Effectiveness for islet isolation by hypothermic machine perfusion presevation of pancreas

• Project/Area Number: 23591887
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Clinical research Center, Chiba-East National Hospital, National Hospital Organization
• Principal Investigator: AKUTSU Naotake 独立行政法人国立病院機構(千葉東病院臨床研究センター), その他部局等, その他 (00344979)
• Co-Investigator(Kenkyū-buntansha): OTSUKI Kazunori 国立病院機構千葉東病院, 臨床研究部, 室長 (50399755) ; MARUYAMA Michihiro 国立病院機構千葉東病院, 診療部長 (40399754) ; KENMOCHI Takashi 藤田保健衛生大学, 医学部臓器移植科, 教授 (50215133) ; ITO Taihei 藤田保健衛生大学, 医学部臓器移植科, 准教授 (60509701)
• Co-Investigator(Renkei-kenkyūsha): SAIGO Kenichi 国立病院機構千葉東病院, 診療部長 (60323424) ; HASEGAWA Masayuki 国立病院機構千葉東病院, 外科医長 (80648030) ; AOYAMA Hiromichi 国立病院機構千葉東病院, 外科医師 (80598243) ; MATSUMOTO Ikuko 国立病院機構千葉東病院, 外科医師 (70649128) ; NOGUCHI Hirofumi 琉球大学, 医学部再生医学講座, 教授 (50378733)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 膵島移植 / 臓器保存 / 持続冷却潅流保存 / 膵臓保存 / 移植・再生医療 / 移植 / 膵ランゲルハンス島（膵島） / 灌流保存

Outline of Final Research Achievements

Hypothermic machine perfusion preservation (HMP) by LifePortTM is thought to be a better preservation technique for pancreas harvested from marginal donors such as non-heart-beating donor. In this experiment, we indicated that yield and function of islet isolated from 24hours-preserved canine pancreas with LifePortTM removed after 30 minutes warm ischemic time. It suggests that HMP for pancreas would be a useful method for islet transplantation from marginal donors in Japan.

Research Products

• [Journal Article] 持続灌流装置（LifePort)による障害腎・膵の保存・搬送に関する実験的研究 (2011)
  • Author(s): 圷 尚武 他
  • Journal Title: Organ Biology Volume: 18 Pages: 81-86
  • NAID: 130004709987
• [Presentation] 心停止下膵島移植に向けたLifePortによる持続冷却潅流保存の検討 (2015)
  • Author(s): 圷尚武
  • Organizer: 第42回日本膵･膵島移植研究会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-06 – 2015-03-07
• [Presentation] 膵島移植に向けた採取膵持続潅流保存の検討 (2014)
  • Author(s): 圷尚武
  • Organizer: 第41回日本臓器保存生物医学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2014-11-28 – 2014-11-29
• [Presentation] Hypotheramic machine perfusion preservation is efficient for non-heart-beating donor kidney transplantation in Japan (2012)
  • Author(s): Akutsu N, Kenmochi T, Saitoh T, Maruyama M, Iwashita C, Otsuki K, Ito T, Matsumoto I, Asano T
  • Organizer: 24th International Congress of the Transplantation Society
  • Place of Presentation: Berlin