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Identification of potential marker of cancer stem cell from ovarian clear cell carcinoma

Research Project

Project/Area Number 23592471
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Obstetrics and gynecology
Research InstitutionTohoku University

Principal Investigator

SUZUKI KICHIYA  東北大学, 東北メディカル・メガバンク機構, 准教授 (30422116)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI Kazunori  宮城県立がんセンター, 発がん制御研究部, 主任研究員 (80373215)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords卵巣癌 / がん幹細胞 / 表面マーカー / 免疫不全マウス / フローサイトメトリー
Research Abstract

Ovarian cancer frequently demonstrates chemotherapy-resistant mechanism and it has been recently reported that cancer stem cell is involved in this mechanism. We have planned to determine potential marker for cancer stem cell of ovarian cancer. We have chosen two ovarian cancer cell lines out of nine ovarian cancer cell lines based on tumor growth activity using immune deficient mice experiment (NOG mice). We have searched several potential marker for cancer stem cell and found that EpCAM negative population demonstrates high growth rate when inoculated to NOG mice. We have established EpCAM knock-down cell lines under doxycycline control. Unexpectedly the cell lines demonstrate growth inhibition when EpCAM expression was decresed. These data obtained from in vivo and in vitro experiments show some discrepancy however the mechanism is still unclear.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (26 results)

All 2014 2013 2012 2011

All Journal Article (25 results) (of which Peer Reviewed: 20 results) Book (1 results)

  • [Journal Article] Suppressive expression of CD274 increases tumorigenesis and cancer stem cell phenotypes in cholangiocarcinoma.2014

    • Author(s)
      Tamai K, Nakamura M, Mizuma M, Mochizuki M, Yokoyama M, Endo H, Yamaguchi K, Nakagawa T, Shiina M, Unno M, Muramoto K, Sato I, Satoh K, Sugamura K, Tanaka N.
    • Journal Title

      Cancer Sci

      Volume: Mar 26

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Decreased Expression of 14-3-3<i>σ</i> Is Predictive of Poor Prognosis for Patients with Human Uterine Papillary Serous Carcinoma2013

    • Author(s)
      1. Suzuki F, Nagase S, Suzuki K, Oba E, Hiroki E, Matsuda Y, Akahira J, Nishigori H, Sugiyama T, Otsuki T, Yoshinaga K, Takano T, Niikura H, Ito K, Sasano H, and Yaegashi N
    • Journal Title

      The Tohoku Journal of Experimental Medicine

      Volume: 231 Issue: 3 Pages: 193-199

    • DOI

      10.1620/tjem.231.193

    • NAID

      130004720755

    • ISSN
      0040-8727, 1349-3329
    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning and biochemical characterization of two novel Neu3 sialidases, neu3a and neu3b, from medaka (Oryzias latipes)2013

    • Author(s)
      Shiozaki K, Takeshita K, Ikeda M, Ikeda A, Harasaki Y, Komatsu M, Yamada S, Yamaguchi K, Miyagi T
    • Journal Title

      Biochimie

      Volume: 95 Issue: 2 Pages: 280-289

    • DOI

      10.1016/j.biochi.2012.09.026

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Decreased Expression of 14-3-3σ is Predictive of Poor Prognosis for Patients with Human Uterine Papillary Serous Carcinoma.2013

    • Author(s)
      Suzuki F, Nagase S, Suzuki K, Oba E, Hiroki E, Matsuda Y, Akahira J, Nishigori H, Sugiyama T, Otsuki T, Yoshinaga K, Takano T, Niikura H, Ito K, Sasano H, and Yaegashi N.
    • Journal Title

      Tohoku J Exp Med

      Volume: 231 Pages: 193-199

    • NAID

      130004720755

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced expression of long non-coding RNA HOTAIR is associated with the development of gastric cancer.2013

    • Author(s)
      Endo H, Shiroki T, Nakagawa T, Yokoyama M, Tamai K, Yamanami H, Fujiya T, Sato I, Yamaguchi K, Tanaka N, Iijima K, Shimosegawa T, Sugamura K, Satoh K.
    • Journal Title

      PLoS One

      Volume: 8

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CD271 defines a stem cell-like population in hypopharyngeal cancer.2013

    • Author(s)
      Imai T, Tamai K, Oizumi S, Oyama K, Yamaguchi K, Sato I, Satoh K, Matsuura K, Saijo S, Sugamura K, Tanaka N.
    • Journal Title

      PLoS One

      Volume: 8

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA demethylation-dependent AR recruitment and GATA factors drive Rhox5 homeobox gene transcription in the epididymis2012

    • Author(s)
      Bhardwaj A, Song HW, Beildeck M, Kerkhofs S, Castoro R, Shanker S, De Gendt K, Suzuki K, Claessens F, Issa JP, Orgebin-Crist MC, Wilkinson MF.
    • Journal Title

      Mol Endocrinol

      Volume: 26 Issue: 4 Pages: 538-49

    • DOI

      10.1210/me.2011-1059

    • Related Report
      2013 Final Research Report
  • [Journal Article] Plasma membrane-associated sialidase (NEU3) regulates progression of prostate cancer to androgen-independent growth through modulation of androgen receptor signaling2012

    • Author(s)
      Kawamura, S., et al
    • Journal Title

      Cell Death Differ

      Volume: 19 Issue: 1 Pages: 170-179

    • DOI

      10.1038/cdd.2011.83

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structure, Biosynthesis and Function Sialic Acid Glycoconjugates in Health and Disease, 2012, 188-208 Joe Tiralongo and Ivan Martinez-Duncker (Eds)2012

    • Author(s)
      Suzuki T, Yamaguchi K
    • Journal Title

      Bentham Science Publishers CHAPTER 6 Mammalian Sialidases.

    • Related Report
      2013 Final Research Report
  • [Journal Article] Sialidase NEU4 hydrolyzes polysialic acids of neural cell adhesion molecules and negatively regulates neurite formation by hippocampal neurons. 862012

    • Author(s)
      Takahashi K, Mitoma J, Hosono M, Shiozaki K, Sato C, Yamaguchi K, Kitajima K, Higashi H, Nitta K, Shima H, Miyagi T
    • Journal Title

      J Biol Chem

      Volume: 287 Pages: 14816-14826

    • Related Report
      2013 Final Research Report
  • [Journal Article] Mammalian sialidases : physiological and pathological roles in cellular functions2012

    • Author(s)
      Miyagi, T., et al
    • Journal Title

      Glycobiology

      Volume: 22 Issue: 7 Pages: 880-896

    • DOI

      10.1093/glycob/cws057

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reduced susceptibility to colitis-associated colon carcinogenesis in mice lacking plasma membrane-associated sialidase2012

    • Author(s)
      Yamaguchi K, Shiozaki K, Moriya S, Koseki K, Wada T, Tateno H, Sato I, Asano M, Iwakura Y, Miyagi T
    • Journal Title

      PLoS One

      Volume: 7

    • Related Report
      2013 Final Research Report
  • [Journal Article] Altered expression of sialidases in human cancer2012

    • Author(s)
      Miyagi T, Takahashi K, Moriya S, Hata K, Yamamoto K, Wada T, Yamaguchi K, Shiozaki K
    • Journal Title

      Adv Exp Med Biol

      Volume: 749 Pages: 257-267

    • DOI

      10.1007/978-1-4614-3381-1_17

    • ISBN
      9781461433804, 9781461433811
    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human cytosolic sialidase NEU2-low general tissue expression but involvementin PC-3 prostate cancer cell survival2012

    • Author(s)
      Koseki K, Wada T, Hosono M, Hata K, Yamaguchi K, Nitta K, Miyagi T
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 428 Issue: 1 Pages: 142-149

    • DOI

      10.1016/j.bbrc.2012.10.028

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] DNA demethylation-dependent AR recruitment and GATA factors drive Rhox5 homeobox gene transcription in the epididymis.2012

    • Author(s)
      Bhardwaj A, Song HW, Beildeck M, Kerkhofs S, Castoro R, Shanker S, De Gendt K, Suzuki K, Claessens F, Issa JP, Orgebin-Crist MC, Wilkinson MF
    • Journal Title

      Mol Endocrinol

      Volume: 26 Pages: 538-549

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Reduced susceptibility to colitis-associated colon carcinogenesis in mice lacking plasma membrane-associated sialidase.2012

    • Author(s)
      Yamaguchi K, Shiozaki K, Moriya S, Koseki K, Wada T, Tateno H, Sato I, Asano M, Iwakura Y, Miyagi T.
    • Journal Title

      PLoS One

      Volume: 7

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning and biochemical characterization of two novel Neu3 sialidases, neu3a and neu3b, from medaka (Oryzias latipes).2012

    • Author(s)
      Shiozaki K, Takeshita K, Ikeda M, Ikeda A, Harasaki Y, Komatsu M, Yamada S, Yamaguchi K, Miyagi T.
    • Journal Title

      Biochimie

      Volume: 95 Pages: 280-289

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Sialidase NEU4 hydrolyzes polysialic acids of neural cell adhesion molecules and negatively regulates neurite formation by hippocampal neurons.2012

    • Author(s)
      Takahashi K, Mitoma J, Hosono M, Shiozaki K, Sato C, Yamaguchi K, Kitajima K, Higashi H, Nitta K, Shima H, Miyagi T.
    • Journal Title

      J Biol Chem

      Volume: 287 Pages: 14816-14826

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Altered expression of sialidases in human cancer.2012

    • Author(s)
      Miyagi T, Takahashi K, Moriya S, Hata K, Yamamoto K, Wada T, Yamaguchi K, Shiozaki K.
    • Journal Title

      Adv Exp Med Biol

      Volume: 749 Pages: 257-267

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Sialidase significance for cancer progression.2012

    • Author(s)
      Miyagi T, Takahashi K, Hata K, Shiozaki K, Yamaguchi K.
    • Journal Title

      Glycoconj J

      Volume: 29 Pages: 567-577

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mammalian sialidases: physiological and pathological roles in cellular functions.2012

    • Author(s)
      Miyagi T, Yamaguchi K.
    • Journal Title

      Glycobiology

      Volume: 22 Pages: 880-896

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Human cytosolic sialidase NEU2-low general tissue expression but involvementin PC-3 prostate cancer cell survival.2012

    • Author(s)
      Koseki K, Wada T, Hosono M, Hata K, Yamaguchi K, Nitta K, Miyagi T.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 428 Pages: 142-149

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] NA Demethylation-Dependent AR Recruitment and GATA Factors Drive Rhox5 Homeobox Gene Transcription in the Epididymis2012

    • Author(s)
      Bhardwaj A, Song HW, Beildeck M, Kerkhofs S, Castoro R, Shanker S, De Gendt K, Suzuki K, Claessens F, Issa JP, Orgebin-Crist MC, and Wilkinson MF
    • Journal Title

      Mol Endocrinol.

      Volume: 26 Pages: 538-549

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Regulation of Sialyl Lewis Antigen Expression in Colon Cancer Cells by Sialidase NEU42011

    • Author(s)
      Shiozaki, K., et al
    • Journal Title

      J Biol Chem

      Volume: 286 Issue: 24 Pages: 21052-21061

    • DOI

      10.1074/jbc.m111.231191

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] シアリダーゼ、生物機能モデルと新しいリソースリサーチツール2011

    • Author(s)
      山口壹範,宮城妙子
    • Journal Title

      エルアイシー

      Pages: 340-345

    • URL

      http://homepage3.nifty.com/lic/books/nbs09.html

    • Related Report
      2013 Final Research Report
  • [Book] Structure, Biosynthesis and Function Sialic Acid Glycoconjugates in Health and Disease in Mammalian Sialidases CHAPTER 6 (Eds Tiralongo J and Martinez-Duncker I)2012

    • Author(s)
      Suzuki T and Yamaguchi K.
    • Publisher
      Bentham Science Publishers
    • Related Report
      2012 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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