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The role of proton-sensing G-protein coupled receptors(GPCRs) in the regulation of inflammatory cytokine production during endoscopic surgery

Research Project

Project/Area Number 23591916
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General surgery
Research InstitutionGunma Institute of Public Health and Environmental Sciences

Principal Investigator

MURATA Naoya  群馬県衛生環境研究所, 研究企画係, 研究員 (00533473)

Co-Investigator(Kenkyū-buntansha) OKAJIMA Fumikazu  群馬大学, 生体調節研究所, 教授 (30142748)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsプロトン感知性受容体 / OGR1受容体ファミリー / TDAG8 / 抗炎症性応答 / 実験外科学
Research Abstract

The role of proton-sensing GPCRs in the regulation of lipopolysaccharide(LPS)-induced inflammatory cytokine production was investigated. In vivo injection of LPS into mice resulted in the remarkable accumulation of TNF-alpha. The LPS-induced action tends to be enhanced in the mice deficient in TDAG8 or GPR4; however, the effects of receptor deficiency were not significant. Extracellular acidification inhibited LPS-induced TNF-alpha protein and mRNA production in mouse peritoneal macrophages in vitro. Our results suggest that acidic pH inhibits inflammatory cytokine production at least partly through TDAG8/cAMP/PKA pathways in mouse macrophages.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (32 results)

All 2014 2013 2012 2011

All Journal Article (14 results) (of which Peer Reviewed: 14 results,  Open Access: 1 results) Presentation (18 results)

  • [Journal Article] Lipoprotein-associated lysolipids are differentially involved in high-density lipoprotein- and its oxidized form-induced neurite remodeling in PC12 cells.2014

    • Author(s)
      Koichi Sato, Masayuki Tobo, Chihiro Mogi, Naoya Murata, Mie Kotake, Atsushi Kuwabara, Dong-Soon Im, Fumikazu Okajima
    • Journal Title

      Neurochemistry International

      Volume: 68 Pages: 38-47

    • DOI

      10.1016/j.neuint.2014.02.005

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of interleukin-1β production by extracellular acidification through the TDAG8/cAMP pathway in mouse microglia.2014

    • Author(s)
      Ye Jin, Koichi Sato, Ayaka Tobo, Chihiro Mogi, Masayuki Tobo, Naoya Murata, Satoshi Ishii, Dong-Soon Im and Fumikazu Okajima
    • Journal Title

      Journal of Neurochemistry

      Volume: 129(4) Issue: 4 Pages: 683-695

    • DOI

      10.1111/jnc.12661

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Role of extracellular proton-sensing receptors in regulation of insulin secretion and pancreaticβ−cell functions2014

    • Author(s)
      Mogi C, Nakakura T, and Okajima F.
    • Journal Title

      Endocrine J.

      Volume: 61 Pages: 101-110

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of inflammation by extracellular acidification and proton-sensing GPCRs2013

    • Author(s)
      Okajima F.
    • Journal Title

      Cellular Signalling

      Volume: 25 Issue: 11 Pages: 2263-2271

    • DOI

      10.1016/j.cellsig.2013.07.022

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Oxidative stress potentially enhances FcεRI-mediated leukotriene C_4 release dependent on the late-phase increase of intracellular glutathione in mast cells2013

    • Author(s)
      Seki K, Hisada T, Kawata T, Kamide Y, Dobashi K, Yamada M, Mori M, Okajima F, Ishizuka T
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 439 Issue: 3 Pages: 357-362

    • DOI

      10.1016/j.bbrc.2013.08.081

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lysophosphatidylethanolamine utilizes LPA1 and CD97 in MDA-MB-231 breast cancer cells2013

    • Author(s)
      Park SJ, Lee KP, Kang S, Chung HY, Bae YS, Okajima F, and Im DS.
    • Journal Title

      Cellular Signalling

      Volume: 25 Issue: 11 Pages: 2147-2154

    • DOI

      10.1016/j.cellsig.2013.07.001

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proton-sensing ovarian cancer G protein-coupled receptor 1 on dendritic cells is required for airway responses in a murine asthma model2013

    • Author(s)
      Aoki H, Mogi C, Hisada T, Kamide Y, Ichimonji I, Tomura H, Tobo M, Sato K, Tsurumaki H, Dobashi K, Mori T, Harada H, Yamada M, Mori M, Ishizuka T, Okajima F
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 11 Pages: e79985-e79985

    • DOI

      10.1371/journal.pone.0079985

    • URL

      https://pure.teikyo.jp/en/publications/43d0a4de-500b-4215-8c43-216e5af1efa1

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ovarian cancer G-protein-coupled receptor 1 induces the expression of the pain mediator prostaglandin E2 in response to an acidic extracellular environment in human osteoblast-like cells2012

    • Author(s)
      Wang J, Sun Y, Tomura H, Okajima F.
    • Journal Title

      Int J Biochem Cell Biol.

      Volume: 44 Issue: 11 Pages: 1937-1941

    • DOI

      10.1016/j.biocel.2012.07.015

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Deficiency of proton-sensing ovarian cancer G protein-coupled receptor 1 attenuates glucose- stimulated insulin secretion2012

    • Author(s)
      Nakakura T
    • Journal Title

      Endocrinology

      Volume: 153 Issue: 9 Pages: 4171

    • DOI

      10.1210/en.2012-1164

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] An orally active lysophosphatidic acid receptor antagonist attenuates panereatic cancer invasion and metastasis in vivo2012

    • Author(s)
      Komachi M
    • Journal Title

      Cancer Science

      Volume: (印刷中) Issue: 6 Pages: 1099-1104

    • DOI

      10.1111/j.1349-7006.2012.02246.x

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of proton-sensing receptor TDAG8 in the anti-inflammatory actions of dexamethasone in peritoneal macrophages2011

    • Author(s)
      Xiao-Dong He
    • Journal Title

      Biochem.Biophys.Res.Commun.

      Volume: 415 Issue: 4 Pages: 627

    • DOI

      10.1016/j.bbrc.2011.10.122

    • URL

      https://pure.teikyo.jp/en/publications/c087b552-5841-417c-8f3d-ea0c37fa47c1

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Extracellular acidification induces connective tissue growth factor production through proton-sensing receptor OGR1 in human airway smooth musele cells2011

    • Author(s)
      Matsuzaki S
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 413 Issue: 4 Pages: 499-503

    • DOI

      10.1016/j.bbrc.2011.08.087

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Constitutively active heat shock factor 1 enhances glucose-driven insulin secretion2011

    • Author(s)
      Uchiyama T
    • Journal Title

      Metabolism

      Volume: 60 Issue: 6 Pages: 789-798

    • DOI

      10.1016/j.metabol.2010.07.029

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Unmasking of LPA(1)receptor-mediated migration response to lysophosphatidic acid by interleukin-1β-induced attenuation of Rho signaling pathways in rat astrocytes2011

    • Author(s)
      Sato K
    • Journal Title

      J Neurochem

      Volume: 117 Issue: 1 Pages: 164-174

    • DOI

      10.1111/j.1471-4159.2011.07188.x

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] プロトン感知性受容体OGR1の気管支喘息における役割2013

    • Author(s)
      青木悠、茂木千尋、久田剛志、鶴巻寛朗、上出庸介、中倉敬、笠原礼光、西岡正樹、関香織、矢冨正清、小野昭浩、古賀康彦、土橋邦生、石塚全、山田正信、岡島史和
    • Organizer
      第63回日本アレルギー学会秋季学術大会
    • Place of Presentation
      ホテルニューオオタニ(東京)
    • Related Report
      2013 Final Research Report
  • [Presentation] N1E115神経細胞における細胞外酸性pHによるcGMP産生機構2013

    • Author(s)
      小竹美絵、佐藤幸市、岡島史和
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Related Report
      2013 Final Research Report
  • [Presentation] Follow-up of reactivation of HB virus in HBV antibody positive lymphoma after chemotherapy in a single insutitution2013

    • Author(s)
      五十嵐忠彦、村山佳予子、入沢寛之、村田直哉
    • Organizer
      第11回日本臨床腫瘍学会学術集会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Related Report
      2013 Final Research Report
  • [Presentation] 化学療法後に甲状腺の再腫大を認めた甲状腺原発悪性リンパ腫の2例2013

    • Author(s)
      村山佳予子、入沢寛之、村田直哉、五十嵐忠彦
    • Organizer
      第53回日本リンパ網内系学会総会
    • Place of Presentation
      国立京都国際会館(京都)
    • Related Report
      2013 Final Research Report
  • [Presentation] 化学療法後に甲状腺の再腫大を認めた甲状腺原発悪性リンパ腫の2例2013

    • Author(s)
      村山佳予子, 入沢寛之, 村田直哉, 五十嵐忠彦
    • Organizer
      第53回日本リンパ網内系学会総会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Follow-up of reactivation of HB virus in HBV antibody positive lymphoma after chemotherapy in a single institution2013

    • Author(s)
      五十嵐忠彦, 村山佳予子, 入沢寛之, 村田直哉
    • Organizer
      第11回日本臨床腫瘍学会学術集会
    • Place of Presentation
      仙台国際センタ-(宮城県仙台市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] プロトン感知性OGR1ファミリ-GPCRとその機能2012

    • Author(s)
      岡島史和
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2013 Final Research Report
  • [Presentation] 膵β細胞におけるプロトン感知性OGR1を介したインスリン分泌シグナルの解析2012

    • Author(s)
      中倉敬、茂木千尋、戸村秀明、岡島史和
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2013 Final Research Report
  • [Presentation] Involvement of TDAG8 in extracellular acidification-induced inhibition of interleukin-1beta production in microglia2012

    • Author(s)
      Ye Jin, Koichi Sato, Fumikazu Okajima
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2013 Final Research Report
  • [Presentation] N1E115神経細胞における細胞外酸性pHによる細胞内Ca2+動員、Aktリン酸化とプロトン感知性受容体の関与2012

    • Author(s)
      佐藤幸市、小竹美絵、岡島史和
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2013 Final Research Report
  • [Presentation] プロトンはマウス肥満細胞からのサイトカイン産生を調節する2012

    • Author(s)
      上出庸介、石塚全、鶴巻寛朗、山下均、山田秀典、宇津木光克、小野昭浩、古賀康彦、久田剛志、土橋邦生、岡島史和、森昌朋
    • Organizer
      第62回日本アレルギー学会秋季学術大会
    • Place of Presentation
      大阪国際会議場(大阪)
    • Related Report
      2013 Final Research Report
  • [Presentation] プロトン感知性Gタンパク質共役型受容体OGR1KOマウスにおける気管支喘息モデルの解析2012

    • Author(s)
      青木悠、茂木千尋、久田剛志、石塚全、上出庸介、鶴巻寛朗、土橋邦生、岡島史和、森昌朋
    • Organizer
      第62回日本アレルギー学会秋季学術大会
    • Place of Presentation
      大阪国際会議場(大阪)
    • Related Report
      2013 Final Research Report
  • [Presentation] Primary malignant lymphoma of the breast presenting a huge inflammatory breast mass2012

    • Author(s)
      Tadahiko Igarashi, Kayoko Murayama, Hiroyuki Irisawa, Naoya Murata, Masaru Kojima, Yasuhiro Yanagida
    • Organizer
      第74回日本血液学会学術集会
    • Place of Presentation
      国立京都国際会館(京都)
    • Related Report
      2013 Final Research Report
  • [Presentation] Incidence and outcome of the infectious diseases after MEAM therapy with autoPBSCT for lymphoma2012

    • Author(s)
      Kayoko Murayama, Hiroyuki Irisawa, Naoya Murata, Tadahiko Igarashi
    • Organizer
      第74回日本血液学会学術集会
    • Place of Presentation
      国立京都国際会館(京都)
    • Related Report
      2013 Final Research Report
  • [Presentation] A case of Enteropathy-associated T-cell lymphoma(EATL) complicating CLL/SLL2012

    • Author(s)
      Naoya Murata, Tadahiko Igarashi, Hiroyuki Irisawa, Kayoko Murayama, Yoshimasa Nakazato
    • Organizer
      第74回日本血液学会学術集会
    • Place of Presentation
      国立京都国際会館(京都)
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト膵臓がん細胞の浸潤・転移に対する経口可能なリゾホスファチジン酸受容体アンタゴニストKi16198の抑制作用2012

    • Author(s)
      佐藤幸市、小町麻由美、当房雅之、茂木千尋、岡島史和
    • Organizer
      第54回日本脂質生化学会
    • Place of Presentation
      九州大学医学部百年講堂(福岡)
    • Related Report
      2013 Final Research Report
  • [Presentation] Feasibility of MEAM therapy with autologous stem cell transplant for high-risk/relapsed lymphoma2011

    • Author(s)
      Kayoko Murayama, Tadahiko Igarashi, Naoya Murata, Hiroyuki Irisawa, Masaru Kojima
    • Organizer
      第73回日本血液学会学術集会
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Related Report
      2013 Final Research Report
  • [Presentation] Diffuse large B cell lymphoma(DLBCL) of stomach with fatal Edwardsiella tarda sepsis2011

    • Author(s)
      Naoya Murata, Hiroyuki Irisawa, Kayoko Murayama, Tadahiko Igarashi
    • Organizer
      第73回日本血液学会学術集会
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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