| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
We have showed that xeroderma pigmentosum group A (Xpa) gene-knockout mice [Xpa (-/-) mice] provide a valuable model for impaired spermatogenesis as well as for UV-induced skin tumors in XPA patients. Gene expression analysis of each aged Xpa mice testis showed a different expression pattern of DNA repair gene, cell cycle-related gene(checkpoint), apoptosis-related gene compared to control. We tried to check the different expression pattern of these genes by Real-time PCR and immunohistochemistry. We did not detected these difference of these genes by the above methods. However, we found other pathology of vacuole formation in the aged Xpa mice testis. These findings will help us to understand the impaired spermatogenesis in the Xpa mice.
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