Gene correction and transplantation into pathology model marmoset of iPS cells from retinitis pimentosa patient
Project/Area Number |
23592616
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Ophthalmology
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Research Institution | Saitama Medical University (2013) Keio University (2011-2012) |
Principal Investigator |
YOSHIDA Tetsu 埼玉医科大学, 医学部, 助教 (00365438)
|
Co-Investigator(Kenkyū-buntansha) |
OZAWA Yoko 慶應義塾大学, 医学部, 講師 (90265885)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 網膜色素変性症 / iPS細胞 / 再生医療 / 網膜 |
Research Abstract |
Retinitis pigmentosa (RP) is a kind of neurodegenerative diseases, which causes degeneration of rod cells in retina. In this work, we analyzed the mechanisms of the rod cells death and screened reagents which inhibited the degeneration. First of all, we developed a iPS cell line from an RP patient which a rhodopsin mutation and then induced rod cell differentiation. The rod cells with a rhodopsin mutation had more apoptotic cells than the cells without rhodopsin mutations. Next, we found that a reagent called 'rapamycin' inhibited the cell death of the rod cells, indicating that prescribing rapamycin to RP patient, which were caused by rhodopsin mutations, could inhibit the progress of the conditions of the disease.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] The use of induced pluripotent stem cells to reveal pathogenic gene mutations and explore treatments for retinitis pigmentosa2014
Author(s)
Yoshida T, Ozawa Y, Suzuki K, Yuki K, Ohyama M, Akamatsu W, Matsuzaki Y, Shimmura S, Mitani K, Tsubota K, Okano H
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Journal Title
Mol. Brain
Volume: (in press)
Related Report
Peer Reviewed
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[Presentation] An analysis of the mechanisms of degradation of photoreceptor cells in a retinitis pigmentosa patient using iPS cells2012
Author(s)
Tetsu Yoshida, Yoko Ozawa, Yuka Hirabayashi, Keiichiro Suzuki, Kohnosuke Mitani, Tetsuro Kobayashi, Manabu Ohyama, Masayuki Amagai, Yohei Okada, Wado Akamatsu, Kazuo Tsubota, Shigeto Shimmura, Hideyuki Okano
Organizer
International Society for Stem Cell Research (ISSCR) 10^<th> annual meeting
Place of Presentation
Pacifico Yokohama, Yokohama, Japan
Related Report
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[Presentation] An analysis of the mechanisms of degradation of photoreceptor cells in a retinitis pigmentosa patient using iPS cells.2012
Author(s)
Yoshida T, Ozawa Y, Suzuki K, Hirabayashi Y, Suzuki S, Koizumi H, Yuki K, Kobayashi T, Ohyama M, Amagai M, Okada Y, Akamatsu W, Matsuzaki Y, Mitani K, Shimmra S, Tsubota K, Okano H.
Organizer
International Society for Stem Cell Research (ISSCR)
Place of Presentation
Pacifico Yokohama, Yokohama, Japan.
Related Report
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[Presentation] A gene therapy for a gene mutation in human iPS cell using helper-dependent adenoviral vector2011
Author(s)
T. Yoshida, H. Koizumi, K. Yuki, Y. Hirabayashi, K. Suzuki, K. Mitani, K. Tsubota, S. Shimmura, Y. Ozawa, H. Okano
Organizer
The Association for Research in Vision and Ophthalmology (ARVO) 2011, The Future of Eye and Vision Research
Place of Presentation
Fort Lauderdale, Florida, USA
Related Report
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[Presentation] A gene therapy for a gene mutation in human iPS cell using helper-dependent adenoviral vector.
Author(s)
T. Yoshida, H. Koizumi, K. Yuki, Y. Hirabayashi, K. Suzuki, K. Mitani, ,K. Tsubota, S. Shimmura, Y. Ozawa, H. Okano.
Organizer
The Association for Research in Vision and Ophthalmology (ARVO)
Place of Presentation
Fort Lauderdale, Florida, USA.
Related Report