord blood transplantation for acute leukemia and myelodysplastic syndrome. (2014)
  • Author(s): Konuma T, Tojo A, et al.
  • Journal Title: Ann Hematol. Volume: 93 Issue: 6 Pages: 1071-1072
  • DOI: 10.1007/s00277-013-1932-9
  • Peer Reviewed
• [Journal Article] Effect of ABO Blood Group Incompatibility on the Outcome of Single-Unit Cord Blood Transplantation after Myeloablative Conditioning (2014)
  • Author(s): Konuma T, Tojo A, et al.
  • Journal Title: Biol Blood Marrow Transplant. Volume: 20 Issue: 4 Pages: 577-581
  • DOI: 10.1016/j.bbmt.2013.12.563
  • Peer Reviewed / Open Access
• [Journal Article] Single-Unit Cord Blood Transplantation after Granulocyte Colony-Stimulating Factor-Combined Myeloablative Conditioning for Myeloid Malignancies Not in Remission. (2014)
  • Author(s): Konuma T, Tojo A, et al.
  • Journal Title: Biol Blood Marrow Transplant. 20(3): Volume: 20 Issue: 3 Pages: 396-401
  • DOI: 10.1016/j.bbmt.2013.12.555
  • Peer Reviewed
• [Journal Article] In vivo leukemogenic potential of an interleukin-7 receptor-a mutant in hematopoietic stem/progenitor cells (2013)
  • Author(s): Yokoyama K, Yokoyama N, Izawa K, Kotani A, Harashima A, Hozumi K, Tojo A
  • Journal Title: Blood Volume: 22(26) Pages: 4259-63
• [Journal Article] miR-126-mediated control of cell fate in B cell-myeloid progenitors as a potential alternative to transcriptional factors (2013)
  • Author(s): Okuyama K, Ikawa T, Harnprasopwat R, Lu J, Yamashita R, Ha D, Toyoshima T, Chanda B, Kawamata T, Yokoyama K, Gertner B, Wang S, Ando K, Lodish HF, Tojo A, Kawamoto H, Kotani A
  • Journal Title: Proc Natl Acad Sci USA Volume: 110(33) Pages: 13410-5
• [Journal Article] A versatile drug delivery system using streptavidin-tagged pegylated liposomes and biotinylated biomaterials (2013)
  • Author(s): Chen MH, Soda Y, Izawa K, Kobayashi S, Tani K, Maruyama K, Tojo A, Asano S
  • Journal Title: Int J Pharm Volume: 454(1) Pages: 478-85
• [Journal Article] The CD3 versus CD7 plot in multicolor flow cytometry reflects progression of disease stage in patients infected with HTLV-I (2013)
  • Author(s): Kobayashi S, Tian Y, Ohno N, Yuji K, Ishigaki T, Isobe M, Tsuda M, Oyaizu N, Watanabe E, Watanabe N, Tani K, Tojo A, Uchimaru K
  • Journal Title: PLoS One Volume: 8(1)
• [Journal Article] In vivo leukemogenic potential of an interleukin-7 receptor-α mutant in hematopoietic stem/progenitor cells. (2013)
  • Author(s): Yokoyama K, Tojo A, et al.
  • Journal Title: Blood Volume: 122 Issue: 26 Pages: 4259-4263
  • DOI: 10.1182/blood-2012-08-451278
  • Peer Reviewed
• [Journal Article] Quantitative PCR detection of CEP110-FGFR1 fusion gene in a patient with 8p11 syndrome. (2013)
  • Author(s): Yamamoto S, Ebihara Y, Mochizuki S, Kawakita T, Kato S, Ooi J, Takahashi S, Tojo A, Yusa N, Furukawa Y, Oyaizu N, Watanabe J, Sato K, Kimura F, Tsuji K.
  • Journal Title: Leukemia and Lymphoma Volume: 54 Issue: 9 Pages: 2068-2069
  • DOI: 10.3109/10428194.2013.767455
  • Peer Reviewed
• [Journal Article] MicroRNA-126-mediated control of cell fate in B-cell myeloid progenitors as a potential alternative to transcriptional factors. (2013)
  • Author(s): Okuyama K, Ikawa T, Gentner B, Hozumi K, Harnprasopwat R, Lu J, Yamashita R, Ha D, Toyoshima T, Chanda B, Kawamata T, Yokoyama K, Wang S, Ando K, Lodish HF, Tojo A, Kawamoto H, Kotani A
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 110 Issue: 33 Pages: 13410-13415
  • DOI: 10.1073/pnas.1220710110
  • Peer Reviewed
• [Journal Article] Ischemic culture of dental pulp-derived cells is a useful model in which to investigate mechanisms of post-ischemic tissue recovery. (2013)
  • Author(s): Agata A, Tojo A, et al.
  • Journal Title: Histol Histopathol. Volume: 28 Pages: 985-991
  • Peer Reviewed
• [Journal Article] Management of adult Langerhans cell histiocytosis based on the characteristic clinical features. (2013)
  • Author(s): Imashuku S, Tojo A, et al.
  • Journal Title: World J Hematol. Volume: 2 Pages: 89-98
  • Peer Reviewed
• [Journal Article] Unusual extramedullary relapse after haploidentical bone marrow transplantation in a patient with acute lymphoblastic leukemia. (2013)
  • Author(s): Ebihara Y, Tojo A, et al.
  • Journal Title: J Blood Disorders Transf. Volume: 4 Pages: 5-5
  • Peer Reviewed
• [Journal Article] Loss of CCR4 antigen expression after mogamulizumab therapy in a case of adult T-cell leukaemia-lymphoma. (2013)
  • Author(s): Ohno N, Tojo A, et al.
  • Journal Title: Br J Haematol. Volume: 163 Issue: 5 Pages: 683-685
  • DOI: 10.1111/bjh.12555
  • Peer Reviewed
• [Journal Article] Development of peripheral T-cell lymphoma not otherwise specified in an HTLV-1 carrier. (2013)
  • Author(s): Ishigaki T, Isobe M, Kobayashi S, Yuji K, Ohno N, Watanabe N, Tojo A and Uchimaru K.
  • Journal Title: Int J Heamtol. Volume: prepublished online Issue: 5 Pages: 667-672
  • DOI: 10.1007/s12185-013-1314-z
  • NAID: 10031176497
  • Peer Reviewed
• [Journal Article] Association of hepatitis B with antirheumatic drugs: a case-control study. (2013)
  • Author(s): Oshima Y, Tojo A, et al.
  • Journal Title: Mod Rheumatol. Volume: 23 Issue: 4 Pages: 694-704
  • DOI: 10.1007/s10165-012-0709-7
  • NAID: 10031189163
  • Peer Reviewed
• [Journal Article] An association between acute myelogenous leukemia and thrombopoietin receptor agonist in immune thrombocytopenia patients. (2013)
  • Author(s): Oshima Y, Tojo A, et al.
  • Journal Title: Int Med. Volume: 52 Pages: 2193-2201
  • Peer Reviewed
• [Journal Article] Acute kidney injury after myeloablative cord blood transplantation in adults: the efficacy of strict monitoring of vancomycin serum trough concentrations. (2013)
  • Author(s): Mae H, Tojo A, et al.
  • Journal Title: Transplant Infect Dis. Volume: 15 Issue: 2 Pages: 181-6
  • DOI: 10.1111/tid.12038
  • Peer Reviewed
• [Journal Article] Effect of administration of reprogramming factors in the mouse liver. (2013)
  • Author(s): Tomokuni A, Tojo A, et al.
  • Journal Title: Oncol Lett. Volume: 6 Pages: 323-8
  • Peer Reviewed
• [Journal Article] Pneumothorax in an early phase after allogeneic hematopoietic stem cell transplantation. (2013)
  • Author(s): Ebihara Y, Tojo A, et al.
  • Journal Title: Hematol Rep. Volume: 5 Issue: 2 Pages: 34-35
  • DOI: 10.4081/hr.2013.e10
  • Peer Reviewed
• [Journal Article] Feasibility of autologous bone marrow mesenchymal stem cells cultured with autologous serum for treatment of haemophilic arthropathy. (2013)
  • Author(s): Ebihara Y, Takedani H, Ishige I, Nagamura-Inoue T, Wakitani S, Tojo A, Tsuji K.
  • Journal Title: Haemophilia. Volume: 19 Issue: 2
  • DOI: 10.1111/hae.12056
  • Peer Reviewed
• [Journal Article] A versatile drug delivery system using streptavidin-tagged pegylated liposomes and biotinylated biomaterials. (2013)
  • Author(s): Chen MH, Tojo A, et al.
  • Journal Title: Int J Pharm. Volume: 454 Issue: 1 Pages: 478-485
  • DOI: 10.1016/j.ijpharm.2013.06.031
  • Peer Reviewed / Open Access
• [Journal Article] The CD3 versus CD7 plot in multicolor flow cytometry reflects progression of disease stage in patients infected with HTLV-I. (2013)
  • Author(s): Kobayashi S, Tian Y, Ohno N, Yuji K, Ishigaki T, Isobe M, Ohfuchi-Tsuda M, Oyaizu N, Watanabe E, Watanabe N, Tani K, Tojo A and Uchimaru K.
  • Journal Title: PLoS One Volume: 8 Issue: 1 Pages: e53728-e53728
  • DOI: 10.1371/journal.pone.0053728
  • Peer Reviewed
• [Journal Article] Therapeutic outcome of multifocal Langerhans cell histiocytosis in adults treated with the Special C regimen formulated by the Japan LCH Study Group. (2013)
  • Author(s): Morimoto A, Shimazaki C, Takahashi S, Yoshikawa K, Nishimura R, Wakita H, Kobayashi Y, Kanegane H, Tojo A, Imamura T, Imashuku S; Japan LCH Study Group.
  • Journal Title: Int J Hematol. Volume: 97 Issue: 1 Pages: 103-108
  • DOI: 10.1007/s12185-012-1245-0
  • NAID: 10031144641
  • Peer Reviewed
• [Journal Article] ErbB/NF-κB signaling controls mammosphere formation in human breast cancer (2012)
  • Author(s): Hinohara K, Kobayashi S, Kanauchi H, Simizu S, Nishioka K, Tsuji E, Tada K, Umezawa K, Mori M, Ogawa T, Inoue J, Tojo A, Gotoh N
  • Journal Title: Proc Natl Acad Sci USA Volume: 109 Pages: 6584-9
• [Journal Article] Imatinib mesylate directly impairs class switch recombination through downregulation of AID (2012)
  • Author(s): Kawamata T, Jun L, Sato T, Tanaka M, Nagaoka H, Agata Y, Toyoshima T, Yokoyama K, Oyaizu N, Nakamura N, Ando K, Tojo A, Kotani A
  • Journal Title: Blood Volume: 119 Pages: 3123-7
• [Journal Article] ETV6-NTRK3 as a therapeutic target of small molecule inhibitor PKC412. (2012)
  • Author(s): Chi HT, Ly BT, Kano Y, Tojo A, Watanabe T, Sato Y.
  • Journal Title: Biochem Biophys Res Commun. Volume: 429 Issue: 1-2 Pages: 87-92
  • DOI: 10.1016/j.bbrc.2012.10.087
  • Peer Reviewed
• [Journal Article] Acute lymphoblastic leukemia with t(1;19)(q23;p13)/TCF3-PBX1 fusion in an adult male with Down Syndrome. (2012)
  • Author(s): Yamamoto S, Ebihara Y, Mochizuki S, Tsuda M, Yuji K, Uchimaru K, Tojo A, Tsuji K.
  • Journal Title: Acta Haematol. Volume: 128 Issue: 4 Pages: 242-243
  • DOI: 10.1159/000340049
  • Peer Reviewed
• [Journal Article] Characteristic difference in the composition of osteogenic cell populations of BMSCs isolated from untreated, hemolysed, or Ficoll-treated rat bone marrow (2012)
  • Author(s): Agata H, Yamazaki M, Uehara M, Hori A, Sumita Y, Tojo A, Kagami H
  • Journal Title: Cytotherapy Volume: vol.14(7) Issue: 7 Pages: 791-801
  • DOI: 10.3109/14653249.2012.674639
  • Peer Reviewed
• [Journal Article] ErbB/NF-kB signaling controls mammosphere formation in human breast cancer (2012)
  • Author(s): Hinohara, K.
  • Journal Title: Proc.Natl.Acad.Sci Volume: (in press) Issue: 17 Pages: 6584-6589
  • DOI: 10.1073/pnas.1113271109
  • Peer Reviewed
• [Journal Article] Efficacy and safety of nilotinib in Japanese patients with imatinib-resistant or -intolerant Ph+ CML or relapsed/refractory Ph+ ALL: a 36-month analysis of a phase I and II study. (2012)
  • Author(s): Usuki K, et al
  • Journal Title: Int J Hematol Volume: 95 Issue: 4 Pages: 409-419
  • DOI: 10.1007/s12185-012-1026-9
  • Peer Reviewed
• [Journal Article] Imatinib mesylate directly impairs class switch recombination through downregulation of AID. (2012)
  • Author(s): Kawamata T, Lu J, Sato T, Tanaka M, Nagaoka H, Agata Y, Toyoshima T, Yokoyama K, Oyaizu N, Nakamura N, Ando K, Tojo A, Kotani A
  • Journal Title: Blood Volume: 119 Issue: 13 Pages: 3123-3127
  • DOI: 10.1182/blood-2011-01-327932
  • Peer Reviewed
• [Journal Article] Helicobacter pylori-induced thrombocytosis clinically indistinguishable from essential thrombocythemia. (2012)
  • Author(s): Kawamata T, Tojo A.
  • Journal Title: Leuk Lymphoma. Volume: 53 Issue: 7 Pages: 1423-1424
  • DOI: 10.3109/10428194.2011.653787
  • Peer Reviewed
• [Journal Article] Unrelated cord blood transplantation after myeloablative conditioning regimen in adolescent and young adult patients with hematologic malignancies : a single institute analysis (2012)
  • Author(s): Ebihara, Y., et al.
  • Journal Title: Leuk.Res. Volume: 36 Issue: 2 Pages: 128-131
  • DOI: 10.1016/j.leukres.2011.09.016
  • Peer Reviewed
• [Journal Article] Pulmonary alveolar hemorrhage possibly associated with lenalidomide use. (2012)
  • Author(s): Oshima Y, Tojo A.
  • Journal Title: Int J Hematol. Volume: 94 Issue: 3 Pages: 296-297
  • DOI: 10.1007/s12185-011-0915-7
  • NAID: 10029694439
  • Peer Reviewed
• [Journal Article] Bioimaging analysis of NF-kB activity in Ph-positive acute lymphoblastic leukemia cells unveils its synergistic up-regulation by TNFa-stimulated changes to the microenvironment (2011)
  • Author(s): Tsai HJ, Kobayashi S, Izawa K, Ishida T, Watanabe T, Umezawa K, Lin SF, Tojo A
  • Journal Title: Cancer Sci Volume: 102 Pages: 2014-21
• [Journal Article] Gaussia luciferase for bioluminescence tumor monitoring in comparison with firefly luciferase (2011)
  • Author(s): Inoue Y, Sheng F, Kiryu S, Watanabe M, Harnprasopwat R, Izawa K, Tojo A, Ohtomo K
  • Journal Title: Mol Imaging Volume: 10 Pages: 377-85
• [Journal Article] Bioimaging analysis of NF-κB activity in Ph-positive acute lymphoblastic leukemia cells unveils its synergistic up-regulation by TNFα-stimulated changes to the microenvironment. (2011)
  • Author(s): Tsai HJ, Kobayashi S, Izawa K, Ishida T, Watanabe T, Umezawa K, Lin SF, Tojo A.
  • Journal Title: Cancer Science Volume: 102 Issue: 11 Pages: 2014-2021
  • DOI: 10.1111/j.1349-7006.2011.02039.x
  • Peer Reviewed
• [Journal Article] Gaussia luciferase for bioluminescence tumor monitoring in comparison with firefly luciferase. (2011)
  • Author(s): Inoue Y, Sheng F, Kiryu S, Watanabe M, Harnprasopwat R, Izawa K, Tojo A, Ohtomo K.
  • Journal Title: Molecular Imaging. Volume: 10 Issue: 5 Pages: 377-385
  • DOI: 10.2310/7290.2010.00057
  • Peer Reviewed
• [Journal Article] Leukemic T cells are specifically enriched in a unique CD3dimCD7low subpopulation of CD4+ T cells in acute-type adult T cell leukemia. (2011)
  • Author(s): 14.Tian Y, Kobayashi S, Ohno N, Isobe M, Tsuda M, Zaike Y, Watanabe N, Tojo A, Tani K, Uchimaru K.
  • Journal Title: Cancer Science Volume: 102 Issue: 3 Pages: 569-577
  • DOI: 10.1111/j.1349-7006.2010.01833.x
  • Peer Reviewed
• [Presentation] マイクロRNA制御性ワクシニアウイルスは多発性骨髄腫細胞選択的に細胞死をもたらす (2013)
  • Author(s): 三見宗孔、中村貴文、東保有伸
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] Mantle cell lymphoma with hypersensitivity to mosquito bites in the elderly : a distinct entity Mantle cell lymphoma with hypersensitivity to mosquito bites in the elderly : a distinct entity (2013)
  • Author(s): 湯地晃一郎、佐藤広太、城憲秀、小林真之、磯部優理、島田直樹、石橋通宏、小沼貴品、大野伸広、小林誠一郎、小柳津直樹、内丸薫、東保有伸
  • Organizer: 第75回日本血液学会学術集会
  • Place of Presentation: 札幌
• [Presentation] Marked Eosinophilia Caused by lnterleukin 5 producing Cardiac Myxoma (2013)
  • Author(s): 佐藤広太、湯地晃一郎、津田真由子、大野伸広、内丸薫、東保有伸
  • Organizer: 第75回日本血液学会学術集会
  • Place of Presentation: 札幌
• [Presentation] Clinical profile of adult Langerhans cell histiocytosis : A single institute experience in Japan (2013)
  • Author(s): 小林真之、佐藤広太、川俣豊隆、湯地晃一郎、大野伸広、高橋聡、内丸薫、東保有伸
  • Organizer: 第75回日本血液学会学術集会
  • Place of Presentation: 札幌
• [Presentation] SSEA4 is not a marker for proliferation and pluripotency in Wharton's Jelly derived MSCs (2013)
  • Author(s): Haiping He、長村登紀子、角田肇、湯沢美紀、山本由紀、東保有伸
  • Organizer: 第75回日本血液学会学術集会
  • Place of Presentation: 札幌
• [Presentation] The immnosuppressive effect of Wharton Jelly-derived mesenchymal stem cells in vitro (2013)
  • Author(s): He H, Nagamura-Inoue T, Yamamoto Y, Mori Y, Tsunoda H, Tojo A
  • Organizer: The 55th American Society of Hematology Annual Meeting and Exposition
  • Place of Presentation: New Orleans, USA
• [Presentation] Long-term ex vivo maintenance of murine iPSC-derived hematopoietic stem/progenitor cells by conditional HoxB4 (2013)
  • Author(s): Izawa K, Yamamoto M, Tojo A
  • Organizer: The 55th American Society of Hematology Annual Meeting and Exposition
  • Place of Presentation: New Orleans, USA
• [Presentation] miRNA-regulated vaccinia viru selectively targets multiple myeloma. (2013)
  • Author(s): 二見宗孔、中村貴史、東條有伸
  • Organizer: 第72回日本癌学会学術集会
  • Place of Presentation: 横浜市
• [Presentation] The Impact of severe chronic GVHD on survival after cord blood transplantation. (2013)
  • Author(s): 加藤せい子、山口拓洋、大井 淳、河北敏郎、塚田瑞夫、東條有伸、高橋 聡
  • Organizer: 第75回日本血液学会学術集会
  • Place of Presentation: 札幌市
• [Presentation] lmpairment of T cell development in chronic myeloid leukemia,partial explanation by in vitro model (2012)
  • Author(s): Chanda Bidisha、東保有伸、他
  • Organizer: 第74回血液学会学術集会
  • Year and Date: 2012-10-20
• [Presentation] CD7 vs CADM1 in FACS reflects multi step oncogenesis of ATL and discriminates HTLV 1 infected cells (2012)
  • Author(s): 小林誠一郎、東保有伸、他
  • Organizer: 第74回血液学会学術集会
  • Place of Presentation: (口演)
  • Year and Date: 2012-10-19
• [Presentation] Characterization of stem cell in human umbilical cord derived mesenchymal stem cells (2012)
  • Author(s): 何海葎、東保有伸、他
  • Organizer: 第74回血液学会学術集会
  • Place of Presentation: (ポスター)
  • Year and Date: 2012-10-19
• [Presentation] Impairment of T cell development in chronic myeloid leukemia, partial explanation by in vitro model. (2012)
  • Author(s): 1. Chanda Bidisha、伊澤清子、Harnprasopwat Ratanakanit、小林誠一郎、東條有伸.
  • Organizer: 第74回日本血液学会学術集会
  • Place of Presentation: 京都
• [Presentation] New insights into Bcr-Abl-mediated transformation of hematopoietic cells using regulatable Bcr-Abl (2011)
  • Author(s): Tojo A
  • Organizer: The seasonal combined conference of the Hematology Society and Blood and BM transplantation Society of Taiwan
  • Place of Presentation: Kaohsiung, Taiwan(Keynote Speech)
  • Year and Date: 2011-12-12
• [Presentation] Bcr-Abl impairs T cell development from murine induced pluripotent stem cells and hematopoietic stem cells; a partial explanation for the concept that Ph+ clone never involves T cell lineage in CML (2011)
  • Author(s): Bidisha C, Izawa K, Harnprasopwat R, Takahashi K, Kobayashi S, Kanegae Y, Saito I, Tojo A
  • Organizer: 53rd ASH (American Society of Hematology) Annual Meeting and Exposition
  • Place of Presentation: San Diego, USA(Poster Presentation)
  • Year and Date: 2011-12-12
• [Presentation] The in vitro and in vivo oncogenic activity of homodimeric mutant of interleukin-7 receptor a chain (IL7Rα) highlights the significance of the IL7Rα/Jak1 pathway in T-cell acute lymphoblastic leukemia (2011)
  • Author(s): Yokoyama K, Yokoyama N, Izawa K, Kotani A, Harnprasopwat R, Harashima A, Kobayashi S, Tojo A
  • Organizer: 53rd American Society of Hematology (ASH) Annual Meeting and Exposition
  • Place of Presentation: San Diego, USA(Oral Presentation)
  • Year and Date: 2011-12-11
• [Presentation] CMLイマチニフ治療の現状と展望 (2011)
  • Author(s): 東保有伸
  • Organizer: 第73回日本血液学会学術集会
  • Place of Presentation: 名古屋(教育講演)
  • Year and Date: 2011-10-16
• [Presentation] Bcr-Abl impairs T cell development from murine induced pluripotent stem cells; a possible explanation for T cell escape from leukemic clone in chronic myeloid leukemia (2011)
  • Author(s): Bidisha C, Izawa K, Harnprasopwat R, Takahashi K, Kobayashi S, Kanegae Y, Saito I, Tojo A
  • Organizer: 9th ISSCR (International Society for Stem Cell Research) Annual Meeting
  • Place of Presentation: Toronto, Canada(Poster Presentation)
  • Year and Date: 2011-06-16
• [Presentation] 造血幹細胞移植医療の最先端と課題 (2011)
  • Author(s): 東保有伸
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜(ワークショップ13)
  • Year and Date: 2011-04-30
• [Presentation] Bcr-Abl impairs T cell development from murine induced pluripotent stem cells and hematopoietic stem cells; a partial explanation for the concept that Ph+ clone never involves T cell lineage in CML. (2011)
  • Author(s): Bidisha C, Izawa K, Harnprasopwat R, Takahashi K, Kobayashi S, Kanegae Y, Saito I, Tojo A.
  • Organizer: 53rd ASH (American Society of Hematology) Annual Meeting and Exposition.
  • Place of Presentation: San Diego, USA
• [Presentation] Unique CD7lo CADM1+ T cell population in leukemogenesis by HTLV-1.
  • Author(s): Tojo A, Kobayashi S, Ohno N, Ishigaki T, Watanabe N, Watanabe T, Uchimaru K.
  • Organizer: XXVI Symposium International Association for Comparative Research on Leukemia and Related Diseases (IACRLRD)
  • Place of Presentation: Turin, Italy
  • Invited
• [Presentation] The impact of severe chronic GVHD on survival after cord blood transplantation.
  • Author(s): Kato S, Yamaguchi T, Ooi J, Tsukada M, Kawakita T, Tojo A, Takahashi S.
  • Organizer: 39th Annual Meeting of the European Group of Blood and Marrow Transplantation.
  • Place of Presentation: London, GB
• [Presentation] The immnosuppressive effect of Wharton Jelly-derived mesenchymal stem cells in vitro.
  • Author(s): He H, Nagamura-Inoue T, Yamamoto Y, Mori Y, Tsunoda H, Tojo A.
  • Organizer: The 55th American Society of Hematology Annual Meeting and Exposition.
  • Place of Presentation: New Orleans, USA
• [Presentation] Long-term ex vivo maintenance of murine iPSC-derived hematopoietic stem/progenitor cells by conditional HoxB4. The 55th American Society of Hematology Annual Meeting and Exposition.
  • Author(s): Izawa K, Yamamoto M, Tojo A.
  • Organizer: The 55th American Society of Hematology Annual Meeting and Exposition.
  • Place of Presentation: New Orleans, USA
• [Book] 「血液疾患 最新の治療2014-2016」主な血液疾患用薬剤の作用機序と適応『II-2. 造血因子』 (2014)
  • Author(s): 東條有伸（分担執筆）直江知樹・小澤敬也・中尾真二編
  • Total Pages: 373
  • Publisher: 南山堂
• [Book] イマチニブ「抗がん剤の臨床薬理」 (2013)
  • Author(s): 東保有伸(相羽恵介編)
  • Publisher: 南山堂
• [Book] 造血因子「血液疾患最新の治療2014-2016」 (2013)
  • Author(s): 東保有伸(直江知樹、小津敬也、中尾真三編)
  • Publisher: 南江堂
• [Book] 序にかえて~G-CSFの発見から臨床応用へ~ 「G-CSFの基礎と臨床」 (2013)
  • Author(s): 東保有伸 (東保有伸編)
  • Publisher: 医薬ジャーナル社
• [Book] G-CSFの基礎と臨床 (2013)
  • Author(s): 東條有伸（編著）
  • Total Pages: 191
  • Publisher: 医薬ジャーナル社
• [Book] 「抗がん剤の臨床薬理」第III章 分子標的薬の各論 2. 小分子化合物 1)グリベック (2013)
  • Author(s): 東條有伸(分担執筆) 相羽恵介編
  • Total Pages: 731
  • Publisher: 南山堂