Intracellular signaling of neuroprotection by general anesthetics

• Project/Area Number: 23592248
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Gifu University
• Principal Investigator: TANABE Kumiko 岐阜大学, 医学部附属病院, 講師 (30402209)
• Co-Investigator(Kenkyū-buntansha): KOZAWA Osamu 岐阜大学, 医学系研究科, 教授 (90225417)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 全身麻酔薬 / 中枢神経保護 / サイトカイン / アストロサイト / デクスメデトミジン / FGF-2 / GDNF / PI3-kinase/Akt経路 / ベンゾジアゼピン

Research Abstract

General anesthetics are reportedly to have neuroprotective effects. However, the detailed intracellular mechanisms have not been fully clarified. We investigated effects of intravenous anesthetics on neurotrophic factor or cytokine release from rat glioma cell line, C6 cells. In conclusion, midazolam or dexmedetomidine suppressed IL-1beta-induced IL-6 release. Midazolam inhibited IL-1beta-induced IL-6 via suppression of STAT3 phosphorylation in C6 glioma cells. However, exact mechanism of dexmedetomidine in IL-1beta-induced IL-6 release was not elucidated.

Research Products

• [Journal Article] Different effects of local anesthetics on extracellular signal-regulated kinase phosphorylation in rat dorsal horn neurons (2014)
  • Author(s): Zhang L,Tanabe K, Yanagidate F, Kawasaki Y, Chen G, Dohi S, Iida H
  • Journal Title: Eur J Parmacology Volume: (In Press)
  • Peer Reviewed
• [Journal Article] Dexmedetomidine suppresses interleukin-1β-induced interleukin-6 synthesis in rat glial cells (2014)
  • Author(s): Tanabe K, Matsushima-Nishiwaki R, Kozawa O, Iida H
  • Journal Title: Int J Mol Med Volume: (In Press)
  • Peer Reviewed
• [Journal Article] Dexmedetomidine suppresses interleukin-1β-induced interleukin-6 synthesis in rat glial cells. (2014)
  • Author(s): Tanabe K, Matsushima-Nishiwaki R, Kozawa O, Iida H
  • Journal Title: International Journal of Molecular Medicine Volume: In Press
  • Peer Reviewed
• [Journal Article] Different effects of local anesthetics on extracellular signal-regulated kinase phosphorylation in rat dorsal horn neurons. (2014)
  • Author(s): Zhang L, Tanabe K, Yanagidate F, Kawasaki Y, Chen G, Dohi S, Iida H
  • Journal Title: European Journal of Pharmacology Volume: In Press Pages: 132-136
  • DOI: 10.1016/j.ejphar.2014.03.048
  • Peer Reviewed
• [Journal Article] Nicorandil protects pial arterioles from endothelial dysfunction induced by smoking in rat. (2013)
  • Author(s): Iwata K, Iida H, Iida M, Takenaka M, Tanabe K, Fukuoka N, Uchida M
  • Journal Title: Journal of Neurosurgical Anesthesiology Volume: 25 Issue: 4 Pages: 392-398
  • DOI: 10.1097/ana.0b013e318295aa93
  • Peer Reviewed
• [Journal Article] Nicorandil protects pial arterioles from endothelial dysfunction induced by smoking in rats. (2013)
  • Author(s): Iwata K
  • Journal Title: J Neurosurg Anesth Volume: in press Issue: 6 Pages: 928-931
  • DOI: 10.1007/s00540-012-1433-3
  • Peer Reviewed
• [Journal Article] Intrathecal endothelin-1 has antinociceptive effects in rat model of postoperative pain (2012)
  • Author(s): Chen G, Tanabe K, Yanagidate F, Kawasaki Y, Zhang L, Dohi S, Iida H
  • Journal Title: Eur. J. Pharmacol. Volume: 696 Pages: 40-46
  • Peer Reviewed
• [Journal Article] Involvement of phosphatidylinositol 3-kinase/Akt on basic fibroblast growth factor-induced glial cell line-derived neurotrophic factor release from rat glioma cells (2012)
  • Author(s): Tanabe K, Matsushima-Nishiwaki R, Iida M, Kozawa O, Iida H
  • Journal Title: Brain Res Volume: 1463 Pages: 21-29
  • Peer Reviewed
• [Journal Article] Involvement of phosphatidylinosiol 3-kinase/Akt on basic fibroblast growth factor-induced glial cell line-derived neurotrophic factor release from rat glioma cells. (2012)
  • Author(s): Tanabe K
  • Journal Title: Brain Reseach. Volume: 1463 Pages: 21-29
  • DOI: 10.1016/j.brainres.2012.04.057
  • Peer Reviewed
• [Journal Article] Intrathecal endothelin-1 has antinociceptive effects in rat model of postoperative pain. (2012)
  • Author(s): Chen K
  • Journal Title: Eur J Pharmacology. Volume: 697 Issue: 1-3 Pages: 40-46
  • DOI: 10.1016/j.ejphar.2012.09.035
  • Peer Reviewed
• [Journal Article] Anesthetic management for a patient with very-long-chain acyl-coenzyme A dehydrogenase deficiency. (2012)
  • Author(s): Iwata K
  • Journal Title: J Anesth. Volume: 26 Issue: 6 Pages: 957-958
  • DOI: 10.1007/s00540-012-1457-8
  • Peer Reviewed
• [Journal Article] うつ病患者に対する修正型電気痙攣療法による脳血流の変化 (2012)
  • Author(s): 上松友希、中村好美、天野雄平、田辺久美子、飯田宏樹
  • Journal Title: 麻酔 Volume: 61 Pages: 126-129
  • Peer Reviewed
• [Journal Article] Midazolam suppresses interleukin-1β-induced interleukin-6 release from rat glial cells (2011)
  • Author(s): Tanabe K, Kozawa O, Iida H
  • Journal Title: J Neuroinflammation Volume: 8 Pages: 68-68
  • NAID: 120006341957
  • Peer Reviewed
• [Journal Article] Midazolam suppresses interleukin-1β-induced interleukin-6 release from rat glial cells (2011)
  • Author(s): Tanabe K, Kozawa O, Iida H
  • Journal Title: Journal of Neuroinflammmation Volume: 8 Issue: 1 Pages: 68-68
  • DOI: 10.1186/1742-2094-8-68
  • NAID: 120006341957
  • Peer Reviewed
• [Presentation] Midazolamのinterleukin-1βによるグリア細胞からのinterleukin-6遊離抑制機序 (2013)
  • Author(s): 田辺久美子
  • Organizer: 日本麻酔科学会第60回学術集会
  • Place of Presentation: 札幌
• [Presentation] Rho-kinase阻害薬によるグリア細胞からのInterleukin-6産生抑制機序 (2011)
  • Author(s): 田辺久美子
  • Organizer: 日本麻酔科学会第58回学術集会
  • Place of Presentation: 神戸
• [Presentation] Rho-kinase阻害薬によるグリア細胞からのInterleukin-6産生抑制機序 (2011)
  • Author(s): 田辺久美子、山口忍、飯田宏樹
  • Organizer: 58回日本麻酔学会総会
  • Place of Presentation: 神戸