Selective neural differentiation method using ES gut
Project/Area Number |
23591969
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Digestive surgery
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Research Institution | Nara Medical University |
Principal Investigator |
MISAWA Hiromi 奈良県立医科大学, 医学部, その他 (50281275)
|
Co-Investigator(Kenkyū-buntansha) |
TAKAKI Miyako 奈良県立医科大学, 医学部, 特任教授 (00033358)
MATSUYOSHI Hiroko 奈良県立医科大学, 医学部, 助教 (10448772)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 胚性幹細胞 / ES腸管 / セロトニン4受容体 / 腸壁内神経 / ハンギングドロップ / ペプチドゲル / 3次元培養 |
Research Abstract |
From mouse embryonic stem (ES) cells, the gut-like organ expressing peristalsis-like movements could be stably formed. In this gut, pacemaker cells (ICC) generating peristalsis-like movements could be stably differentiated without any differentiation inducing factors. In the present study, during the process forming the ES gut, a special neuron differentiation inducing factor (serotonin 4 receptor agonist) was applied, succeeding in formation of ES guts expressing more perfect peristalses. The results of this study would provide many hints to clarify the conditions and explore therapies of bowel diseases lacking enteric neurons.
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Report
(4 results)
Research Products
(12 results)
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[Presentation] ES gut-like organ2011
Author(s)
Takaki M, Misawa H, Matsuyoshi H, Kuniyasu H.
Organizer
An annual meeting of The Korean Society of Smooth Muscle Research in Seoul National University Bundang Hospital
Year and Date
2011-06-18
Related Report
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