Identification of potential marker of cancer stem cell from ovarian clear cell carcinoma
Project/Area Number |
23592471
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Obstetrics and gynecology
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Research Institution | Tohoku University |
Principal Investigator |
SUZUKI KICHIYA 東北大学, 東北メディカル・メガバンク機構, 准教授 (30422116)
|
Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Kazunori 宮城県立がんセンター, 発がん制御研究部, 主任研究員 (80373215)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 卵巣癌 / がん幹細胞 / 表面マーカー / 免疫不全マウス / フローサイトメトリー |
Research Abstract |
Ovarian cancer frequently demonstrates chemotherapy-resistant mechanism and it has been recently reported that cancer stem cell is involved in this mechanism. We have planned to determine potential marker for cancer stem cell of ovarian cancer. We have chosen two ovarian cancer cell lines out of nine ovarian cancer cell lines based on tumor growth activity using immune deficient mice experiment (NOG mice). We have searched several potential marker for cancer stem cell and found that EpCAM negative population demonstrates high growth rate when inoculated to NOG mice. We have established EpCAM knock-down cell lines under doxycycline control. Unexpectedly the cell lines demonstrate growth inhibition when EpCAM expression was decresed. These data obtained from in vivo and in vitro experiments show some discrepancy however the mechanism is still unclear.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Suppressive expression of CD274 increases tumorigenesis and cancer stem cell phenotypes in cholangiocarcinoma.2014
Author(s)
Tamai K, Nakamura M, Mizuma M, Mochizuki M, Yokoyama M, Endo H, Yamaguchi K, Nakagawa T, Shiina M, Unno M, Muramoto K, Sato I, Satoh K, Sugamura K, Tanaka N.
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Journal Title
Cancer Sci
Volume: Mar 26
Related Report
Peer Reviewed
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[Journal Article] Decreased Expression of 14-3-3<i>σ</i> Is Predictive of Poor Prognosis for Patients with Human Uterine Papillary Serous Carcinoma2013
Author(s)
1. Suzuki F, Nagase S, Suzuki K, Oba E, Hiroki E, Matsuda Y, Akahira J, Nishigori H, Sugiyama T, Otsuki T, Yoshinaga K, Takano T, Niikura H, Ito K, Sasano H, and Yaegashi N
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Journal Title
The Tohoku Journal of Experimental Medicine
Volume: 231
Issue: 3
Pages: 193-199
DOI
NAID
ISSN
0040-8727, 1349-3329
Related Report
Peer Reviewed
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[Journal Article] Molecular cloning and biochemical characterization of two novel Neu3 sialidases, neu3a and neu3b, from medaka (Oryzias latipes)2013
Author(s)
Shiozaki K, Takeshita K, Ikeda M, Ikeda A, Harasaki Y, Komatsu M, Yamada S, Yamaguchi K, Miyagi T
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Journal Title
Biochimie
Volume: 95
Issue: 2
Pages: 280-289
DOI
Related Report
Peer Reviewed
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[Journal Article] Decreased Expression of 14-3-3σ is Predictive of Poor Prognosis for Patients with Human Uterine Papillary Serous Carcinoma.2013
Author(s)
Suzuki F, Nagase S, Suzuki K, Oba E, Hiroki E, Matsuda Y, Akahira J, Nishigori H, Sugiyama T, Otsuki T, Yoshinaga K, Takano T, Niikura H, Ito K, Sasano H, and Yaegashi N.
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Journal Title
Tohoku J Exp Med
Volume: 231
Pages: 193-199
NAID
Related Report
Peer Reviewed
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[Journal Article] Enhanced expression of long non-coding RNA HOTAIR is associated with the development of gastric cancer.2013
Author(s)
Endo H, Shiroki T, Nakagawa T, Yokoyama M, Tamai K, Yamanami H, Fujiya T, Sato I, Yamaguchi K, Tanaka N, Iijima K, Shimosegawa T, Sugamura K, Satoh K.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] CD271 defines a stem cell-like population in hypopharyngeal cancer.2013
Author(s)
Imai T, Tamai K, Oizumi S, Oyama K, Yamaguchi K, Sato I, Satoh K, Matsuura K, Saijo S, Sugamura K, Tanaka N.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] DNA demethylation-dependent AR recruitment and GATA factors drive Rhox5 homeobox gene transcription in the epididymis2012
Author(s)
Bhardwaj A, Song HW, Beildeck M, Kerkhofs S, Castoro R, Shanker S, De Gendt K, Suzuki K, Claessens F, Issa JP, Orgebin-Crist MC, Wilkinson MF.
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Journal Title
Mol Endocrinol
Volume: 26
Issue: 4
Pages: 538-49
DOI
Related Report
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[Journal Article] Sialidase NEU4 hydrolyzes polysialic acids of neural cell adhesion molecules and negatively regulates neurite formation by hippocampal neurons. 862012
Author(s)
Takahashi K, Mitoma J, Hosono M, Shiozaki K, Sato C, Yamaguchi K, Kitajima K, Higashi H, Nitta K, Shima H, Miyagi T
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Journal Title
J Biol Chem
Volume: 287
Pages: 14816-14826
Related Report
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[Journal Article] DNA demethylation-dependent AR recruitment and GATA factors drive Rhox5 homeobox gene transcription in the epididymis.2012
Author(s)
Bhardwaj A, Song HW, Beildeck M, Kerkhofs S, Castoro R, Shanker S, De Gendt K, Suzuki K, Claessens F, Issa JP, Orgebin-Crist MC, Wilkinson MF
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Journal Title
Mol Endocrinol
Volume: 26
Pages: 538-549
Related Report
Peer Reviewed
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[Journal Article] Molecular cloning and biochemical characterization of two novel Neu3 sialidases, neu3a and neu3b, from medaka (Oryzias latipes).2012
Author(s)
Shiozaki K, Takeshita K, Ikeda M, Ikeda A, Harasaki Y, Komatsu M, Yamada S, Yamaguchi K, Miyagi T.
-
Journal Title
Biochimie
Volume: 95
Pages: 280-289
Related Report
Peer Reviewed
-
[Journal Article] Sialidase NEU4 hydrolyzes polysialic acids of neural cell adhesion molecules and negatively regulates neurite formation by hippocampal neurons.2012
Author(s)
Takahashi K, Mitoma J, Hosono M, Shiozaki K, Sato C, Yamaguchi K, Kitajima K, Higashi H, Nitta K, Shima H, Miyagi T.
-
Journal Title
J Biol Chem
Volume: 287
Pages: 14816-14826
Related Report
Peer Reviewed
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[Journal Article] NA Demethylation-Dependent AR Recruitment and GATA Factors Drive Rhox5 Homeobox Gene Transcription in the Epididymis2012
Author(s)
Bhardwaj A, Song HW, Beildeck M, Kerkhofs S, Castoro R, Shanker S, De Gendt K, Suzuki K, Claessens F, Issa JP, Orgebin-Crist MC, and Wilkinson MF
-
Journal Title
Mol Endocrinol.
Volume: 26
Pages: 538-549
Related Report
Peer Reviewed
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