The cytotoxic effect by natural killer cells against pediatric malignant solid tumors

Research Project

Project/Area Number	23591532
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Pediatrics
Research Institution	University of Yamanashi
Principal Investigator	GOI Kumiko 山梨大学, 医学部附属病院, 講師 (70324192)
Project Period (FY)	2011 – 2013
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000) Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	NK細胞 / 小児固形腫瘍 / 同種移植 / GVT / 小児難治性固形腫瘍 / GVT効果 / 抗腫瘍効果
Research Abstract	We analyzed the effect and the mechanisms of the cytotoxicity of NK cells against pediatric solid tumors. The surface expressions of HLA class I as ligand of NK inhibitory receptors in neuroblastoma cell lines were significantly lower than ones in the other tumor cell lines. The expressions of the ligands of NK activating receptor were also low in neuroblastoma cells, but slightly high in some other tumor cells. We confirmed the cytotoxicities of NK cells against the neuroblastoma and malignant rhabdoid tumor. However, the effects by the mismatch of HLA-C group between donor NK cells and target tumor cells were not significant in this cytotoxicities.