Discover the Full Breadth of Liver carcinogenesis and Treatment with Analysis of NPM

• Project/Area Number: 23591998
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: ENOMOTO Takeharu 聖マリアンナ医科大学, 医学部, 助教 (70350626)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: NPM / HBx / 国際情報交流

Research Abstract

HBx is well reported to be associated with Liver Carcinogenesis. We investigated that relation with multifunctional protein NPM and HBx. According to the Forgues et al. used HBxadr for examination so that we started to use same vector. But recently, Geng et al reported that they used new peptide sequences. We decided to use this new sequence. They had some mistakes in the sequences. We are figure out that problem and we made again from the beginning using Site-directed mutagenesis kit (TaKaRa). The effect of this step will be to delay having done experiments.