Human chorionic gonadotropin mediated intercellular signalling in human endometrial epithelial cells at implantation site

• Project/Area Number: 23592399
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: TAMURA Naoaki 浜松医科大学, 医学部附属病院, 助教 (90402370)
• Co-Investigator(Kenkyū-buntansha): KANAYAMA Naohiro 浜松医科大学, 医学部, 教授 (70204550) ; SUGIHARA Kazuhiro 浜松医科大学, 医学部, 准教授 (00265878)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 着床 / ヒト絨毛性ゴナドトロピン / 子宮内膜上皮細胞 / トロフィニン / 子宮内膜 / トロフォブラスト / hCG / MUC1 / 胎盤 / 子宮

Research Abstract

Under hCG treatment, expression levels of trophinin was enhanced and MUC1 was decreased in Ishikawa cells, one of endometrial epithelial cell lines. These alternations were more remarkable under hCG and IL-1beta treatment. From the Microarray analysis, gene expression levels of ADM and KISS1R were decreased in Ishikawa cells under hCG treatment.Genetic splicing variants of trophinin existed in Ishikawa cells. This fact suggested that trophinin may coordinate its function by dominant negative manner in vivo.

Research Products

• [Journal Article] Association between body weight at weaning and remodeling in the subcutaneous adipose tissue of obese adult mice with undernourishment in utero (2013)
  • Author(s): Y.K. Kohmura, N. Kanayama, K. Muramatsu, N. Tamura, C. Yaguchi, T. Uchida, K. Suzuki, K. Sugihara, S. Aoe, T. Sasaki, T. Suganami, Y. Ogawa, H. Itoh
  • Journal Title: Reprod. Sci. Volume: 20 Issue: 7 Pages: 813-827
  • DOI: 10.1177/1933719112466300
  • Peer Reviewed
• [Journal Article] Fatal amniotic fluid embolism with typical pathohistological, histochemical and clinical features (2013)
  • Author(s): Hikiji W, Tamura N, Shigeta A, Kanayama N, Fukunaga T
  • Journal Title: Forensic Sci Int Volume: 226 Issue: 1-3 Pages: 16-19
  • DOI: 10.1016/j.forsciint.2012.12.008
  • Peer Reviewed
• [Journal Article] Zinc coproporphyrin I derived from meconium has an antitumor effect associated with singlet oxygen generation (2013)
  • Author(s): Ozawa H, Asahina T, Murakami H, Yaguchi C, Kohmura Y, Kohno E, Hirano T, Horiuchi K, Tamura N, Sugihara K, Ooi H, Kanayama N
  • Journal Title: Fetal Diagn Ther Volume: 33 Issue: 2 Pages: 90-97
  • DOI: 10.1159/000342419
  • Peer Reviewed
• [Journal Article] Trophinin in cell adhesion and signal transduction (2012)
  • Author(s): Fukuda MN, Sugihara K
  • Journal Title: Front Biosci Volume: 1(4) Pages: 342-50
  • Peer Reviewed
• [Journal Article] Enhancement of mouse sperm tail motility by trophinin-binding peptide (2012)
  • Author(s): Park SK, Yoon J, Wang L, Shibata TK, Motamedchaboki K, Shim KJ, Chang MS, Lee SH, Tamura N, Hatakeyama S, Nadano D, Sugihara K, Fukuda MN
  • Journal Title: Reprod Biol Endocrin Volume: 10 Issue: 1 Pages: 101-101
  • DOI: 10.1186/1477-7827-10-101
• [Journal Article] Cerebral oxygen saturation evaluated by near-infrared time-resolved spectroscopy (TRS) in pregnant women during caesarean section - a promising new method of maternal monitoring (2012)
  • Author(s): Kaori Yamazaki
  • Journal Title: Clin Physiol Funct Imaging Volume: 32 Issue: 2 Pages: 109-116
  • DOI: 10.1111/cpf.12001
  • Peer Reviewed
• [Journal Article] CLP36 and RIL recruit α -actinin-1 to stress fibers and differentially regulate stress fiber dynamics in F2408 fibroblasts (2012)
  • Author(s): Miyazaki K, Ohno K, Tamura N, Sasaki T, Sato K
  • Journal Title: Exp Cell Res Volume: 318 Issue: 14 Pages: 1716-25
  • DOI: 10.1016/j.yexcr.2012.05.006
  • Peer Reviewed
• [Journal Article] Reduction in Levels of Maternal Complement during Delivery by Cesarean Section (2012)
  • Author(s): Kato M, Itoh H, Nagahashi K, Izima M, Hirai K, Tamura M, Uchida T, Yaguchi C, Suzuki K, Sugihara K, Kanayama K.
  • Journal Title: Journal of Obstetrics and Gynaecology Research Volume: 38 Issue: 1 Pages: 165-171
  • DOI: 10.1111/j.1447-0756.2011.01661.x
  • Peer Reviewed
• [Journal Article] Immunohistochemical detection of meconium in the fetal membrane, placenta and umbilical cord (2012)
  • Author(s): Naomi Fruta
  • Journal Title: Placenta Volume: 33 Issue: 1 Pages: 24-30
  • DOI: 10.1016/j.placenta.2011.10.007
  • Peer Reviewed
• [Journal Article] Changes in cervical neutrophil elastase levels during the menstrual cycle. (2012)
  • Author(s): Tawara F, Tamura N, Suganuma N, Kanayama N.
  • Journal Title: Reprod Med Biol Volume: 11 Pages: 65-68
  • NAID: 10030445109
  • Peer Reviewed
• [Journal Article] Feto-Umbilical blood flow obstruction increases placental tissue oxygenation. (2012)
  • Author(s): Suzuki K, Itoh H, Myramatsu K, Yamazaki K, Furuta N, Nagahashi K, Tamura N, Kato M, Uchida U, Sugihara K, Sumimoto K, Kanayama N.
  • Journal Title: Clin Exp Obstet Gynecol Volume: In press
  • Peer Reviewed
• [Journal Article] DIC初発羊水塞栓症と心肺虚脱羊水塞栓症の血清マーカーの比較（羊水塞栓症登録事業） (2012)
  • Author(s): 木村聡，田村直顕，内田季之，鈴木一有，森島賀子，伊東宏晃，金山尚裕.
  • Journal Title: 日本産婦人科・新生児血液学会誌 Volume: 21 Pages: 55-59
• [Journal Article] Targeted drug delivery to tumor vasculature by a carbohydrate mimetic peptide (2011)
  • Author(s): Hatakeyama S, Sugihara K, Shibata TK, Nakayama J, Akama TO, Tamura N, Wong S, Bobkov AA, Takano Y, Ohyama C, Fukuda M, Fukuda MN
  • Journal Title: Proc Natl Acad Sci USA Volume: 108 Issue: 49 Pages: 19587-19592
  • DOI: 10.1073/pnas.1105057108
  • Peer Reviewed
• [Presentation] C1 esterase inhibitor activity in amniotic fluid embolism (2014)
  • Author(s): Kanayama N, Tamura N, Itho H, Kashiwagi Y, Todo Y, Suzuki T, Yaguchi C
  • Organizer: SGI 61st Annual Scientific Meeting
  • Place of Presentation: Italy
• [Presentation] 臨床的羊水塞栓症における補体C3、C4測定の意義 (2012)
  • Author(s): 田村直顕 木村聡 森島賀子 内田季之 鈴木一有 杉村基 伊東宏晃 金山尚裕
  • Organizer: 第116回 日本産科麻酔科学会
  • Place of Presentation: 埼玉大宮
• [Presentation] 妊娠中の補体C1インヒビター活性の推移 (2012)
  • Author(s): 田村直顕 松田秀雄 吉田昌史 池田智明 下山華 村上浩雄 加藤誠 内田季之 鈴木一有 伊東宏晃 杉原一廣 金山尚裕
  • Organizer: 第64回 日本産科婦人科学会学術講演会
  • Place of Presentation: 神戸
• [Presentation] 羊水塞栓症登録事業のまとめ2010ー心肺虚脱初発群と出血DIC初発群の血清マーカーおよび患者背景の比較検討ー (2012)
  • Author(s): 木村聡 田村直顕 内田季之 鈴木一有 伊東宏晃 金山尚裕
  • Organizer: 第64回 日本産科婦人科学会学術講演会
  • Place of Presentation: 神戸
• [Presentation] 補助的な薬剤によるフォトフィリンPDTの抗腫瘍効果増強の検討 (2012)
  • Author(s): 村上浩雄 望月亜矢子 田村直顕 加藤誠 宮部勇樹 伊東宏晃 杉原一廣 金山尚裕
  • Organizer: 第64回 日本産科婦人科学会学術講演会
  • Place of Presentation: 神戸
• [Presentation] 母体血中、臍帯血中、羊水中の微量金属濃度について (2012)
  • Author(s): 内田季之 下山華 長橋ことみ 中村友紀 田村直顕 加藤誠 鈴木一有 杉原一廣 伊東宏晃 金山尚裕
  • Organizer: 第64回 日本産科婦人科学会学術講演会
  • Place of Presentation: 神戸
• [Presentation] 羊水混濁における免疫組織学的診断に関する検討 (2012)
  • Author(s): 古田直美 谷口千津子 村松慧子 山崎香織 長橋ことみ 中村友紀 田村直顕 内田季之 鈴木一有 杉原一廣 伊東宏晃 金山尚裕
  • Organizer: 第64回 日本産科婦人科学会学術講演会
  • Place of Presentation: 神戸
• [Presentation] 成熟嚢胞性奇形種より発生した卵巣粘表皮癌の一例 (2012)
  • Author(s): 田村直顕 川合健太 加藤誠 村上浩雄 平井強 児嶋裕香 安立匡志 福嶋真由 馬場聡 伊東宏晃 杉原一廣 金山尚裕
  • Organizer: 第53回 日本婦人科腫瘍学会学術講演会
  • Place of Presentation: 岡山
• [Presentation] The analysis of 303 cases with postpartum hemorrhage more than 2000 ml (2012)
  • Author(s): Tamura N, Itoh H, Uchida T, Suzuki K, Kanayama N
  • Organizer: 59th Annual Meeting of the Society for Gynecologic Investigation (SGI)
  • Place of Presentation: San Diego, USA
• [Presentation] Infantile growth after indernourishment in utero positively correlates with fat deposit in the liver of adult mice, as a risk of Non-alcoholic Fatty Liver Disease (NAFLD). (2012)
  • Author(s): Muramatsu K, Itoh H, Kohmura Y, Yamazaki K, Nagahashi K, Nakamura Y, Tamura N, Uchida T, Suzuki K, Kanayama N.
  • Organizer: 59th Annual Meeting of the Society for Gynecologic Investigation (SGI)
  • Place of Presentation: San Diego, USA
• [Presentation] Real time cerebral hemodynamics evalution by Time-Resolved Spectroscopy (TRS-20) during cesarean operation. (2012)
  • Author(s): Yamazaki K, Itoh H, Muramatsu K, Nagahashi K, Nakamura Y, Tamura N, Uchida T, Suzuki K, Kanayama N.
  • Organizer: 59th Annual Meeting of the Society for Gynecologic Investigation (SGI)
  • Place of Presentation: San Diego, USA
• [Presentation] Infantile growth after indernourishment in utero positively correlates with chronic inflammatory reactions in the white adipose tissues of adult mice, as a risk of developing obesity. (2012)
  • Author(s): Itoh H, Kohmura Y, Muramatsu K, Yamazaki K, Nagahashi K, Nakamura Y, Tamura N, Uchida T, Suzuki K, Kanayama N.
  • Organizer: 59th Annual Meeting of the Society for Gynecologic Investigation (SGI)
  • Place of Presentation: San Diego, USA
• [Presentation] Successful management of deliveries of a woman with PAI-1 deficiency -A critical role of PAI-1 in the maintenance of pregnancy- (2012)
  • Author(s): Nagahashi K, Iwaki T, Muramatsu K, Yamazaki K, Nakamura Y, Tamura N, Itoh H, Kanayama N.
  • Organizer: 59th Annual Meeting of the Society for Gynecologic Investigation (SGI)
  • Place of Presentation: San Diego, USA
• [Presentation] Trophinin-mediated cell adhesion induces apoptosis of human endometrial epithelial cells through PKC-δ (2011)
  • Author(s): Tamura N
  • Organizer: The 11 th Hamamatsu-Kyungpook Joint Medical Symposium
  • Place of Presentation: Korea
• [Presentation] Trophinin-mediated cell adhesion induces apoptosis of human endometrial epithelial cells through PKC-δ. (2011)
  • Author(s): Tamura N
  • Organizer: The 11th Hamamatsu-Kyungpook Joint Medical Symposium
  • Place of Presentation: Kyungpook, Korea
• [Presentation] トロフィニンによる細胞接着はPKC-δを介して子宮内膜上皮細胞のアポトーシスを誘導する. (2011)
  • Author(s): 田村直顕、杉原一廣、伊東宏晃、金山尚裕
  • Organizer: 日本産科婦人科学会 第63回学術総会
  • Place of Presentation: 大阪
• [Book] 今日の治療指針 子宮内胎児死亡 (2013)
  • Author(s): 子宮内胎児死亡
  • Publisher: 医学書院
• [Book] 臨床婦人科産科【早産の予防・診断】早産と細菌性腟症 (2011)
  • Author(s): 田村直顕，伊東宏晃
  • Publisher: 医学書院
• [Book] 周産期医学必修知識 第7版 産科DIC (2011)
  • Author(s): 田村直顕，金山尚裕
  • Publisher: 東京医学社
• [Book] 早産 産婦人科学レビュー＜最新主要文献と解説＞ (2011)
  • Author(s): 田村直顕，金山尚裕
  • Publisher: 総合医学社