Low-energy extracorporeal shock wave therapy promotes VEGF expression and neuroprotection and improves locomotor recovery after spinal cord injury

• Project/Area Number: 23592153
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Tohoku University
• Principal Investigator: OZAWA Hiroshi 東北大学, 医学(系)研究科(研究院), 准教授 (10312563)
• Co-Investigator(Kenkyū-buntansha): ITO Kenta 東北大学, 大学院医学系研究科, 准教授 (50375086)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 脊髄損傷 / 体外衝撃波 / VEDF / 低出力体外衝撃波治療

Research Abstract

The present study investigated whether the low-energy ESWT can increase VEGF expression to provide neuroprotective effect and enhance the locomotor recovery after SCI in rats. Adult female Sprague-Dawley rats were divided into four groups: Sham group (laminectomy only), Sham-SW group (ESWT applied after laminectomy), SCI group and SCI-SW group (ESWT applied after SCI). The low-energy ESWT was applied to the injured spinal cord 3 times a week for 3 weeks after SCI. The low-energy ESWT significantly reduced neuronal loss in damaged neural tissue. Furthermore, locomotor function was significantly improved in animals in SCI-SW group compared to SCI group after SCI. These results demonstrated that the low-energy ESWT enhanced neuroprotective effect of VEGF and improve locomotor recovery following SCI.

Research Products

• [Journal Article] Combination of engineered Schwann cell grafts to secrete neurotrophin and chondroitinase promotes axonal regeneration and locomotion after spinal cord injury (2014)
  • Author(s): Kanno H, Pressman Y, Moody A, Berg R, Muir EM, Rogers JH, Ozawa H, Itoi E, Pearse DD, Bunge MB
  • Journal Title: J Neuroscience Volume: 29,34(5) Pages: 1838-55
  • Peer Reviewed
• [Journal Article] Combination of engineered Schwann cell grafts to secrete neurotrophin and chondroitinase promotes axonal regeneration and locomotion after spinal cord injury. (2014)
  • Author(s): Kanno H, Pressman Y, Moody A, Berg R, Muir EM, Rogers JH, Ozawa H, Itoi E, Pearse DD, Bunge MB.
  • Journal Title: J Neurosci Volume: Jan 29;34(5) Pages: 1838-55
  • Peer Reviewed
• [Journal Article] Influence of intramedullary stress on cervical spondylotic myelopathy (2013)
  • Author(s): Takahashi K, Ozawa H, Sakamoto N, Minegishi Y, Sato M, Itoi E
  • Journal Title: Spinal Cord Volume: 51(10) Pages: 761-4
  • Peer Reviewed
• [Journal Article] Atypical findings on magnetic resonance imaging in the patients with active pyogenic spondylitis in Japanese university hospitals. (2013)
  • Author(s): Aizawa T, Ozawa H, Koakutsu T, Nakamura T, Kanno H, Hirano T, Sano A, Ito T, Itoi E.
  • Journal Title: Tohoku J Exp Med. Volume: 231 Pages: 13-19
  • NAID: 10031201050
  • Peer Reviewed
• [Journal Article] Influence of intramedullary stress on cervical spondylotic myelopathy. (2013)
  • Author(s): Takahashi K, Ozawa H, Sakamoto N, Minegishi Y, Sato M, Itoi E.
  • Journal Title: Spinal Cord. Volume: Oct;51(10) Pages: 761-4
• [Journal Article] Epidemiology of Surgically Treated Primary Spinal Cord Tumors in Miyagi, Japan. (2013)
  • Author(s): Ozawa H, Aizawa T, Kanno H, Sano H, Itoi E.
  • Journal Title: Neuroepidemiology. Volume: 41 Pages: 156-160
• [Journal Article] signaling pathway in spinal cord injury (2012)
  • Author(s): Kanno H, Ozawa H, Sekiguchi A, Yamaya S, Tateda S, Yahata K, Itoi E. The role of mTOR
  • Journal Title: Cell Cycle Volume: 11 Pages: 3175-9
  • Peer Reviewed
• [Journal Article] Rapamycin promotes autophagy and reduces neural tissue damage and locomotor impairment after spinal cord injury in mice (2012)
  • Author(s): Sekiguchi A, Kanno H, Ozawa H, Yamaya S, Itoi E
  • Journal Title: J Neurotrauma Volume: 29 Pages: 946-56
  • Peer Reviewed
• [Journal Article] Extracorporeal shock wave therapy improves the walking ability of patients with peripheral artery disease and intermittent claudication (2012)
  • Author(s): Serizawa F, Ito K, Kawamura K, Tsuchida K, Hamada Y, Zukeran T, Shimizu T, Akamatsu D, Hashimoto M, Goto H, M Watanabe T, Sato A, Shimokawa H, Satomi S
  • Journal Title: Circ J Volume: 76 Pages: 1486-93
  • NAID: 10030316519
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/22447002
  • Peer Reviewed
• [Journal Article] Low-energy extracorporeal shock wave therapy enhances skin wound healing in diabetic mice : a critical role of endothelial nitric oxide synthase (2012)
  • Author(s): Hayashi D, Kawakami K, Ito K, Ishii K, Tanno H, Imai Y, Kanno E, Maruyama R, Shimokawa H, Tachi M
  • Journal Title: Wound Repair Regen Volume: 20 Pages: 887-95
  • NAID: 10031181879
  • Peer Reviewed
• [Journal Article] Induction of autophagy and autophagic cell death in damaged neural tissue after acute spinal cord injury in mice (2011)
  • Author(s): Kanno H, Ozawa H, Sekiguchi A, Yamaya, S, Itoi E
  • Journal Title: Spine Volume: 36
  • Peer Reviewed
• [Journal Article] Extracorporeal shock wave therapy for ischemic cardiovascular disorders (2011)
  • Author(s): Ito K, Fukumoto Y, Shimokawa H
  • Journal Title: Am J Cardiovasc Drugs Volume: 11(5) Pages: 295-302
  • Peer Reviewed
• [Journal Article] Low-energy extracorporeal shock wave therapy promotes VEGF expression and neuroprotection and improves locomotor recovery after spinal cord injury
  • Author(s): Yamaya S, Ozawa H, Kanno H, Kishimoto KN, Sekiguchi A, Tateda S, Yahata K, Ito K, Shimokawa H, Itoi E
  • Journal Title: Journal of Neurosurgery. J Neurosurg Volume: (in press)
  • Peer Reviewed
• [Presentation] Therapeutic Potential of Low- Energy Extracorporeal Shock Wave for Neuropathic Pain after Spinal Cord Injury (2014)
  • Author(s): Tateda S, Ozawa H, Kanno H, Yamaya S, Yahata K, Ito K, Shimokawa H, Itoi E
  • Organizer: 60th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: New Orleans United States
• [Presentation] Rapamycin Suppresses Astrocytic and Microglial Activation and Reduces Development of Neuropathic Pain after Spinal Cord Injury in Mice (2014)
  • Author(s): Tateda S, Kanno H, Ozawa H, Yahata K, Yamaya S, Itoi E
  • Organizer: 60th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: New Orleans United States
• [Presentation] Upregulation of RIP3 Expression in Various Neural Cells after Spinal Cord Injury in Mice (2014)
  • Author(s): Kanno H, Ozawa H, Tateda S, Yahata K, Itoi E
  • Organizer: 60th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: New Orleans United States
• [Presentation] Low- energy Extracorporeal Shock Wave Therapy Promotes VEGF Expression in Various Neural Cells and Enhances Neuroprotective Effect after Spinal Cord Injury (2014)
  • Author(s): Yahata K, Ozawa H, Kanno H, Yamaya S, Tateda S, Ito K, Shimokawa H, Itoi E
  • Organizer: 60th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: New Orleans United States
• [Presentation] Postoperative Spinal Cord Swelling and Intramedullary Gd- DTPA Enhancement in Cervical Spondylotic Myelopathy (2014)
  • Author(s): Ozawa H, Sato T, Aizawa T, Itoi E, Kokubun S
  • Organizer: 60th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: New Orleans United States
• [Presentation] Therapeutic Potential of Low-Energy Extracorporeal Shock Wave for Neuropathic Pain after Spinal Cord Injury (2014)
  • Author(s): Tateda S, Ozawa H, Kanno H, Yamaya S, Yahata K, Ito K, Shimokawa H, Itoi E.
  • Organizer: 60th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: New Orleans (United States)
• [Presentation] Rapamycin Suppresses Astrocytic and Microglial Activation and Reduces Development of Neuropathic Pain after Spinal Cord Injury in Mice. (2014)
  • Author(s): Tateda S, Kanno H, Ozawa H, Yahata K, Yamaya S, Itoi E.
  • Organizer: 60th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: New Orleans (United States)
• [Presentation] Upregulation of RIP3 Expression in Various Neural Cells after Spinal Cord Injury in Mice (2014)
  • Author(s): Kanno H, Ozawa H, Tateda S, Yahata K, Itoi E.
  • Organizer: 60th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: New Orleans (United States)
• [Presentation] Low-energy Extracorporeal Shock Wave Therapy Promotes VEGF Expression in Various Neural Cells and Enhances Neuroprotective Effect after Spinal Cord Injury (2014)
  • Author(s): Yahata K, Ozawa H, Kanno H, Yamaya S, Tateda S, Ito K, Shimokawa H, Itoi E.
  • Organizer: 60th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: New Orleans (United States)
• [Presentation] Postoperative Spinal Cord Swelling and Intramedullary Gd-DTPA Enhancement in Cervical Spondylotic Myelopathy (2014)
  • Author(s): Ozawa H, Sato T, Aizawa T, Itoi E, Kokubun S.
  • Organizer: 60th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: New Orleans (United States)
• [Presentation] シュワン細胞移植による脊髄損傷治療–コンドロイチナーゼ遺伝子導入によるグリア瘢痕形成の抑制と神経軸索再生の促進– (2013)
  • Author(s): 菅野晴夫, Damien Pearse, Mary Bunge,小澤浩司,井樋栄二.
  • Organizer: 第28回日本整形外科学会基礎学術集会
  • Place of Presentation: 千葉
• [Presentation] 脊髄損傷に対する低出力体外衝撃波治療-アロディニアへの効果の検討- (2013)
  • Author(s): 舘田聡,菅野晴夫,小澤浩司,山屋誠司,八幡健一郎,伊藤健太,下川宏明,井樋栄二
  • Organizer: 第28回日本整形外科学会基礎学術集会
  • Place of Presentation: 千葉
• [Presentation] 急性期脊髄損傷における低出力体外衝撃波治療のVEGF発現促進・運動機能改善効果 (2013)
  • Author(s): 八幡健一郎,菅野晴夫,小澤浩司,山屋誠司,舘田聡,伊藤健太,下川宏明,井樋栄二
  • Organizer: 第28回日本整形外科学会基礎学術集会
  • Place of Presentation: 千葉
• [Presentation] Low-energy Extracorporeal Shock Wave Therapy Improved Motor Palsy after Spinal Cord Injury (2013)
  • Author(s): Yamaya S, Ozawa H, Kanno H, Sekiguchi A, Itoi E
  • Organizer: 2013 Annual Meeting, American Academy of Orthopaedic Surgeons
  • Place of Presentation: Chicago, United States
• [Presentation] Combination of engineered Schwann cell implants to secrete neurotrophin and chondroitinase promotes axonal regeneration and locomotion after spinal cord injury (2013)
  • Author(s): Kanno H, Pressman Y, Moody A, Berg R, Muir EM, Rogers JH, Ozawa H, Itoi E, Pearse DD, Bunge MB
  • Organizer: 59th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: San Antonio, United States
• [Presentation] Spinal Cord Swelling after Decompression Surgery in Cervical Myelopathy (2013)
  • Author(s): Ozawa H, Sato T, Aizawa T, Kanno H, Itoi E, Kokubun S.
  • Organizer: 9th Asia Pacific Spine Society Congress
  • Place of Presentation: Kuching (Malaysia)
• [Presentation] Increased facet fluid predicts dynamic change in the dural sac size on axial loaded MRI in patients with lumbar spinal canal stenosis (2013)
  • Author(s): Kanno H, Ozawa H, Koizumi Y, Morozumi N, Aizawa T, Hashimoto K, Ishii Y, Itoi E
  • Organizer: Tohoku-Ramathibodi Joint Conference 2013
  • Place of Presentation: Bangkok Thailand
• [Presentation] 荷重MRIによる腰椎変性すべり症の評価 (2013)
  • Author(s): 菅野晴夫, 小澤浩司, 古泉豊, 両角直樹, 石井祐信, 井樋栄二
  • Organizer: 第86回日本整形外科学会学術集会
  • Place of Presentation: 広島
• [Presentation] シュワン細胞移植による脊髄損傷治療ーコンドロイチナーゼ遺伝子導入によるグリア瘢痕形成の抑制と神経軸索再生の促進ー (2013)
  • Author(s): 菅野晴夫, Damien Pearse, Mary Bunge, 小澤浩司, 井樋栄二
  • Organizer: 第28回日本整形外科学会基礎学術集会
  • Place of Presentation: 千葉
• [Presentation] 脊髄損傷に対する低出力体外衝撃波治療ーアロディニアへの効果の検討ー (2013)
  • Author(s): 舘田聡, 菅野晴夫, 小澤浩司, 山屋誠司, 八幡健一郎, 伊藤健太, 下川宏明, 井樋栄二
  • Organizer: 第28回日本整形外科学会基礎学術集会
  • Place of Presentation: 千葉
• [Presentation] Low-Energy Extracorporeal Shock Wave Therapy Improves Motor Palsy after Spinal Cord Injury (2013)
  • Author(s): Seiji Yamaya, Hiroshi Ozawa
  • Organizer: AAOS（北米整形外科学会） 2013
  • Place of Presentation: Chicago, USA
• [Presentation] 脊髄損傷に対する低出力体外衝撃波の効果とメカニズムの検討 (2012)
  • Author(s): 山屋誠司,菅野晴夫,小澤浩司,八幡健一郎,関口玲,岸本光司,伊藤健太,下川宏明,井樋栄二
  • Organizer: 第27回日本整形外科学会基礎学術集会
  • Place of Presentation: 名古屋
• [Presentation] 神経栄養因子発現遺伝子およびコンドロイチナーゼ遺伝子を導入したシュワン細胞移植による脊髄損傷治療の検討 (2012)
  • Author(s): 菅野晴夫, Damien Pearse, Mary Bunge,小澤浩司,井樋栄二
  • Organizer: 第27回日本整形外科学会基礎学術集会
  • Place of Presentation: 名古屋
• [Presentation] Effect of low-energy extracorporeal spinal shock wave therapy on spinal cord injury (2012)
  • Author(s): Yamaya S, Ozawa H, Kanno H, Sekiguchi A, Kishimoto KN, Itoi E
  • Organizer: 58th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: San Francisco, United States
• [Presentation] The relationship between temperature and lubricin expression (2012)
  • Author(s): Sekiguchi A, Kishimoto KN, Hatta T, Okuno H, Oyanagi T, Ozawa H, Itoi E
  • Organizer: 58th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: San Francisco, United States.
• [Presentation] 脊髄損傷に対する低出力体外衝撃波の効果とメカニズムの検討 (2012)
  • Author(s): 山屋 誠司 小澤 浩司
  • Organizer: 第２７回 日本整形外科学会基礎学術集会
  • Place of Presentation: 名古屋
• [Presentation] Effect of Low-Energy Extracorporeal Shock Wave (2012)
  • Author(s): YAMAYA S, OZAWA H, ITO K, SHIMOKAWA H, ITOI E
  • Organizer: 2012 Orthopaedic Research Society Annual Meeting
  • Place of Presentation: San Francisco, CA, USA
• [Presentation] 脊髄損傷に対する低出力体外衝撃波の効果 (2011)
  • Author(s): 山屋誠司,小澤浩司,関口玲,菅野晴夫,岸本光司,井樋栄二
  • Organizer: 第26回日本整形外科学会基礎学術集会
  • Place of Presentation: 前橋
• [Presentation] 脊髄損傷におけるラパマイシンのautophagy促進効果と神経保護作用 (2011)
  • Author(s): 関口玲,菅野晴夫,小澤浩司,山屋誠司,井樋栄二
  • Organizer: 第26回日本整形外科学会基礎学術集会
  • Place of Presentation: 前橋
• [Presentation] Autophagy inducer rapamycin S reduces neural tissue damage and locomotor impairment after spinal cord injury in mice (2011)
  • Author(s): Sekiguchi A, Kanno H, Ozawa H, Yamaya S, Itoi E
  • Organizer: 57th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: Long Beach, United States
• [Presentation] 脊髄損傷に対する低出力体外衝撃波の効果 (2011)
  • Author(s): 山屋誠司、小澤浩司、関口玲、菅野晴夫、岸本光司、井樋栄二
  • Organizer: 第26回日本整形外科学会基礎学術集会
  • Place of Presentation: 日本、高崎
• [Book] Neuroprotection and Regeneration of the Spinal Cord (2014)
  • Author(s): Uchida K, Nakamura M, Ozawa H, Katoh S
  • Total Pages: 322
  • Publisher: Springer
• [Book] Neuroprotection and Regeneration of the Spinal Cord (2014)
  • Author(s): Uchida K, Nakamura M, Ozawa H, Katoh S.
  • Total Pages: 419
  • Publisher: Springer