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Pseudogene affected gene expression via regulating microRNA in esopheal squamous cancer

Research Project

Project/Area Number 23591940
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Digestive surgery
Research InstitutionKumamoto University

Principal Investigator

NAGAI Yohei  熊本大学, 医学部附属病院, 非常勤診療医師 (30551254)

Co-Investigator(Kenkyū-buntansha) WATANABE Masayuki  公益財団法人がん研究会, がん研有明病院, 食道担当部長 (80254639)
HAYASHI Naoko  熊本大学, 医学部附属病院, 非常勤診療医師 (20452899)
BABA Yoshifumi  熊本大学, 大学院生命科学研究部, 講師 (20599708)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsmicroRNA / psuedogene / FBXW7 / pseudogene / ceRNAs / PTEN / 食道癌 / 偽遺伝子 / マイクロアレイ
Research Abstract

MicroRNAs (miRsare) ~22 nucleotide non-coding RNA molecules that regulate gene expression post-transcriptionally. Competitive endogenous RNAs (ceRNAs) sequester miRs to regulate mRNA transcripts containing common microRNA recognition elements. We investigated the relationship among the PTEN, PTENP1 by qRT-PCR in 40 cancer cell lines. In the 40 gastric cancer patients, we found a remarkably strong correlation between the expression revels of the PTEN and PTENP1. Next, we indicates that high expression of miR-223 had a significant adverse impact on the survival of esophageal squamous cell carcinoma patients through repression of the function of FBXW7. We sought that 3'UTR of FBXW7 will be therapy sponge which decoy of onco-miRs targeted FBXW7. However, up-regulated 3'UTR of FBXW7 did not affect the expression of miRs and FBXW7. Next, we suggests that miR-200b regulates the ZEB1/2 in gastric cancer and thus controls EMT in gastric cancer.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (28 results)

All 2013 2012 2011

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (18 results) Book (1 results)

  • [Journal Article] Clinical impact of serum exosomalmicroRNA-21 in human esophagealsquamous cell carcinoma, as a clinicalbiomarker2013

    • Author(s)
      Tanaka Y,Kammohara H,KinoshitaK,Kurashige J,Ishimoto T, Iwatsuki M,Watanabe M, Baba H
    • Journal Title

      Cancer

      Volume: 119 Issue: 6 Pages: 1159-1167

    • DOI

      10.1002/cncr.27895

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Overexpressionitin ligase FBXW7in esopha of microRNA-223 regulatesthe ubiqu geal squamous cellcarcinoma2012

    • Author(s)
      Kurashige J, Watanabe M, Iwatsuki M,Kinoshita K, Saito S,Hiyoshi Y,Kamohara H, Baba H, Mimori K
    • Journal Title

      Br J Cancer

      Volume: 106 Issue: 1 Pages: 182-188

    • DOI

      10.1038/bjc.2011.509

    • Related Report
      2013 Final Research Report 2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Micro RNA- 200b regulates cellproliferation, invasion and migrationby directly targeting ZEB2 in gastriccarcinoma2012

    • Author(s)
      Kurashige J, Kamohara H, Watanabe M,Hiyoshi Y, Iwatsuki M, Tanaka Y,Kinoshita K, Saito S, Baba Y, Baba H
    • Journal Title

      Ann Surg Oncol 19 Suppl

      Volume: 3 Issue: S3 Pages: 656-64

    • DOI

      10.1245/s10434-012-2217-6

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Serum microRNA-21 is a novel biomarker in patients with esophageal squamous cell carcinoma2012

    • Author(s)
      Kurashige J
    • Journal Title

      Journal of Surgical Oncology

      Volume: (印刷中) Issue: 2 Pages: 188-92

    • DOI

      10.1002/jso.23064

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Carcinogenesis of intraductal papillary mucinous neoplasm of the pancreas : loss of microrna-101 promotes overexpression of histone methyltransferase EZH22012

    • Author(s)
      Nakahara O
    • Journal Title

      Annals of Surgical Oncology

      Volume: (印刷中) Issue: S3 Pages: 565-71

    • DOI

      10.1245/s10434-011-2068-6

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Carcinogenesis of intraductal papillary mucinous neoplasm of the pancreas: Loss of MicroRNA-101 promotes overexpression of histone methyltransferase EZH2.2012

    • Author(s)
      Nakahara O, Takamori H, Iwatsuki M, Baba Y, Sakamoto Y, Tanaka H, Chikamoto A, Horino K, Beppu T, Kanemitsu K, Honda Y, Iyama K, Baba H
    • Journal Title

      Ann Surg Oncol

      Volume: 印刷中

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] MicroRNA-200b regulates cell proliferation, invasion and migration by directly targeting ZEB2 in gastric carcinoma.2012

    • Author(s)
      Kurashige J, Kamohara H, Watanabe M, Hiyoshi Y, Iwatsuki M, Tanaka Y, Kinoshita K, Saito S, Baba Y, Baba H
    • Journal Title

      Ann Surg Oncol

      Volume: 印刷中

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Serum MicroRNA-21 is a novel biomarker in patients with esophageal squamous cell carcinoma.)2012

    • Author(s)
      Kurashige J, Kamohara H, Watanabe M, Tanaka Y, Kinoshita K, Saito S, Hiyoshi Y, Iwatsuki M, Baba Y, Baba H
    • Journal Title

      J Surg Oncol

      Volume: 印刷中

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Clinical significance of Wnt-induced secreted protein-1 (WISP-1/CCN4) in esophageal squamous cell carcinoma2011

    • Author(s)
      Nagai Y, Watanabe M, Ishikawa S, Karashima R, Kurashige J, Iwagami S, Iwatsuki M, Baba Y, Imamura Y, Hayashi N, Baba H.
    • Journal Title

      Anticancer Res.

      Volume: 31(3) Pages: 991-7

    • URL

      http://ar.iiarjournals.org/content/31/3/991.full.pdf+html

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] miR-223は食道扁平上皮癌において癌抑制遺伝子ユビキチンリガーゼ複合体基質ユニットFBXW7の発現を制御する2012

    • Author(s)
      藏重淳二、渡邊雅之、岩槻政晃、馬場秀夫、森正樹、三森功士
    • Organizer
      第21回日本がん転移学会学術集会
    • Place of Presentation
      オリエンタルホテル広島
    • Related Report
      2013 Final Research Report
  • [Presentation] 食道扁平上皮癌においてmicroRNA-223は癌抑制遺伝子ユビキチンリガーゼ複合体基質ユニットFBXW7の発現を制御する2012

    • Author(s)
      藏重淳二、渡邊雅之、田中洋平、木下浩一、齋藤誠哉、岩槻政晃、馬場祥史、馬場秀夫
    • Organizer
      第112回日本外科学会定期学術集会
    • Place of Presentation
      幕張メッセ(千葉県)
    • Related Report
      2013 Final Research Report
  • [Presentation] Overexpression of microRNA-223 regulates the ubiquitin ligase FBXW7 in esophageal squamous cell carcinoma2012

    • Author(s)
      Kurashige J, Watanabe M, Iwatsuki M, Baba Y, Baba H
    • Organizer
      AACR Annual Meeting 2012
    • Place of Presentation
      アメリカ合衆国シカゴ
    • Related Report
      2013 Final Research Report
  • [Presentation] 胃癌におけるmicroRNA-200familyの発現と臨床病理学的検討2012

    • Author(s)
      藏重淳二、蒲原英伸、渡邊雅之、田中洋平、木下浩一、齋藤誠也、岩槻政晃、馬場祥史、馬場秀夫
    • Organizer
      第84回日本胃癌学会総会
    • Place of Presentation
      大阪国際会議場(グランキューブ大阪)
    • Related Report
      2013 Final Research Report
  • [Presentation] 食道扁平上皮癌においてmicroRNA-223は癌抑制遺伝子ユビキチンリガーゼ複合体基質ユニットFBXW7の発現を制御する2012

    • Author(s)
      蔵重淳二
    • Organizer
      第112回日本外科学会定期学術集会
    • Place of Presentation
      幕張メッセ 千葉県
    • Related Report
      2012 Research-status Report
  • [Presentation] Overexpression of microRNA-223 regulates the ubiquitin ligase FBXW7 in esophageal squamous cell carcinoma2012

    • Author(s)
      Kurashige J, Watanabe M, Iwatsuki M, Baba Y, Baba H
    • Organizer
      AACR Annual Meeting 2012
    • Place of Presentation
      McCormick Place West(アメリカ・シカゴ)
    • Related Report
      2012 Research-status Report
  • [Presentation] 胃癌におけるmicroRNA-200familyの発現と臨床病理学的検討2012

    • Author(s)
      蔵重淳二
    • Organizer
      第84回日本胃癌学会総会
    • Place of Presentation
      大阪 グランキューブ大阪
    • Related Report
      2011 Research-status Report
  • [Presentation] 胃癌におけるmicroRNA-200bによる浸潤転移機構の制御2011

    • Author(s)
      藏重淳二、渡邊雅之、田中洋平、木下浩一、齋藤誠也、日吉幸晴、岩槻政晃、馬場祥史、馬場秀夫
    • Organizer
      第20回日本がん転移学会学術集会
    • Place of Presentation
      アクトシティ浜松(静岡県)
    • Related Report
      2013 Final Research Report
  • [Presentation] MicroRNA-200b regulates Epithelial Mesenchymal Transition by targeting ZEB2 in Gastric Cancer2011

    • Author(s)
      Junji Kurashige , Masayuki Watanabe, Yukiharu Hiyoshi , Hidenobu Kamohara , Koichi Kinoshita, Seiya Saito, Masaaki Iwatsuki, Yoshifumi Baba , Naoko Hayashi , Hideo Baba
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Related Report
      2013 Final Research Report
  • [Presentation] Serum microRNA-21 is a novel biomarker in patients with esophageal squamous cell carcinoma2011

    • Author(s)
      Kurashige J
    • Organizer
      AACR102nd Annual Meeting 2011
    • Place of Presentation
      the Orange County Convention Center in Orlando, Florida
    • Related Report
      2011 Research-status Report
  • [Presentation] MicroRNA-29c expression as a new prognostic marker for esophageal squamous cell carcinoma2011

    • Author(s)
      Kinoshita K
    • Organizer
      AACR102nd Annual Meeting 2011
    • Place of Presentation
      the Orange County Convention Center in Orlando, Florida
    • Related Report
      2011 Research-status Report
  • [Presentation] Carcinogenesis of intraductal papillary mucinous neoplasm of the pancreas: Loss of microRNA-101 promotes overexpression of histone methyltransferase EZH22011

    • Author(s)
      Nakahara O
    • Organizer
      AACR102nd Annual Meeting 2011
    • Place of Presentation
      the Orange County Convention Center in Orlando, Florida
    • Related Report
      2011 Research-status Report
  • [Presentation] MIcroRNA-200B regulates epithelial to mesenchymal transition in gastric carcinoma2011

    • Author(s)
      Kurashige
    • Organizer
      9th International Gastric Cancer Congress
    • Place of Presentation
      Soeul Korea Coex Center
    • Related Report
      2011 Research-status Report
  • [Presentation] MicroRNA-200b regulates Epithelial Mesenchymal Transition by targeting ZEB2 in Gastric Cancer2011

    • Author(s)
      Kurahsige J
    • Organizer
      the 8th International Symposium on Minimal Residual Cancer
    • Place of Presentation
      大阪 グランキューブ大阪
    • Related Report
      2011 Research-status Report
  • [Presentation] The Identificationof novel micro RNAs and Genes in bone marrow regulating gastric cancer metastasis2011

    • Author(s)
      Iwatsuki M
    • Organizer
      the 8th International Symposium on Minimal Residual Cancer
    • Place of Presentation
      大阪 グランキューブ大阪
    • Related Report
      2011 Research-status Report
  • [Presentation] "リンパ節転移陽性食道癌に対する導入化学療法としてのmDCF療法の有用性と問題点 / The efficacy and issues of modified DCF therapy as induction chemotherapy for esophageal cancer with lymph node metastasis2011

    • Author(s)
      岩槻政晃
    • Organizer
      第111回日本外科学会定期学術集会
    • Place of Presentation
      紙上発表
    • Related Report
      2011 Research-status Report
  • [Presentation] 食道癌予後因子としてのmiR-29cの発現の意義 / MicroRNA-29c expression as a new prognostic marker as esophageal cancer2011

    • Author(s)
      木下浩一
    • Organizer
      第111回日本外科学会定期学術集会
    • Place of Presentation
      紙上発表
    • Related Report
      2011 Research-status Report
  • [Presentation] 食道癌患者血清から抽出したエキソソーム内のmicroRNAの発現解析 / Expression profiling of micro-RNAs in exosomes extracted from human esophageal cancer serum2011

    • Author(s)
      田中洋平
    • Organizer
      第111回日本外科学会定期学術集会
    • Place of Presentation
      紙上発表
    • Related Report
      2011 Research-status Report
  • [Book] 最新胃癌学基礎と臨床の最新研究動向「胃癌におけるマイクロRNA-200bの意義」2013

    • Author(s)
      藏重淳二、渡邊雅之、岩槻政晃、岩上志朗、馬場秀夫
    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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