| Project/Area Number |
23592001
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
UYAMA Naoki 兵庫医科大学, 医学部, 講師 (70402873)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Jiro 兵庫医科大学, 医学部, 教授 (90199373)
IIMURO Yuji 兵庫医科大学, 医学部, 教授 (30252018)
HIRANO Tadamichi 兵庫医科大学, 医学部, 准教授 (90340968)
SATAKE Makoto 兵庫医科大学, 医学部, 助教 (70399153)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 肝内胆管癌 / Plakin / 癌細胞 / 線維芽細胞 / Ｐｌａｋｉｎ / Plakin Family / 癌関連繊維芽細胞 / 増殖能 / 転移能 / 肝内胆管がん / 癌関連線維芽細胞 |
|---|
| Outline of Final Research Achievements |
Plakin Family consists of cytoskeletal proteins such as plectin , desmoplakin and so on. In this project, we investigated expression of plectin and desmoplakin in human intrahepatic cholangiocarcinoma (ICC) and their fuctions. Immunohistochemistry revealed that these proteins were expressed by cancer cells as well as fibroblasts. In addition, integrin alpha6 and beta4, binding proteins of plectin, and desmocollin desmoglein, binding proteins of desmoplakin, were also expressed by cancer cells. Gene depletion experiments with SiRNA for these proteins revealed that they did not regulate ICC celllines function, such as proliferaion ability, migration ability and ability for activating fibroblas.
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