A study on the role of Tropomyosin gene for cataractogenesis and the prevention of cataract

• Project/Area Number: 23592558
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Kanazawa Medical University
• Principal Investigator: KUBO Eri 金沢医科大学, 医学部, 教授 (10262619)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: トロポミオシン / 白内障 / 後嚢混濁 / 細胞分化 / 上皮間葉系移行 / 水晶体上皮細胞 / 後発白内障 / FGF2 / TGFβ2 / マイクロRNA

Research Abstract

Tropomyosin is an extracellular matrix protein related with differentiation of lens.Our study revealed that Tpm is related with the onset of human anterior subcapsular cataract and severe nuclear cataract. Further, Tpm was also related with the progression of posterior capsular opacity after cataract, which induces the impaired visual function.
From these results, Tpm may be an important gene related with lens differentiation and epithelial mesenchymal transition.

Research Products

• [Journal Article] Dynamic and differential regulation in the microRNA expression in the developing and mature cataractous rat lens (2013)
  • Author(s): Kubo E, Hasanova N, Sasaki H, Singh DP
  • Journal Title: J Cell Mol Med Volume: 17 Pages: 1146-1159
  • Peer Reviewed
• [Journal Article] Elevated tropomyosin expression is associated with epithelial-mesenchymal transition of lens epithelial cells (2013)
  • Author(s): Kubo E, Hasanova N, Fatma N, Sasaki H, Singh DP
  • Journal Title: Cell Mol Med Volume: 17 Pages: 212-21
  • Peer Reviewed
• [Journal Article] Dynamic and differential regulation in the microRNA expression in the developing and mature cataractous rat lens. (2013)
  • Author(s): Kubo E, Hasanova N, Sasaki H, Singh DP.
  • Journal Title: Journal of Cellular and Molecular Medicine Volume: 17 Issue: 9 Pages: 1146-1159
  • DOI: 10.1111/jcmm.12094
  • Peer Reviewed
• [Journal Article] Elevated tropomyosin expression is associated with epithelial-mesenchymal transition of lens epithelial cells. (2013)
  • Author(s): Kubo E, Hasanova N, Fatma N, Sasaki H, Singh DP.
  • Journal Title: Journal of Cellular and Molecular Medicine Volume: 17 Issue: 1 Pages: 212-221
  • DOI: 10.1111/j.1582-4934.2012.01654.x
  • Peer Reviewed
• [Presentation] 白内障術後の水晶体上皮細胞の変化 (2014)
  • Author(s): 久保江理
  • Organizer: 第118回日本眼科学会総会
  • Place of Presentation: 東京(東京国際フォーラム、帝国ホテル)
  • Invited
• [Presentation] Tropomyosin : Its relationship with posterior capsule opacity (2014)
  • Author(s): Kubo E, Osada H, Shibata N, Kiyokawa E, Sasaki H, Singh DP
  • Organizer: International Conference of Lens 2014
  • Place of Presentation: Kona (アメリカ合衆国)
• [Presentation] Tropomyosin: Its relationship to posterior capsule opacity (2014)
  • Author(s): Eri Kubo, Hiromi Osada, Naoko Shibata, Etsuko Kiyokaw, Hiroshi Sasaki, Dhirendra P Singh
  • Organizer: International Conference of Lens 2014
  • Place of Presentation: シェラトンコナ、ハワイ州、アメリカ合衆国
• [Presentation] 白内障術後の水晶体上皮細胞の変化 (2014)
  • Author(s): 久保江理
  • Organizer: 第118回日本眼科学会
  • Place of Presentation: 東京国際フォーラム、東京都千代田区
  • Invited
• [Presentation] 後発白内障にみる水晶体の再生と上皮間葉系移(EMT) (2013)
  • Author(s): 久保江理
  • Organizer: 第14回金沢医科大学研究セミナー
  • Place of Presentation: 金沢(金沢医科大学病院本館4階C41講義室)
  • Year and Date: 2013-09-30
• [Presentation] 白内障術後後嚢混濁におけるFGF2およびTGFβ2によるトロポミオシン発現制御機構 (2013)
  • Author(s): 長田ひろみ、ハサノワナイリア、柴田奈保子、清川悦子、佐々木洋、久保江理
  • Organizer: 第52回日本白内障学会総会
  • Place of Presentation: 浦安(シェラトングランデトーキョーベイホテル、ヒルトン東京ベイ)
• [Presentation] FGF2 Antagonizes the TGFβ2-Induced Aberrant Expression of Tropomyosin andα-Smooth Muscle Actin in Mouse and Human Lens Epithelial Cells and Has a Role in Posterior Capsule Opacity (2013)
  • Author(s): Kubo E, Hasanova N, Osada H, Kiyokawa E, Sasaki H, Singh DP
  • Organizer: ARVO 2013 Annual Meeting
  • Place of Presentation: Seattle (アメリカ合衆国)
• [Presentation] 後発白内障モデルを用いた薬物治療の可能性 (2013)
  • Author(s): 久保江理
  • Organizer: 第117回日本眼科学会総会
  • Place of Presentation: 東京(東京国際フォーラム)
  • Invited
• [Presentation] FGF2 Antagonizes the TGFβ2-Induced Aberrant Expression of Tropomyosin and α-Smooth (2013)
  • Author(s): Eri Kubo, Nailia Hasanova, Hiromi Osada, Etsuko Kiyokawa, Hiroshi Sasaki, Dhirendra P Singh
  • Organizer: ARVO 2013 Annual Meeting
  • Place of Presentation: ワシントン州コンベンションセンター、シアトル、 アメリカ合衆国
• [Presentation] 白内障術後後嚢混濁におけるFGF2およびTGFβ2によるトロポミオシン発現制御機構. (2013)
  • Author(s): 6. 長田ひろみ、ハサノワ ナイリア、柴田奈保子、清川悦子、佐々木洋、久保江理.
  • Organizer: 第52回日本白内障学会総会.
  • Place of Presentation: シェラトン・グランデ・トーキョーベイ・ホテル 千葉県浦安市
• [Presentation] 後発白内障にみる水晶体の再生と上皮間葉系移(EMT). (2013)
  • Author(s): 久保江理
  • Organizer: 第14回金沢医科大学研究セミナー.
  • Place of Presentation: 金沢医科大学、石川県河北郡内灘
  • Invited
• [Presentation] 後発白内障モデルを用いた薬物治療の可能性 (2013)
  • Author(s): 久保江理
  • Organizer: 第117回日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム
  • Invited
• [Presentation] FGF2 Antagonizes the TGFβ2-Induced Aberrant Expression of Tropomyosin and α-Smooth Muscle Actin in Mouse and Human Lens Epithelial Cells and Has a Role in Posterior Capsule Opacity (2013)
  • Author(s): Eri Kubo
  • Organizer: ARVO 2013 Annual Meeting
  • Place of Presentation: シアトル
• [Presentation] 白内障予防と治療の可能性 (2011)
  • Author(s): 久保江理
  • Organizer: 第31回金沢医科大学眼科研究会
  • Place of Presentation: 金沢(金沢市アートホール)
  • Year and Date: 2011-11-06
• [Presentation] 後発白内障にみる水晶体再生 (2011)
  • Author(s): 久保江理
  • Organizer: 第115回日本眼科学会総会
  • Place of Presentation: 東京(東京国際フォーラム)
• [Presentation] Aberrant Expression of Tropomyosin Causes Epithelial-Mesenchymal Transition of Lens Epithelial Cells (2011)
  • Author(s): Kubo E, Hasanova N, Singh DP, Takamura Y, Akagi Y
  • Organizer: ARVO 2010 Annual Meeting
  • Place of Presentation: Fort Lauderdale (アメリカ合衆国)
• [Presentation] 後発白内障にみる水晶体再生 (2011)
  • Author(s): 久保江理
  • Organizer: 第115回日本眼科学会総会(招待講演)
  • Place of Presentation: 東京国際フォーラム
• [Presentation] Aberrant Expression of Tropomyosin Causes Epithelial-Mesenchymal Transition of Lens Epithelial Cells (2011)
  • Author(s): Eri Kubo, Nilia Hasanova, Dhirendra P Singh, Yoshihiro Takamura, Yoshio Akagi
  • Organizer: ARVO 2011 Annual Meeting
  • Place of Presentation: Fort Lauderdale, FL, USA
• [Presentation] 白内障予防と治療の可能性 (2011)
  • Author(s): 久保江理
  • Organizer: 第31回金沢医科大学眼科研究会(招待講演)
  • Place of Presentation: 金沢市アートホール