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Targeting therapy using chemotherapy-resistant pancreatic cancer stem cells

Research Project

Project/Area Number 23592007
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Digestive surgery
Research InstitutionKobe University

Principal Investigator

SHIMIZU Kazuya  神戸大学, 保健学研究科, 研究員 (50335353)

Co-Investigator(Kenkyū-buntansha) HORI Yuichi  神戸大学, 保健学研究科, 教授 (80248004)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords膵臓がん / がん幹細胞 / 癌幹細胞 / 膵臓癌 / 微小環境
Research Abstract

Pancreatic ductal adenocarcinoma is highly resistant to systemic chemotherapy. tabine-resistant pancreatic ductal adenocarcinoma patients. By use of in vitro co-culture system with stromal cells, we established a novel pancreatic tumor-initiating cell line. The cell line required its direct interaction with stromal cells for its in vitro clonogenic growth and passaging. Their direct interaction induced basal lamina-like extracellular matrix formation that maintained colony formation. The cell line expressed CD133 protein, which expression level changed autonomously and by culture conditions. These results demonstrated that there were novel pancreatic tumor-initiating cells that required direct interactions with stromal cells for their in vitro cultivation in gemcitabine-resistant pancreatic ductal adenocarcinoma. This cell line would help to develop novel therapies that enhance effects of gemcitabine or novel anti-cancer drugs.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (19 results)

All 2014 2013 2012

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (12 results) Book (2 results)

  • [Journal Article] Establishment of a novel cell line from intraductal papillary neoplasm of the bile duct.2014

    • Author(s)
      Murakami S, Ajiki T, Hori Y, Okazaki T, Fukumoto T, Ku Y.
    • Journal Title

      Anticancer Research

      Volume: 34 Pages: 2203-2209

    • NAID

      120005970749

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a novel subpopulation of tumor-initiating cells from gemcitabine-resistant pancreatic ductal adenocarcinoma patients.2013

    • Author(s)
      Shimizu K, Chiba S, Hori Y.
    • Journal Title

      PLoS One.

      Volume: 21 Issue: 11 Pages: e81283-e81283

    • DOI

      10.1371/journal.pone.0081283

    • NAID

      120005356910

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prominin-1 (CD133) Reveals New Faces of Pancreatic Progenitor Cells and Cancer Stem Cells: Current Knowledge and Therapeutic Perspectives.2013

    • Author(s)
      Hori Y.
    • Journal Title

      Adv Exp Med Biol.

      Volume: 777 Pages: 185-196

    • DOI

      10.1007/978-1-4614-5894-4_12

    • ISBN
      9781461458937, 9781461458944
    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Distinct expression of CD133 between intraductal papillary neoplasms of the bile duct and bile duct adenocarcinomas2012

    • Author(s)
      Ohtsubo I, Ajiki T, Hori Y, Murakami S, Shimizu K, Fukumoto T, Ku Y.
    • Journal Title

      Hepatol Res.

      Volume: Vol.42 Pages: 574-582

    • NAID

      10031144272

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Distinctive expression of CD133 between intraductal papillary neoplasms of the bile duct and bile duct adenocarcinomas.2012

    • Author(s)
      ohtsubo I
    • Journal Title

      Hepatology Research

      Volume: 42 Issue: 6 Pages: 574-582

    • DOI

      10.1111/j.1872-034x.2011.00954.x

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Gemcitabineが初代膵癌細胞に及ぼす効果の検討2013

    • Author(s)
      藤原範人、堀裕一
    • Organizer
      第45回日本臨床分子形態学会
    • Place of Presentation
      福岡
    • Year and Date
      2013-09-13
    • Related Report
      2013 Final Research Report
  • [Presentation] 膵組織幹細胞を用いた再生医療研究2013

    • Author(s)
      堀裕一、清水一也
    • Organizer
      第44回日本膵臓学会
    • Place of Presentation
      山形
    • Year and Date
      2013-07-25
    • Related Report
      2013 Final Research Report
  • [Presentation] 膵組織幹細胞を用いた再生医療研究2013

    • Author(s)
      堀裕一
    • Organizer
      第44回日本膵臓学会大会
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] 糖尿病の再生医療を目指したインスリン産生細胞の誘導2013

    • Author(s)
      神農祐樹、片山南詩、山崎千寛、平井里奈、藤原範人、三浦拓真、上野山恭平、西山嵩晃、堀裕一
    • Organizer
      第8回日本臨床検査学教育学会
    • Place of Presentation
      大阪
    • Related Report
      2013 Annual Research Report
  • [Presentation] Gemcitabineが初代膵癌細胞に及ぼす効果の検討 -新規治療薬の開発をめざして-2013

    • Author(s)
      藤原範人、清水一也、西山嵩晃、片山南詩、神農祐樹、平井里奈、三浦拓真、上野山恭平、山崎千寛、堀裕一
    • Organizer
      第45回日本臨床分子形態学会
    • Place of Presentation
      福岡
    • Related Report
      2013 Annual Research Report
  • [Presentation] 幹細胞マーカーラベルマウスを用いた膵再生医療研究2013

    • Author(s)
      堀裕一
    • Organizer
      JDDW
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] 膵癌の進展機構における組織幹細胞の関与2012

    • Author(s)
      堀裕一、清水一也
    • Organizer
      第43回日本膵臓学会
    • Place of Presentation
      山形
    • Year and Date
      2012-06-28
    • Related Report
      2013 Final Research Report
  • [Presentation] Direct interaction between human pancreatic cancer stem cells and stromal cells remodels extracellular matrix formation for cancer stem cell niche2012

    • Author(s)
      Shimizu K.
    • Organizer
      10^<th> International Society for Stem Cell Research
    • Place of Presentation
      横浜
    • Year and Date
      2012-06-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Direct interaction between pancreatic cancer stem cells and stromal cells remodels extracellular matrix formation for cancer stem cell niche2012

    • Author(s)
      Hori Y, Shimizu K.
    • Organizer
      103^<rd> American Association for Cancer Research
    • Place of Presentation
      Chicago, USA
    • Year and Date
      2012-04-01
    • Related Report
      2013 Final Research Report
  • [Presentation] Direct interaction between human pancreatic cancer stem cells and stromal cells remodels extracellular matrix formation for cancer stem cell niche.2012

    • Author(s)
      Shimizu K
    • Organizer
      10th International Society for Stem Cell Research
    • Place of Presentation
      Yokohama
    • Related Report
      2012 Research-status Report
  • [Presentation] Direct interaction between human pancreatic cancer stem cells and stromal cells remodels extracellular matrix formation for cancer stem cell niche.2012

    • Author(s)
      Hori Y
    • Organizer
      103rd American Association for Cancer Research
    • Place of Presentation
      Chicago, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Direct interaction between human pancreatic cancer stem cells and stromal cells remodels extracellular matrix formation for cancer stem cell niche.2012

    • Author(s)
      堀裕一、清水一也、中村幸恵
    • Organizer
      American Association for Cancer Research
    • Place of Presentation
      Chicago
    • Related Report
      2011 Research-status Report
  • [Book] 肝胆膵2013

    • Author(s)
      堀裕一
    • Total Pages
      734
    • Publisher
      アークメディア
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Book] 肝胆膵2013

    • Author(s)
      堀裕一
    • Publisher
      アークメディアクメディア
    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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