| Project/Area Number |
23592524
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
MISAWA Kiyoshi 浜松医科大学, 医学部附属病院, 講師 (90334979)
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| Co-Investigator(Kenkyū-buntansha) |
MINETA Hiroyuki 浜松医科大学, 医学部, 教授 (40190714)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 癌幹細胞 / 頭頸部癌 / メチル化 / 癌幹細胞マーカー |
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| Research Abstract |
The polycomb proteins BMI1 and EZH2 are characteristic components of the PRC1 and PRC2 repressor complexes, respectively, that modify chromatin. The polycomb proteins are overexpressed in several human malignancies. Moreover, BMI1 and EZH2 may influence DNA methylation by direct interaction with DNA methyltransferases. BMI1 and EZH2 expression were examined using quantitative RT-PCR. The methylation status of 9 genes was studied using methylation-specific PCR.Aberrant EZH2 and BMI1 mRNA expression was observed in 27 (29.7%) and 22 (24.2%), respectively. Aberrant BMI1 and EZH2 expression was significantly associated with shorter disease-free survival of the patients (P = .031 and P = .028, respectively). In addition, aberrant BMI1 expression was associated with DNA hypermethylation in HNSCC tissues. Futhermore, we show that histone deacetylase inhibitors downregulate expression of BMI1 and EZH2.It is conceivable that inhibitors of BMI1 and EZH2 are likely to have high therapeutic value.
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