Identification and functional analysis of microRNAs involved in the onset of proteinurea accompanied with pregnancy-induced hypertension

• Project/Area Number: 23592419
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Osaka Prefecture University (2012-2013); Nippon Medical School (2011)
• Principal Investigator: ISHIBASHI OSAMU 大阪府立大学, 生命環境科学研究科(系), 准教授 (70293214)
• Co-Investigator(Kenkyū-buntansha): 瀧澤 俊広 日本医科大学, 医学(系)研究科(研究院), 教授 (90271220) ; 竹下 俊行 日本医科大学, 医学(系)研究科(研究院), 教授 (60188175)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 妊娠高血圧症候群 / ポドサイト / マイクロRNA / 次世代シーケンス / 蛋白尿 / RNA-seq / 胎盤 / マイクロアレイ / エンドセリン

Research Abstract

This study was conducted in order to elucidate the molecular mechanism of proteinurea onset accompanied with pregnancy-induced hypertension (PIH). Primary podocytes (glomerular epithelial cells) were treated with endothelin-1 to induce cellular injury to the cells, whereby an in vitro pathological model reflecting the PIH-related proteinurea was established. Further, transcripts (including non-coding RNAs) dysregulated in accordance with the podocyte injury were explored in a genome-wide fashion by means of next-generation sequencing and PCR-based microRNA array system.

Research Products

• [Journal Article] Dataset of microarray analysis to identify endoglin-dependent bone morphogenetic protein-2-responsive genes in the murine periodontal ligament cell line PDL-L2 (2014)
  • Author(s): Ishibashi, O., Inui, T
  • Journal Title: Genomics Data Volume: 2巻 Pages: 24-26
  • Peer Reviewed
• [Journal Article] Interactions between the non-seed region of siRNA and RNA-binding RLC/RISC proteins, Ago and TRBP, in mammalian cells (2014)
  • Author(s): Takahashi, T., Zenno, S., Ishibashi, O., Takizawa, T., Saigo, K., Ui-Tei, K
  • Journal Title: Nucleic Acids Res Volume: 42巻 Pages: 5256-5269
  • Peer Reviewed
• [Journal Article] Interactions between the non-seed region of siRNA and RNA-binding RLC/RISC proteins, Ago and TRBP, in mammalian cells. (2014)
  • Author(s): Takahashi T, Zenno S, Ishibashi O, Takizawa T, Saigo K, Ui-Tei K.
  • Journal Title: Nucleic Acids Res. Volume: 42 Issue: 8 Pages: 5256-5269
  • DOI: 10.1093/nar/gku153
  • Peer Reviewed / Open Access
• [Journal Article] Dataset of microarray analysis to identify endoglin-dependent bone morphogenetic protein-2-responsive genes in the murine periodontal ligament cell line PDL-L2. (2014)
  • Author(s): Ishibashi O, Inui T
  • Journal Title: Genomics Data Volume: 2 Pages: 24-26
  • DOI: 10.1016/j.gdata.2014.03.002
  • Peer Reviewed
• [Journal Article] BMP-2 desensitizes MC3T3-E1 osteoblastic cells to estrogen through transcriptional downregulation of estrogen receptor 1 (2013)
  • Author(s): Ishibashi, O
  • Journal Title: J. Bone Metab Volume: 20巻 Pages: 83-88
  • Peer Reviewed
• [Journal Article] Bone Morphogenetic Protein-2 Desensitizes MC3T3-E1 Osteoblastic Cells to Estrogen Through Transcriptional Downregulation of Estrogen Receptor 1. (2013)
  • Author(s): Ishibashi O
  • Journal Title: Journal of Bone Metabolism Volume: 20 Issue: 2 Pages: 83-88
  • DOI: 10.11005/jbm.2013.20.2.83
  • Peer Reviewed
• [Journal Article] Plasma level of hydroxysteroid (17-β) dehydrogenase 1 in the second trimester is an independent risk factor for predicting preeclampsia after adjusting for the effects of mean blood pressure, bilateral notching and plasma level of soluble fms-like tyrosine kinase 1/placental growth factor ratio (2012)
  • Author(s): Ohkuchi, A., Ishibashi, O., Hirashima, C., Takahashi, K., Matsubara, S., Takizawa, T., Suzuki, M. (共筆頭著者)
  • Journal Title: Hypertens. Res Volume: 35巻 Pages: 1152-1158
  • Peer Reviewed
• [Journal Article] MiR-21 is enriched in the RNA-induced silencing complex and targets COL4A1 in human granulosa cell lines (2012)
  • Author(s): Mase, Y., Ishibashi, O., Ishikawa, T., Takizawa, T., Kiguchi, T., Ohba, T., Katabuchi, H., Takeshita, T., Takizawa, T
  • Journal Title: Reprod. Sci. Volume: 19巻 Pages: 1030-1040
  • Peer Reviewed
• [Journal Article] Hydroxysteroid (17-beta) dehydrogenase 1 is dysregulated by mir-210 and mir-518c that are aberrantly expressed in preeclamptic placentas : A novel marker for predicting preeclampsia (2012)
  • Author(s): Ishibashi, O., Ohkuchi, A., Ali, M.M., Kurashina, R., Luo, S-S., Ishikawa, T., Takizawa, T., Hirashima, C, Takahashi, K., Migita, M., Ishikawa, G., Yoneyama, K., Asakura, H., Izumi, A., Matsubara,S., Takeshita, T., Takizawa, T.
  • Journal Title: Hypertension Volume: 59巻 Pages: 265-273
  • Peer Reviewed
• [Journal Article] Hydroxysteroid(17-β) dehydrogenase 1 is dysregulated by miR-210 and miR-518c that are aberrantly expressed in preeclamptic placentas : a novel marker for predicting preeclampsia (2012)
  • Author(s): Ishibashi O, Ohkuchi A, Ali MM, Kurashina R, Luo SS, Ishikawa T, Takizawa T, Hirashima C, Takahashi K, Migita M, Ishikawa G, Yoneyama K, Asakura H, Izumi A, Matsubara S, Takeshita T, Takizawa T
  • Journal Title: Hypertension Volume: 59 Issue: 2 Pages: 265-273
  • DOI: 10.1161/hypertensionaha.111.180232
  • Peer Reviewed
• [Journal Article] Short RNA duplexes elicit apoptosis via the sequential activation of PKR, RIG-I, and p38 in a cell type- and length-dependent manner (2011)
  • Author(s): Ishibashi, O., Ali, M.M., Luo, S-S., Ohba, T., Katabuchi, H., Takeshita, T., Takizawa, T.
  • Journal Title: Sci. Signal Volume: 4巻
  • Peer Reviewed
• [Journal Article] Real-time PCR-based microRNAome of human bile detects miR-9 as a potential diagnostic biomarker for biliary tract cancer (2011)
  • Author(s): Shigehara, K., Yokomuro, S., Ishibashi, O., Mizuguchi, Y., Arima, Y., Kawahigashi, Y., Kanda, T., Akagi, I., Tajiri, T., Takizawa, T., Yoshida, H., Uchida, E
  • Journal Title: PLoS ONE Volume: 6巻
  • Peer Reviewed
• [Presentation] マウス成熟破骨細胞の大規模トランスクリプトーム解析と遺伝子発現プロファイリング (2013)
  • Author(s): 小谷康斗, 他
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸(兵庫)
  • Year and Date: 2013-12-05
• [Presentation] マウス歯根膜細胞におけるBMP2に対するendoglin依存的応答遺伝子の探索 (2013)
  • Author(s): 石橋宰, 他
  • Organizer: 第86回日本生化学会
  • Place of Presentation: 横浜(神奈川)
  • Year and Date: 2013-09-13
• [Presentation] マウス歯根膜細胞におけるBMP2に対するendoglin依存的応答遺伝子の探索 (2013)
  • Author(s): 石橋 宰、乾 隆
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜国際会議場
• [Presentation] エクソソームmicroRNAを介した胎盤-リンパ球間コミュニケーション:培養細胞を用いたモデル解析 (2011)
  • Author(s): アリモハメド, 他
  • Organizer: 第27回日本生殖免疫学会
  • Place of Presentation: 名古屋(愛知)
  • Year and Date: 2011-12-03
• [Presentation] 妊婦血漿中でのHSD17B1の検出と妊娠高血圧腎症発症例におけるその動態 (2011)
  • Author(s): 石橋宰, 他
  • Organizer: 第32回日本妊娠高血圧学会
  • Place of Presentation: 金沢(石川)
  • Year and Date: 2011-10-22
• [Presentation] 妊婦血漿中での HSD17B1 の検出と妊娠高血圧腎症発症例におけるその動態 (2011)
  • Author(s): 石橋 宰
  • Organizer: 妊娠高血圧学会
  • Place of Presentation: 金沢歌劇座（石川県）
• [Remarks]
  • URL: http://www.bioinfo.osakafu-u.ac.jp/~inuit/
• [Remarks] 生体高分子機能学
  • URL: http://www.bioinfo.osakafu-u.ac.jp/~inuit/Ishibashi.html
• [Remarks] 大阪府立大学生体高分子機能学ホームページ