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Selective neural differentiation method using ES gut

Research Project

Project/Area Number 23591969
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Digestive surgery
Research InstitutionNara Medical University

Principal Investigator

MISAWA Hiromi  奈良県立医科大学, 医学部, その他 (50281275)

Co-Investigator(Kenkyū-buntansha) TAKAKI Miyako  奈良県立医科大学, 医学部, 特任教授 (00033358)
MATSUYOSHI Hiroko  奈良県立医科大学, 医学部, 助教 (10448772)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords胚性幹細胞 / ES腸管 / セロトニン4受容体 / 腸壁内神経 / ハンギングドロップ / ペプチドゲル / 3次元培養
Research Abstract

From mouse embryonic stem (ES) cells, the gut-like organ expressing peristalsis-like movements could be stably formed. In this gut, pacemaker cells (ICC) generating peristalsis-like movements could be stably differentiated without any differentiation inducing factors.
In the present study, during the process forming the ES gut, a special neuron differentiation inducing factor (serotonin 4 receptor agonist) was applied, succeeding in formation of ES guts expressing more perfect peristalses.
The results of this study would provide many hints to clarify the conditions and explore therapies of bowel diseases lacking enteric neurons.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (12 results)

All 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (6 results) Remarks (1 results)

  • [Journal Article] In vivo imaging of enteric neurogenesis in the deep tissue of mouse smallintestine. hearts of SERCA2a transgenic rats.2013

    • Author(s)
      Goto K, Obata K, Takaki M, 他
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 1 Pages: e54814-e54814

    • DOI

      10.1371/journal.pone.0054814

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] In vitro morphological bud formation in organ-like three-dimensional structure from mouse ES cells induced by FGF10 signaling2012

    • Author(s)
      Ohbuchi, T. Takaki, M. Misawa, H. Suzuki, H. & Ueta, Y
    • Journal Title

      Communicative & Integrative Biology

      Volume: 5(4) Issue: 4 Pages: 1-4

    • DOI

      10.4161/cib.20093

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comparison of effects of a selective 5-HT reuptake inhibitor versus a 5-HT4 receptor agonist on in vivo neurogenesis at the rectal anasotomosis in rats.2012

    • Author(s)
      Kawahara I. Obata K, Takaki M
    • Journal Title

      Am J Physiol Gastrointest Liver Physiol

      Volume: 302 Issue: 6 Pages: 588-597

    • DOI

      10.1152/ajpgi.00284.2011

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] The comparison of effects of a selective 5-HT reuptake inhibitor versus a 5-HT4 receptor agonist on in vivo neurogenesis at the rectal anasotomosis in rats.2012

    • Author(s)
      Kawahara I, Kuniyasu H, Matsuyoshi H, Goto K, Obata K, Misawa H, Fujii H, Takaki M
    • Journal Title

      Am J Physiol Gastrointest Liver Physiol

      Volume: 302

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Invitro enhanced differentiation of neural networksin ES gut-like organ from mouse ES cells by a 5-HT4-receptor activation.2011

    • Author(s)
      Takaki M, Misawa H, Matsuyoshi H, Kawahara I,Goto K, Zhang GX, Obata K, Kuniyasu H
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 406 Issue: 4 Pages: 529-533

    • DOI

      10.1016/j.bbrc.2011.02.072

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 5-HT4受容体居性化を介したin vitro神経分化促進作用とin vivo神経再生・新生促進作用 (シンポジウム消化器疾患における5-HT受容体を標的にした新たな創薬展開)2012

    • Author(s)
      高木 都, 三澤 裕美, 松吉 ひろ子, 川原 勲, 後藤 桂, 小畑 孝二, 國安弘基
    • Organizer
      第85回日本薬理学会年会
    • Place of Presentation
      国立京都国際会館京都
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス小腸切離吻合術後の肉芽組織深部に新生した腸壁内神経系のin vivoイメージング法による解析2012

    • Author(s)
      後藤桂,加藤剛,川原勲,三澤裕美,石川達也,國安弘基,鍋倉淳一,高木 都
    • Organizer
      第40回自律神経生理研究会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] 5-HT4受容体活性化を介したin vitro神経分化促進作用とin vivo神経再生・新生促進作用2012

    • Author(s)
      高木 都
    • Organizer
      第85回日本薬理学会年会(シンポジウム)
    • Place of Presentation
      国立京都国際会館(京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] 選択的セロトニン再取り込み阻害薬fluvoxamine(SSRI)のin vitro神経分化,in vivo神経再生・新生作用に対する効果:セロトニン4受容体刺激薬との比較2011

    • Author(s)
      高木 都, 川原 勲, 三澤裕美, 後藤桂, 松吉ひろ子, 小畑孝二, 國安弘基
    • Organizer
      第13回日本神経消化器病学会
    • Place of Presentation
      栃木総合文化センター栃木
    • Year and Date
      2011-11-05
    • Related Report
      2013 Final Research Report
  • [Presentation] ES gut-like organ2011

    • Author(s)
      Takaki M, Misawa H, Matsuyoshi H, Kuniyasu H.
    • Organizer
      An annual meeting of The Korean Society of Smooth Muscle Research in Seoul National University Bundang Hospital
    • Year and Date
      2011-06-18
    • Related Report
      2013 Final Research Report
  • [Presentation] 選択的セロトニン再取り込み阻害薬 fluvoxamine (SSRI) のin vitro神経分化、in vivo神経再生・新生作用に対する効果:セロトニン4受容体刺激薬との比較2011

    • Author(s)
      高木 都
    • Organizer
      第13回日本神経消化器病学会
    • Place of Presentation
      栃木総合文化センター(栃木)
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.naramed-u.ac.jp/~2phy/Gyoseki.htm

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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